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A kind of method for producing ethanol and carboxylic acid simultaneously and its application

A carboxylic acid and ethanol technology, applied in the fields of fermentation and biology, can solve problems such as hindering the industrialization process and increasing production costs, and achieve the effect of low cost and cost reduction

Active Publication Date: 2022-07-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because this method requires additional addition of ethanol as an electron donor, the production cost of producing hexanoic acid by this method is greatly increased, and the industrialization process of producing hexanoic acid by this method is hindered.

Method used

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  • A kind of method for producing ethanol and carboxylic acid simultaneously and its application
  • A kind of method for producing ethanol and carboxylic acid simultaneously and its application
  • A kind of method for producing ethanol and carboxylic acid simultaneously and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Production of ethanol-type acidified fermentation broth

[0063] Specific steps are as follows:

[0064] The experiment was divided into three groups, namely, inoculated yeast group, natural acidification group and inoculated sludge group, among which:

[0065] Yeast inoculation group: first add water to the fruit and vegetable waste to obtain a fermentation system with a total solid concentration (TS) of 8% and a volatile solid (VS) content of 78 g-VS / L, and then at 1:5000 (m / m ) in the fermentation system after inoculating yeast (Angel dry yeast powder), the fermentation system is fermented for 17d under the conditions that the temperature is 34-36°C, the pH is 4.0-4.5, and the rotational speed is 60rpm, to obtain a fermentation liquid, and the fermentation liquid.

[0066] Natural acidification group: first add water to the fruit and vegetable waste to obtain a fermentation system with a total solids concentration (TS) of 8% and a volatile solids (VS) co...

Embodiment 2

[0084] Example 2: Application of ethanol-type acidified fermentation broth

[0085] Specific steps are as follows:

[0086] The fermentation broth produced by the natural acidification group of Example 1 was inoculated with anaerobic sludge with an inoculum of 30 g-VS / L to obtain a fermentation system; the fermentation system was subjected to a temperature of 30°C, a pH of 4.5, and a rotating speed of 60rpm. Fermented for 720h to obtain fermentation broth.

[0087] The content of caproic acid in the fermentation broth was detected, and the detection result was: the fermentation broth contained 13.7 g / L caproic acid.

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Abstract

The invention discloses a method for simultaneously producing ethanol and carboxylic acid and its application, belonging to the field of fermentation technology and biotechnology. The present invention provides a method for simultaneously producing ethanol and carboxylic acid. In the method, the ethanol containing both ethanol and carboxylic acid can be obtained simply by self-fermenting unsterilized fruit and vegetable waste under certain conditions. Acidified fermentation broth. Using this method to ferment for 300 hours, the content of ethanol, acetic acid, butyric acid and lactic acid in the ethanolic acidified fermentation broth can be as high as 27.5, 8.23, 0.54 and 0.5 g / L, respectively. In the ethanol-type acidified fermentation broth obtained by this method, the COD equivalent ratio of ethanol and carboxylic acid is 4-7:10-12. Therefore, the ethanol-type acidified fermentation broth obtained by this method can be directly inoculated with anaerobic sewage. After the mud is used for the fermentation production of caproic acid, there is no need to add ethanol as an electron donor, which greatly reduces the cost of caproic acid fermentation.

Description

technical field [0001] The invention relates to a method for simultaneously producing ethanol and carboxylic acid and its application, belonging to the field of fermentation technology and biotechnology. Background technique [0002] Caproic acid is a medium chain fatty acid containing six carbon atoms (C 5 H 11 COOH), with strong hydrophobicity and high energy density, is an important chemical raw material that can be directly used in the production of animal feed, green antibacterial agents and corrosion inhibitors. Its ester products are widely used in food flavor, resin, rubber, pharmaceutical and other industries. In addition, caproic acid is a synthetic precursor of biofuels, which can be indirectly converted into biodiesel or jet fuel by means of organic chemistry. [0003] The production methods of hexanoic acid can be divided into three types: chemical synthesis, natural product extraction and microbial catalysis. Among them, the chemical synthesis method mainly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P7/40C12P7/52C12P7/54C12P7/56C12R1/01C12R1/645
CPCC12P7/10C12P7/40C12P7/54C12P7/52C12P7/56Y02E50/10
Inventor 黄振兴余江南廖家林阮文权
Owner JIANGNAN UNIV