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Preparation and application of antibacterial modified exosome burn wound healing-promoting biological dressing

A biological dressing and exosome technology, which is applied in drug delivery, medical science, absorbent pads, etc., can solve the problems of exosomes lack of antibacterial function and limited effect, and achieve the promotion of wound healing, good wound healing, and good air permeability Effect

Inactive Publication Date: 2020-04-10
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of antibacterial function of exosomes, their role in the treatment of infected wounds is very limited.

Method used

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  • Preparation and application of antibacterial modified exosome burn wound healing-promoting biological dressing
  • Preparation and application of antibacterial modified exosome burn wound healing-promoting biological dressing
  • Preparation and application of antibacterial modified exosome burn wound healing-promoting biological dressing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 The preparation of antibacterial modified exosome burn wound healing biological dressing sample 1 (the synthesis process is as follows Figure 5 shown)

[0054] (1) Collect the stem cell culture medium, and centrifuge the collected cell culture supernatant at 4° C. and 2000 g to remove cell debris and dead cells in the supernatant. The centrifuged supernatant was collected and filtered through a 0.22 μm filter membrane. Place the filtered culture supernatant in an ultrafiltration centrifuge tube (30KD), centrifuge at 4°C and 5000g for 20min, and collect the ultrafiltered liquid;

[0055] (2) Pass the ultrafiltration liquid collected in step (1) through the qEV-exosome separation column, and place a 15ml centrifuge tube under the column for collecting the isolated exosomes. Before extracting exosomes, rinse with 10ml of PBS, add 0.5ml of ultrafiltered cell culture supernatant, discard 3ml of waste liquid, and start collecting 2ml of exosome solution. At this ...

Embodiment 2

[0061] Example 2 Preparation of antibacterial modified exosome burn wound healing biological dressing sample 2

[0062] (1) Collect the stem cell culture medium, and centrifuge the collected cell culture supernatant at 4° C. and 2000 g to remove cell debris and dead cells in the supernatant. The centrifuged supernatant was collected and filtered through a 0.22 μm filter membrane. Place the filtered culture supernatant in an ultrafiltration centrifuge tube (30KD), centrifuge at 4°C and 5000g for 20min, and collect the ultrafiltered liquid;

[0063] (2) Pass the ultrafiltration liquid collected in step (1) through the qEV-exosome separation column, and place a 15ml centrifuge tube under the column for collecting the isolated exosomes. Before extracting exosomes, wash them with 10ml of PBS, add 0.5ml of ultrafiltered cell culture supernatant, discard 3ml of waste liquid, and start collecting 2ml of exosomes. At this time, pay attention to supplementing PBS above the column to pr...

Embodiment 3

[0068] Example 3 Preparation of antibacterial modified exosome burn wound healing biological dressing sample 3

[0069] (1) Collect the stem cell culture medium, and centrifuge the collected cell culture supernatant at 4° C. and 2000 g to remove cell debris and dead cells in the supernatant. The centrifuged supernatant was collected and filtered through a 0.22 μm filter membrane. Place the filtered culture supernatant in an ultrafiltration centrifuge tube (30KD), centrifuge at 4°C and 5000g for 20min, and collect the ultrafiltered liquid;

[0070] (2) Pass the ultrafiltration liquid collected in step (1) through the qEV-exosome separation column, and place a 15ml centrifuge tube under the column for collecting the isolated exosomes. Before extracting exosomes, rinse with 10ml of PBS, add 0.5ml of ultrafiltered cell culture supernatant, discard 3ml of waste liquid, and start collecting 2ml of exosome solution. At this time, pay attention to supplementing PBS above the column. ...

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Abstract

Preparation and application of an antibacterial modified exosome burn wound healing-promoting biological dressing are disclosed. A raw dressing is a novel artificially modified exosome prepared by taking cell-derived exosome as a carrier of an antibacterial agent and combining with a broad-spectrum antibacterial agent; and the modified exosome is loaded into an asymmetric chitosan porous moisturizing dressing. The weight ratio of chitosan to water-absorbing molecules to the exosome to the antibacterial agent in the dressing is (40-60): (20-40): (5-20): (1-10). The dressing has a broad-spectrumantibacterial effect, a blood vessel repair promoting effect, a nerve repair promoting effect and a wound healing promoting effect. Meanwhile, the dressing has moisture retention, air permeability, barrier property, high strength and easy disclosure, and is a novel antibacterial compound dressing with a broad-spectrum antibacterial effect. The dressing is prepared through freeze-drying, has advantages of simple preparation process, low cost, outstanding treatment effect and good safety, and has remarkable medical value and industrialization potential.

Description

technical field [0001] The invention relates to an antibacterial modified exosome burn wound healing-promoting biological dressing, in particular to a water-absorbing antibacterial and healing-promoting hydrogel antibacterial dressing for infectious wounds. Background technique [0002] The skin is the largest organ of the human body. It not only protects the body from damage, isolates pathogens, and acts as a barrier protection, but also plays an important role in preventing excessive water loss from the body. Trauma often damages normal skin tissue structure and physiological functions. The infection caused by the wound during the treatment process has always been the main problem in the clinical treatment process. Pseudomonas aeruginosa (P. aeruginosa, a typical Gram-negative bacterium) is a widely distributed microorganism in nature, and it is also one of the main pathogens of nosocomial infection. Wound infection caused by Pseudomonas aeruginosa invasion into the woun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/18A61L15/28A61L15/32A61L15/26A61L15/24A61L15/20A61L15/42A61L15/44A61L15/46
CPCA61L15/18A61L15/20A61L15/24A61L15/26A61L15/28A61L15/32A61L15/325A61L15/40A61L15/42A61L15/44A61L15/46A61L2300/102A61L2300/104A61L2300/206A61L2300/236A61L2300/404A61L2300/412A61L2300/602A61L2400/04C08L5/08C08L89/00C08L71/02C08L33/26C08L33/12C08L29/04
Inventor 刘海峰钱智勇樊瑜波白雅婷
Owner BEIHANG UNIV
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