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Hepatitis C virus nucleic acid detection sample treatment method

A technology of hepatitis C virus and processing method, which is applied in the field of processing of hepatitis C virus nucleic acid detection samples, can solve problems such as increased patient suffering, waste of personnel and reagent costs, and experimental failure, so as to avoid false negative results and improve accuracy Sexuality, the effect of ensuring the success rate of the experiment

Active Publication Date: 2020-04-14
JILIN JINYU MEDICAL SCI INSPECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, blood samples are usually not in a very ideal health condition. When the state of serum samples is jaundice, hemolysis, lipemia, etc., this special sample will be affected by red blood cells, bilirubin, fat particles, etc. during detection, inhibiting HCVRNA Extraction, resulting in false negative results, at least the experiment fails, and the patient needs to be re-sampled, resulting in a waste of personnel and reagent costs, increasing the pain of sampling for the patient, and at the worst, causing misjudgment of the result and delaying the best treatment time for the patient

Method used

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  • Hepatitis C virus nucleic acid detection sample treatment method
  • Hepatitis C virus nucleic acid detection sample treatment method
  • Hepatitis C virus nucleic acid detection sample treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The sample processing method of the existing hepatitis C virus nucleic acid detection kit (Hunan Shengxiang Biotechnology Co., Ltd.), the steps are as follows:

[0039] 1. Take 10 cases of serum samples distributed in four categories, numbered 1-10, wherein, samples 1-9 are abnormal samples by visual inspection, and 10 is a sample (control), such as Figure 5 shown;

[0040] 2. Take an appropriate amount of 1.5ml sterilized centrifuge tubes, label the negative control, positive control, quantitative reference A-D and the sample to be tested respectively, and add 600 μl of extraction reagent 1 mixture to each tube;

[0041] 3. Add 200 μl of the sample to be tested, negative control, positive control, and quantitative reference products A-D to each tube, cover the tube cap, shake and mix for 10 seconds, and centrifuge briefly;

[0042] 4. Add 100 μl extraction reagent 2 to each tube, vortex and mix for 10 seconds, then let stand at room temperature for 30 minutes;

[00...

Embodiment 2

[0047] The processing method of hepatitis C virus nucleic acid detection sample of the present invention, the steps are as follows:

[0048]Take the 10 cases of embodiment 1 label 1-10 and distribute four types of serum samples, and divide the serum / plasma samples into four categories according to the following classification methods: A jaundice, B hemolysis, C lipemia, D normal:

[0049] A. Jaundice sample: It is slightly yellower than normal serum / plasma by visual inspection, and the color can be reddish or blackish. After shaking, the tube wall has obvious orange-yellow performance, and the total bilirubin TBiL>34.2μmol / L can be judged as a jaundice sample, otherwise it is a normal serum / plasma sample;

[0050] B. Hemolyzed sample: It is slightly redder than normal serum / plasma by visual inspection, the color is darker, and the light transmittance is weakened. The hemoglobin Hb > 0.05g / L can be judged as a hemolyzed sample by colorimetry, otherwise it is a normal serum / pla...

Embodiment 3

[0057] The processing method of hepatitis C virus nucleic acid detection sample of the present invention, the steps are as follows:

[0058] 1. Take the 10 examples of the four types of serum samples classified in Example 2 with labels 1-10;

[0059] 2. Before the experiment, 10 samples were centrifuged at 12,000rpm in an Eppendorf high-speed refrigerated centrifuge for 10 minutes. Absorb the supernatant from jaundice samples 1-4 and hemolysis samples 5 and 6. Discard the upper layer of fat from lipid blood samples 7-9. Position serum, sample No. 10 was not processed;

[0060] 3, with embodiment 1 step 2-6;

[0061] 4. After 3 minutes, insert the tip into the bottom of the centrifuge tube, slowly suck out the liquid from the bottom and discard it, centrifuge for 3 seconds and place the centrifuge tube on the magnetic rack again; after standing for 1 minute, suck out the residual liquid at the bottom of the tube completely Throw it away and wait for inspection on the machine....

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Abstract

The invention discloses a hepatitis C virus nucleic acid detection sample treatment method, which belongs to the technical field of in vitro detection. The method comprises the following steps: S1, dividing serum / plasma samples into A jaundice, B hemolysis, C lipid blood and D normality; S2, respectively carrying out high-speed centrifugation on the A jaundice sample, the B hemolysis sample and the C lipid blood sample at 12000-15000rpm for 5-10min, and not treating the normal sample; and S3, respectively absorbing supernatant from the A jaundice and B hemolysis samples to obtain treated serum / plasma samples; and discarding upper suspended matters in the C lipid blood sample and sucking serum in the middle position to obtain a treated serum / plasma sample. According to the method, the classified samples are subjected to high-speed centrifugation, and compared with untreated samples, experiment inhibition caused by jaundice, hemolysis and lipid blood of the samples is avoided, the experiment success rate is guaranteed, false negative results are avoided, and the accuracy of detection results is improved.

Description

technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a method for processing hepatitis C virus nucleic acid detection samples. Background technique [0002] Hepatitis C is a disease mainly transmitted by blood. According to the statistics of the World Health Organization, the global HCV infection rate is about 3%. It is estimated that about 180 million people are infected with HCV, and there are about 35,000 new hepatitis C cases every year. Chronic infection of hepatitis C virus (Hepatitis C Vrius) can lead to chronic inflammation, necrosis and fibrosis of the liver. social and public health issues. Therefore, the detection of HCV nucleic acid can well assist the treatment of HCV. [0003] At present, kits are mainly used to detect hepatitis C virus, such as the hepatitis C virus nucleic acid detection kit produced by Hunan Shengxiang Biotechnology Co., Ltd. The sample needs to be pre-treated before the detection. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6806
CPCC12Q1/707C12Q1/6806C12Q2523/32Y02A50/30
Inventor 朱爱丽张馨月周婷婷王双阁许友强李雨艳
Owner JILIN JINYU MEDICAL SCI INSPECTION CO LTD
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