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Efficient and aseptic sowing method of Hydrangea macrophylla (Thunb.) Ser.

A technology for aseptic seeding and hydrangea, which is applied in the field of plant cultivation and reproduction, can solve the problems of difficult operation, high pollution rate, and low germination rate, and achieve the effects of small seed damage, low pollution rate, and high germination rate

Pending Publication Date: 2020-04-10
HANGZHOU LANDSCAPING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hydrangea aseptic sowing studies reported at home and abroad all use fruit slices or artificially peeled off the seeds. Because the hydrangea seeds are very small, the existing aseptic sowing methods are difficult to operate, have high pollution rates, and low germination rates, and cannot be used for the development of new hydrangea varieties. Cultivation provides a large amount of material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] Embodiment object: Hydrangea big leaf artificially pollinated fruit

[0026] Step 1) The female parent used for artificial pollination is a container seedling, which is carried out from April to May. After pollination, the female parent is placed in the greenhouse, and 3 g of Aolu A2 slow-release fertilizer is applied. The whole plant was sprayed with 500 carbendazim solution every 7 days. About 3 months later, when the sepals are dehydrated and dry, and the fruit is swollen and not dry, collect the plump fruit. The collected fruit is wiped clean with an alcohol cotton ball, put into a mixed solution of detergent and 10% sodium hypochlorite, stirred on a magnetic stirrer for 30 minutes, and then rinsed with tap water for 1 hour for later use;

[0027] Step 2) Soak in 75% alcohol for 10-15 seconds on the ultra-clean workbench. After pouring off the alcohol, soak in 0.1% mercuric acid solution for 25 minutes. During this time, shake continuously. After pouring out the me...

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PUM

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Abstract

The invention provides an efficient and aseptic sowing method of Hydrangea macrophylla (Thunb.) Ser.. The method includes the following steps: 1) about 3 months after artificial pollination or naturalpollination, picking plump fruits, and washing the picked fruits for later use; 2) soaking the fruits into an alcohol solution and a mercuric chloride solution for disinfection on a clean bench, andperforming rinsing; 3) laying an allochroic silica-gel desiccant in a petri dish on the clean bench, and covering the allochroic silica-gel desiccant with filter paper; 4) airing the disinfected fruits, laying the fruits evenly on the filter paper in the step 3), covering the petri dish with a petri dish cover, putting the petri dish into a dryer, and performing drying; 5) after sufficient drying,taking the fruits out on the clean bench, cutting tops of the fruits by using a knife, squeezing the fruits softly by using a tweezers so as to squeeze seeds out, and spreading the seeds evenly in agermination culture medium for culture; 6) after culture for a period of time, transferring the germinated seedlings to a rooting culture medium for culture; 7) after a period of cultivation and formation of developed root systems of the seedlings, placing the seedlings in a greenhouse for domestication; and 8) after domestication of a period of time, washing the seedlings, and exercising the seedlings. The method has simple operation, a low pollution rate, a high rate of seedling emergence and a short seedling cycle, and is suitable for Hydrangea macrophylla, Hydrangea paniculata Sieb., Hydrangea strigosa Rehd. and most species and varieties of Hydrangea.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation and reproduction, and in particular relates to a hydrangea high-efficiency aseptic sowing method. Background technique [0002] Hydrangea belongs to the genus Hydrangea of ​​Saxifragaceae (Saxifragaceae). It has a long flowering period, a wide variety of varieties, strong adaptability, cold resistance, shade tolerance, strong resistance, less pests and diseases, and easy maintenance. It is highly respected by the industry and widely cultivated in the world. [0003] Most of the existing hydrangea varieties are foreign varieties, and there are few varieties cultivated in China. The low sowing efficiency is one of the important reasons. Traditional sowing is affected by various reasons such as seed maturity, substrate, water content, diseases and insect pests, etc., resulting in low seedling rate and long cycle. Aseptic seeding is an effective way to solve these problems. The hydrangea ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/28A01G24/15
CPCA01H4/001A01H4/008A01G24/15A01G24/28
Inventor 邱帅高凯彭悠悠魏建芬沈柏春孙丽娜杨浩洪毛柳
Owner HANGZHOU LANDSCAPING
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