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Brain tissue stationary liquid, preparation method and application method thereof

A fixation method, brain tissue technology, applied in application, preparation of test samples, preservation of human or animal bodies, etc., can solve problems such as poor dehydration effect, poor treatment effect, high brittleness, etc., and achieve good fixation effect, staining Vibrant, good dyeing effect

Active Publication Date: 2020-04-10
江苏博赛孚医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Excessive water content in brain tissue will cause it to be more brittle, easily broken, and difficult to make tablets
The dehydration effect of traditional brain tissue fixative is poor
It is less effective for some brain tissues with higher water content, such as infant brain tissue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Prepare brain tissue fixative:

[0035] Mix 55 parts of formaldehyde, 3 parts of sodium chloride, 40 parts of glycerin and 200 parts of distilled water by ultrasonic to obtain liquid a, wherein the ultrasonic power is 200W, the ultrasonic time is 15min, and the mixing temperature is room temperature;

[0036] Fully mix 26 parts of methanol, 140 parts of ethanol and 46 parts of tert-butanol to obtain liquid b;

[0037] The liquid a and the liquid b were mixed uniformly by ultrasound to obtain a brain tissue fixative, wherein the ultrasonic power was 300W, the ultrasonic time was 25min, and the mixing temperature was 30°C.

[0038] Fixation of Brain Tissue:

[0039] Place the brain tissue sample in a cryostat, control the temperature of the box to -18°C, and the temperature of the sample head of the refrigeration table to -16°C, completely wrap the tissue with OCT cryogel, and when the brain tissue and its surface OCT cryogel harden, perform Slicing to obtain a frozen s...

Embodiment 2

[0043] Prepare brain tissue fixative:

[0044] 45 parts of formaldehyde, 5 parts of sodium chloride, 30 parts of glycerin and 300 parts of distilled water were mixed uniformly by ultrasonic to obtain a liquid, wherein the ultrasonic power was 200W, the ultrasonic time was 10min, and the mixing temperature was room temperature;

[0045] Fully mix 34 parts of methanol, 120 parts of ethanol and 58 parts of tert-butanol to obtain liquid b;

[0046] The liquid a and the liquid b were mixed uniformly by ultrasound to obtain a brain tissue fixative, wherein the ultrasonic power was 300W, the ultrasonic time was 20min, and the mixing temperature was 30°C.

[0047] Fixation of Brain Tissue:

[0048] Place the brain tissue sample in a cryostat, control the temperature of the box to -18°C, and the temperature of the sample head of the refrigeration table to -16°C, completely wrap the tissue with OCT cryogel, and when the brain tissue and its surface OCT cryogel harden, perform Slicing ...

Embodiment 3

[0052] Prepare brain tissue fixative:

[0053] 52 parts of formaldehyde, 3.5 parts of sodium chloride, 38 parts of glycerin and 240 parts of distilled water were uniformly mixed by ultrasonic to obtain liquid a, wherein the ultrasonic power was 200W, the ultrasonic time was 14min, and the mixing temperature was room temperature;

[0054] Fully mix 28 parts of methanol, 135 parts of ethanol and 50 parts of tert-butanol to obtain liquid b;

[0055] The liquid a and the liquid b were mixed uniformly by ultrasound to obtain a brain tissue fixative, wherein the ultrasonic power was 300W, the ultrasonic time was 24min, and the mixing temperature was 30°C.

[0056] Fixation of Brain Tissue:

[0057] Place the brain tissue sample in a cryostat, control the temperature of the box to -18°C, and the temperature of the sample head of the refrigeration table to -16°C, completely wrap the tissue with OCT cryogel, and when the brain tissue and its surface OCT cryogel harden, perform Slicin...

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Abstract

The invention discloses a brain tissue stationary liquid, a preparation method and an application method thereof. The preparation method of the brain tissue stationary liquid comprises the following steps of: ultrasonically and uniformly mixing 45-55 parts of formaldehyde, 3-5 parts of sodium chloride, 30-40 parts of glycerol and 200-300 parts of distilled water to obtain a solution a; fully and uniformly mixing 26-34 parts of methanol, 120-140 parts of ethanol and 46-58 parts of tert-butyl alcohol to obtain a solution b; and ultrasonically mixing the solution a and the solution b uniformly toobtain the brain tissue stationary liquid. The brain tissue fixation method comprises the following steps of: freezing and slicing a brain tissue sample to obtain frozen brain tissue slices; standingthe frozen brain tissue slices at room temperature for 20-40 seconds, then adding the brain tissue stationary liquid into the frozen brain tissue slices, and conducting fixing treatment for 2 minutes; and dyeing the fixed frozen brain tissue slices within 10 minutes. By the adoption of the technical scheme, the basic form of cells can be kept normal, the cells do not shrink obviously, meanwhile,brain tissue has good dyeing performance, the tissue structure is clear after dyeing, and cell nucleus dyeing is bright.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a brain tissue fixative and its preparation method and application method. Background technique [0002] With the improvement of medical technology, many brain tumors have been clearly diagnosed and treated surgically, and brain tissue frozen section technology and correct pathological diagnosis are powerful guarantees for surgical operations. Cryosection is a method of preparing slices by freezing the brain tissue and quickly sectioning and staining it. The fixation of the brain tissue is the key to the preparation of the frozen section of the brain tissue. [0003] Brain tissue normally contains a certain amount of water. Excessive water content in the brain tissue will cause it to be more brittle, easily broken, and difficult to make tablets. Traditional brain tissue fixatives have poor dehydration effects. For some brain tissues with high water content, such as infant brain t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30A01N1/00
CPCG01N1/30A01N1/00
Inventor 王守立赵飞沈刚
Owner 江苏博赛孚医疗科技有限公司
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