A strain of Torula daerkeii producing α-farnesene and its fermentation method
A technology of Torula sporogenes and farnesene, which is applied in the field of Derkesporia sporogenes and its fermentation, can solve problems such as low content, and achieve the effects of high capacity, increasing content, and increasing flavor and nutritional value.
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Embodiment 1
[0031] Embodiment 1: the screening and identification of the Torulaspora delbrueckii of high yield α-farnesene
[0032] Take 10g of highland barley Daqu and dissolve it in 90mL of sterile saline, shake it on a shaker at 30°C for 30 minutes, then carry out gradient dilution, and the selected dilution is 10 -2 、10 -3 、10 -4 、10 -5 After coating the WL solid plate and culturing at 30°C for 48 hours, the colony morphology of the Torulaspora delbrueckii plate is as follows: figure 1 shown.
[0033] Pick a single colony that meets the phenotypic characteristics and put it into a 25mL test tube filled with 5mL of highland barley juice medium, at natural pH, 30°C, 200rpm, and cultivate for 36h to become a seed culture solution. The cultivated seed culture solution was inoculated into a 250mL Erlenmeyer flask containing 50mL of highland barley juice medium with 5% inoculation amount, and fermented for 48h at natural pH, 30°C, and 200rpm. Three strains with higher α-farnesene produ...
Embodiment 2
[0037] Example 2: Comparison of α-farnesene produced by Torulaspora delbrueckii A7 and other yeasts
[0038] The bacterial strain that present embodiment comprises has Saccharomyces cerevisiae (Sc), Hyphopichiaburtonii (Hb), Kazachstania unispora (Ku), Wickerhamomyces anomalus (Wa), Hanseniaspora uvarum (Hu), Pichia kudriavzevi (Pku), Pichia scaptomyzae (Psc), Pichia membranifaciens (Pme) and Pichia manshurica (Pma), and the strain of the invention Torulaspora delbrueckii A7 (Td A7). These 10 strains all come from the brewing environment of highland barley wine.
[0039] Pick a single colony of the above 10 strains into a 25mL test tube filled with 5mL barley juice medium, cultivate at natural pH, 30°C, 200rpm for 36h to become a seed culture solution. Inoculate the cultivated seed culture solution with 5% inoculum in a 250mL Erlenmeyer flask containing 50mL barley juice, and ferment for 48h at natural pH at 30°C. HS-SPME and GC-MS techniques were used to detect the α-Farnes ...
Embodiment 3
[0043] Example 3: Detection of physiological and biochemical properties of Torulaspora delbrueckii A7
[0044] Temperature tolerance test: Pick the Torulaspora delbrueckii A7 strain obtained in Example 1, inoculate it in 5ml YPD liquid medium, and culture it at 30°C for 36h to OD 800 In 1.2-1.4. Dilute medium OD with sterile saline 800 To 1, inoculate 0.5mL bacterial liquid into 50ml YPD liquid medium. They were cultured at different temperature gradients of 20°C, 25°C, 30°C, 37°C, 40°C, 42°C and 46°C for 36 hours. The results show that the Torulaspora delbrueckii A7 bacterial strain of the present invention can grow in the temperature range of 20-46°C, the optimum temperature is 25-35°C, and the OD of 36h is cultivated at 25-35°C 800 Greater than 1.
[0045] Acidity tolerance test: Pick the Torulaspora delbrueckii A7 strain obtained in Example 1, inoculate it in 5ml YPD liquid medium, and culture it at 30°C for 36h to OD 800 In 1.2-1.4. Dilute medium OD with sterile sal...
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