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Application of mesenchymal stem cell exosome preparation in treating alopecia areata

A technology of stem cells and exosomes, applied in the field of biomedicine, can solve the problems of low content of secreted factors, unstable properties, and difficult to store, and achieve the effects of improving skin tissue structure, convenient collection and simple ingredients.

Pending Publication Date: 2020-04-03
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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Problems solved by technology

However, factors such as low content of secreted factors, unstable properties, and difficulty in storage have limited its application.

Method used

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  • Application of mesenchymal stem cell exosome preparation in treating alopecia areata
  • Application of mesenchymal stem cell exosome preparation in treating alopecia areata
  • Application of mesenchymal stem cell exosome preparation in treating alopecia areata

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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0029] Isolation, culture and identification of umbilical cord mesenchymal stem cells (UC-MSCs)

[0030] Under sterile conditions, the umbilical cords of healthy fetuses with full-term pregnancy were taken, rinsed with saline to remove the outer mold of the umbilical cord tissue and the umbilical arteries, and then the remaining tissue was cut into pieces with a diameter of about 2-3 mm. Spread the small pieces evenly on the bottom of the culture bottle, dry at 37°C for 1 hour, and then slowly add an appropriate amount of DMEM / HAM’SF-12 complete medium.

[0031] When culturing for 5-7 days, the half-quantity liquid replacement method is used to change the medium. On the one hand, the toxic effect cau...

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Abstract

The invention relates to an application of a mesenchymal stem cell exosome preparation in treating alopecia areata. An exosome preparation includes exosome secreted by human umbilical cord mesenchymalstem cells and PBS. A preparation method of the exosome secreted by the human umbilical cord mesenchymal stem cells includes placing the umbilical cord mesenchymal stem cells separated from the umbilical cord of a full-term normal delivered healthy fetus to perform culture at 37 DEG C to P3; performing replacing in a DMEM / HAM'SF-12 culture medium without fetal bovine serum when the cells are fused to 60-80%, and performing starvation culture for 48 h, and then collecting the culture medium; performing centrifugation on the collected culture medium to remove the cells and cell debris, and extracting the exosome by using a ultracentrifugation method; and obtaining the mesenchymal stem cell exosome preparation UC-MSCs-Exo by performing conservation at -80 DEG C with a PBS re-suspended sediment. The UC-MSCs is low in preparation cost, abundant in source and convenient in collection and has no traumatic operation on human bodies; the UC-MSCs-Exo is simple in component, stable in cell factor and not easy to denature; and therefore, the skin tissue structures at alopecia areata parts can be improved, and the damaged hair follicles can be repaired or reconstructed.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the technology of preparing exosomes from mesenchymal stem cells, in particular to the application of a mesenchymal stem cell exosome preparation in the treatment of alopecia areata. Background technique [0002] Hair loss has become a common "modern disease" nowadays. Although it is not a serious disease, it brings great trouble and inconvenience to people's life. The causes of hair loss and the types of hair loss are different, and the reasons for a considerable part of hair loss are not yet clear. Alopecia areata is an autoimmune non-scarring pattern of hair loss. Also known as circular hair loss, round baldness, ghost shaving head. If all the hair falls out, it is called alopecia totalis, and if all the hair on the whole body falls out, it is called alopecia universalis. There is no other abnormality in the skin of the ward except hairlessness. In the pathological examination of a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P17/14C12N5/0735
CPCA61K35/28A61P17/14C12N5/0665C12N2509/10
Inventor 程国钢王倩邢紫钧詹举明赵刚马洁
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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