Kudzu vine root polysaccharide as well as preparation method and application thereof

A technology of pueraria polysaccharides and polysaccharides is applied in the preparation of pueraria homogeneous polysaccharides, the preparation of lipid-lowering drugs, and the fields of polysaccharides, which can solve the problems of unclear medicinal substances and the like.

Active Publication Date: 2020-04-14
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gegen is mainly used clinically for patients with diabetes and hyperlipidemia, such as Gegen Decoction, Gegen Qinlian Decoction and other cla

Method used

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  • Kudzu vine root polysaccharide as well as preparation method and application thereof
  • Kudzu vine root polysaccharide as well as preparation method and application thereof
  • Kudzu vine root polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of kudzu root polysaccharide QL

[0020] Pueraria 7.0Kg is pulverized, extracted 3 times with 90°C water at a solid-to-liquid ratio of 1:20, filtered, combined with extracts, and concentrated to a density of 1.1-1.2; then add 2 times the volume of absolute ethanol and let it stand for about 3 hours , filtered to obtain a precipitate, redissolve the precipitate in water, add 0.1% activated carbon to remove the pigment, centrifuge, concentrate the supernatant to 0.5-1L, freeze-dry to obtain fluffy kudzu root polysaccharide 100-244g; weigh Add 200g of crude polysaccharide with distilled water and magnetically stir to dissolve, centrifuge, add the supernatant to a macroporous resin column HP-20 for separation, elute with pure water, 10% ethanol, and 20% ethanol solution, collect each fraction, and analyze according to sulfuric acid- The same fractions were combined by phenol color development and 490 nm detection results, then concentrated, dialyzed a...

Embodiment 2

[0023] Example 2: Structural characterization of kudzu root polysaccharide (QL)

[0024] (1) Determination of molecular weight

[0025] Using a TSK-GEL GMPWXL gel column (300×7.6 mm), the mobile phase is 0.01 mol / L NaNO3, the flow rate is 0.8mL / min, the column temperature is 25°C, and a series of molecular weight dextran is used as standard song. The measured kudzu root Polysaccharide QL has a molecular weight of 10-60 KDa.

[0026] (2) Determination of total sugar, uronic acid and protein content

[0027] The sulfuric acid-phenol method determined the total sugar content of QL to be 98.7%;

[0028] The uronic acid content of QL measured by m-hydroxybiphenyl method is 0.0%;

[0029] The protein content of QL determined by Coomassie Brilliant Blue method was 0.64%;

[0030] (3) Sugar composition analysis

[0031] QL was hydrolyzed by 2.0 mol / L TFA at a constant temperature of 100°C for 6 hours, and the product obtained by complete hydrolysis was reduced by adding an approp...

Embodiment 3

[0036] Embodiment 3: In vivo lipid-lowering experimental method

[0037] Fifty-six male Wistar rats were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (8 weeks, body weight 200±20 g). All animals were fed with normal diet for 1 week. Subsequently, 8 rats were used as the blank control group (abbreviated as the blank group (1) for short), fed with normal diet, and the remaining 48 rats were fed with high-fat diet (HF, 88.5% of common rat diet with 1.2% cholesterol, 0.3% cholic acid sodium, 10% lard) for 2 months to establish a hyperlipidemia model.

[0038]Rat plasma levels of TG, TC, LDL-C and HDL-C were sampled every half month. After 2 months, all rats were anesthetized with isoflurane and blood samples were collected from orbital veins using capillary tubes. Centrifuge the blood sample (4°C; 4,000 r×min-1; 15min) to obtain plasma samples, use the kit (purchased from Nanjing Jian Biotechnology Co., Ltd., China) to make a standard curve, and ...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and relates to preparation and lipid-lowering activity of homogeneous polysaccharides in a kudzu vine water extract. In-vivo experiments prove that the kudzu vine homogeneous polysaccharide QL separated from a Jiangxi genuine medicinal material namely kudzu vine has remarkable lipid-lowering activity, remarkably reduces the content of serum triglyceride and liver indexes, and can be used for developing potential safe and effective lipid-lowering medicines.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine and relates to polysaccharides, in particular to a preparation method of kudzu root homogeneous polysaccharides and its use in the preparation of lipid-lowering drugs. Background technique [0002] With the improvement of people's living standards, excessive intake of high-fat food makes hyperlipidemia tend to develop younger, and it is also one of the main risk factors for coronary heart disease. In the regulation of cholesterol metabolism In related research, traditional Chinese medicine is playing an increasingly important role. Clinically, for hyperlipidemia, Western medicine statins (such as simvastatin, etc.) are mainly used for treatment, which requires long-term medication and has significant side effects, such as rhabdomyolysis. Therefore, it is efficient and pertinent to search for safe and effective lipid-lowering drugs from traditional Chinese medicine. [0003] Pueraria l...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61K36/488A61P3/06
CPCC08B37/0003C08B37/0006A61K31/715A61K36/488A61P3/06A61K2236/00C08B37/0009A61K31/716
Inventor 温泉朱卫丰欧阳辉冯育林刘荣华钱凯杜惠
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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