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Construction method and identification method for fingerprint spectrum of stemona tuberosa

A technology of leaves and fingerprints, applied in the field of medicine, can solve problems such as difficult to accurately judge, and achieve the effect of ensuring the variety of medicinal materials and the quality of drugs

Active Publication Date: 2020-04-14
HARBIN KANGLONG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to judge accurately when certain products need to be used accurately

Method used

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  • Construction method and identification method for fingerprint spectrum of stemona tuberosa
  • Construction method and identification method for fingerprint spectrum of stemona tuberosa
  • Construction method and identification method for fingerprint spectrum of stemona tuberosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The construction of embodiment 1 fingerprint

[0036] 1. Preparation of reference substance solution: Accurately weigh an appropriate amount of chlorogenic acid reference substance, add 30% methanol to make a solution containing 0.025mg of chlorogenic acid per 1mL, and obtain it;

[0037] 2. Preparation of the test solution: Take 1g of the Ababa leaf sample, decoct it with water, keep it slightly boiled, and at the same time concentrate the volume to about 20-30mL, cool to room temperature, filter, shake and extract 3 times with water-saturated n-butanol , 20mL each time, combine the extracts, evaporate to dryness, add an appropriate amount of 30% methanol to dissolve, quantitatively transfer to a 5mL measuring bottle, add 30% methanol to constant volume, shake well, and obtain;

[0038] 3. Chromatographic conditions: using Halo R 5C18 chromatographic column (column length is 250mm, inner diameter is 4.6mm, particle size is 5μm, manufacturer is Waters), octadecylsilan...

Embodiment 2

[0042] The construction of embodiment 2 standard fingerprints

[0043] Using the Chinese Academy of Food and Drug Control, batch number: 121221-201003 of the Abacifolia sample as the test sample, according to the fingerprints produced by the aforementioned construction method, the software generates a spectrum representing the average state, that is, the Abacifolia Standard fingerprints (such as figure 1 shown).

[0044] The standard fingerprint spectrum of the leaves contains 11 characteristic peaks.

Embodiment 3

[0045] The verification of embodiment 3 standard fingerprints

[0046] For the 9 batches of Baibu products selected from the market, and the loquat leaves that are easy to be used to replace Baibu, the fingerprints were constructed according to the method described in Example 1, and the obtained samples were processed by the Chinese medicine chromatographic fingerprint similarity evaluation software. The fingerprint spectrum and the standard fingerprint spectrum of embodiment 2 gained are analyzed.

[0047] serial number sample place of origin source 1 Hyakube Guangxi user post 2 Hyakube Guangxi Wang Yaya North Street, Anguo Medicine City, Baoding City, Hebei Province 13780426848 3 Hyakube Yunnan Bozhou Zhongyitang Traditional Chinese Medicine Sales Co., Ltd. Junjunlaitao 01 4 Hyakube Guangxi Anguo Pharmaceutical Source Trading Co., Ltd. 5 Hyakube Guangxi user post 6 Hyakube Guangxi user post 7 Hy...

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Abstract

The invention relates to the field of medicines, and particularly discloses a construction method for a fingerprint spectrum of stemona tuberosa. The construction method comprises the following steps:preparing a reference substance solution and a test solution by using 30% methanol under the chromatographic conditions of a HaloR 5 C18 chromatographic column (the column length is 250mm, the innerdiameter is 4.6 mm and the particle size is 5mu m), with a mobile phase of a mixed solution of acetonitrile-0. 4% phosphoric acid solution in a ratio of (3-40): (97-60), at the flow rate is 0.5-1.5 mL / min, in the detection wavelength from 254 nm to 325 nm, and at the column temperature is 30-40 DEG C, and the number of theoretical plates being not less than 50000 calculated according to a chlorogenic acid peak; and precisely sucking 5-20 [mu] L of the reference substance solution and 5-20 [mu] L of the test solution respectively, injecting the solutions into a liquid chromatograph, and recording chromatographic peaks for 4-30 min to obtain the fingerprint spectrum of stemona tuberosa. By utilizing the fingerprint spectrum and the construction method thereof provided by the invention, the unrigorous conditions such as secondary satisfaction, reduced feeding, change of processing and production processes and the like can be avoided, so that the possibility of producing inferior medicinesis cut off.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for constructing and identifying the fingerprints of the leaves. Background technique [0002] The Chinese Pharmacopoeia (2015 edition) contains the Erecta, the opposite leaf and the creeping Bubu under the item of a Zhongbaibu, and all three kinds can be used as a Baibu. The Pharmacopoeia only describes the subtle differences in appearance and fiber characteristics of the medicinal parts of the three kinds of medicinal parts under the item of traits and identification. In actual use, the professionalism of the users is extremely high. It is difficult to judge accurately when certain products need to be used accurately. [0003] Therefore, there is an urgent need to develop a method for specific identification of Phyllostachys praecox, and to construct a clear, non-interfering fingerprint that can identify various important active ingredients and ratios in Phyllostachys praecox,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 穆滨尹燕杰惠建梅艾春林翟斌
Owner HARBIN KANGLONG PHARM CO LTD
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