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A kind of treatment method of foam in microbial fermentation process

A technology of microbial fermentation and treatment method, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of limiting the application effect of defoamer, reducing the yield of target products, increasing production costs, etc. The foaming effect is remarkable, the yield and quality are improved, and the effect of improving the effect

Active Publication Date: 2020-11-20
鹤山市东古调味食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the reasons of composition or structure, as well as physical and chemical properties, some existing chemically synthesized defoamers only defoam without suppressing foam, some have poor effect, and some will have an impact on fermenting microbial strains, resulting in increased production costs. Reduce the yield of the target product, etc., thus limiting the application effect of the defoamer

Method used

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  • A kind of treatment method of foam in microbial fermentation process
  • A kind of treatment method of foam in microbial fermentation process

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for treating foam in a microbial fermentation process, comprising the following steps:

[0029] (1) Sterilize the defoamer at 110°C for 45 minutes;

[0030] (2) When the foam produced by fermentation reaches 1% of the total volume, add a defoamer with a volume of 0.1%;

[0031] (3) When stirring until the foam height is stable, add 0.01% defoamer again;

[0032] The weight ratio of each component of the defoamer is: 6 parts of polyether modified silicone oil, 3 parts of polyoxypropylene tetrahydric alcohol ether, 3 parts of sodium lauryl sulfate, 2 parts of sodium alginate, Potassium acid 1 part and deionized water 85 parts.

[0033] The preparation method of the polyoxypropylene tetrahydric alcohol ether comprises: taking 150 parts of pentaerythritol and 2 parts of sodium hydroxide and adding them to a polymerization reactor for mixing, dehydration, feeding 3000 parts of propylene oxide under the protection of nitrogen, and controlling the reaction temperatu...

Embodiment 2

[0039] A method for treating foam in a microbial fermentation process, comprising the following steps:

[0040] (1) Sterilize the defoamer at 120°C for 40 minutes;

[0041] (2) When the foam produced by fermentation reaches 3% of the total volume, add a defoamer with a volume of 0.2%;

[0042] (3) When stirring until the foam height is stable, add 0.05% defoamer again;

[0043] The weight ratio of each component of the defoamer is: 10 parts of polyether modified silicone oil, 5 parts of polyoxypropylene tetrahydric alcohol ether, 4 parts of sodium lauryl sulfate, 3 parts of sodium alginate, Potassium acid 1.6 parts and deionized water 76.4 parts; That is, the weight ratio of polyether modified silicone oil and sodium alginate is 10:3, that is, 3.33:1.

[0044] The preparation method of the polyoxypropylene tetrahydric alcohol ether comprises: taking 150 parts of pentaerythritol and 2 parts of sodium hydroxide and adding them to a polymerization reactor for mixing, dehydratio...

Embodiment 3

[0050] A method for treating foam in a microbial fermentation process, comprising the following steps:

[0051] (1) Sterilize the defoamer at 118°C for 30 minutes;

[0052] (2) When the foam produced by fermentation reaches 5% of the total volume, add a defoamer with a volume of 0.3%;

[0053] (3) When stirring until the foam height is stable, add 0.08% defoamer again;

[0054]The weight ratio of each component of the defoamer is: 12 parts of polyether modified silicone oil, 8 parts of polyoxypropylene tetrahydric alcohol ether, 5 parts of sodium lauryl sulfate, 5 parts of sodium alginate, Potassium acid 2 parts and deionized water 68 parts.

[0055] The preparation method of the polyoxypropylene tetrahydric alcohol ether comprises: taking 150 parts of pentaerythritol and 2 parts of sodium hydroxide and adding them to a polymerization reactor for mixing, dehydration, feeding 3000 parts of propylene oxide under the protection of nitrogen, and controlling the reaction temperat...

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Abstract

The invention provides the technical field of microorganisms, and particularly relates to a foam treatment method in a microbial fermentation process. The treatment method comprises the step that a defoaming agent is added into a fermentation system in twice, wherein the defoaming agent comprises polyether modified silicone oil, polyoxypropylene tetrahydric alcohol ether, lauryl sodium sulfate, sodium alginate, potassium sorbate and deionized water. By adjusting the ratio of polyether modified silicone oil to sodium alginate in the defoaming agent, the defoaming and foam inhibition effects areobviously improved. The method provided by the invention can quickly eliminate foam, has a remarkable foam inhibition effect, and has no adverse side effect on microbial strains. The yield and quality of a product are effectively improved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for treating foam in a microorganism fermentation process. Background technique [0002] Microbial fermentation is a complex biochemical process. In this production process, many factors need to be regulated, such as culture medium, sterilization time, temperature, pH value, oxygen supply, etc. Foam control is also an important one. item. Foam is a dispersion system in which a large amount of gas is dispersed in a liquid, the dispersed phase is gas, and the continuous phase is fermentation broth. The generation of a large amount of foam will cause great harm to the process production, such as: reducing output, affecting product quality, and affecting normal production. Therefore, the treatment of foam during fermentation is particularly important. [0003] In the process of microbial fermentation, there are two commonly used defoaming methods: one is to add def...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/54C12P13/06B01D19/04C12R1/02C12R1/15
CPCB01D19/0404B01D19/0409B01D19/0422B01D19/0495C12P7/54C12P13/06
Inventor 任达洪
Owner 鹤山市东古调味食品有限公司
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