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Application of human cryptochrome protein I (hCRY1) in preparation of anti-ultraviolet radiation preparation

A technology of cryptochrome and pigment protein, which is applied in the application field of preparations, and can solve the problems of non-broad-spectrum absorption, toxic side effects, opacity, etc.

Active Publication Date: 2020-04-17
GUANGZHOU DOUBLLE BIOPRODUCT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to develop safe, environmentally friendly, non-toxic sunscreen sunscreens, researchers have conducted a lot of research. However, the current sunscreens have the characteristics of toxic side effects and non-broad-spectrum absorption, poor shading effect and opacity. Therefore, it is urgent to develop a preparation process with simple, safe, light-transmitting, water-soluble and broad-spectrum absorption properties.

Method used

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  • Application of human cryptochrome protein I (hCRY1) in preparation of anti-ultraviolet radiation preparation
  • Application of human cryptochrome protein I (hCRY1) in preparation of anti-ultraviolet radiation preparation
  • Application of human cryptochrome protein I (hCRY1) in preparation of anti-ultraviolet radiation preparation

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Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Cell experiment

[0030] Source of raw materials: UVA ultraviolet lamp (Guanya Optoelectronics, peak 360nm), UVB ultraviolet lamp (Guanya Optoelectronics, peak 320nm), UVC ultraviolet lamp (Beijing Sitong, peak 257nm), UV illuminance meter (Taiwan Cenchi), enzyme label instrument (Bio-Rad), fluorescent quantitative PCR instrument (Stratagene MX 3000P), hCRY1 recombinant human cryptochrome protein solution (Dabo Bio, Escherichia coli expression and fermentation purification), CCK-8 kit (Shanghai Yisheng Bio), 6 - 8-week-old SD rats (Guangzhou Youdi Biological), Anti-MMP-1 antibody (Bioss), SPF30 sunscreen (purchased from Mekaina), SYBR Green MIX (Shanghai Yisheng Biological).

[0031] Preparation:

[0032] (1) Cell culture: the HaCaT cells were revived and cultured in DMEM medium containing 10% FBS, continuously passaged, and the 3rd to 10th passage cells were taken for experiments.

[0033] (2) Preparation of the UV irradiation box: use a polystyrene foam bo...

Embodiment 2

[0062] Embodiment 2 animal experiments

[0063] Source of raw materials: Experimental animals: 30 SD rats (purchased from Guangzhou Youdi Biology), MMP-1 antibody (purchased from Bioss).

[0064] QPCR reagents (purchased from YEASEN): TRIZOL, reverse transcriptase, SYBR MIX, MMP-1, MMP-3, GAPDH real-time quantitative PCR primers.

[0065] Specimen preparation and immunohistochemistry: chloral hydrate, paraformaldehyde, paraffin, dewaxing agent, mounting medium, glass slide, absolute ethanol, HE staining kit, immunohistochemical secondary antibody kit, immunohistochemical kit .

[0066] UVA ultraviolet lamp 15W (Guanya Optoelectronics, wave peak 360nm), UVB ultraviolet lamp 15W (Guanya Optoelectronics, wave peak 320nm), dimmer, UV illuminance meter (Taiwan Cenchi), rat holder, electric razor, QPCR instrument (available from STRATAGENE).

[0067] A human cryptochrome protein I (hCRY1) emulsion, the emulsion comprises the following raw materials: water, glycerin, macrogol-100 ...

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Abstract

The invention discloses an application of human cryptochrome protein I (hCRY1) in preparation of an anti-ultraviolet radiation preparation, which belongs to the field of biopharmaceutical processes. Acell experiment and an animal experiment are respectively carried out, through tissue form staining and immunohistochemical staining, skin light aging change and gene expression difference on an animal model are revealed by the associated gene mRNA expression level, and then whether hCRY1 has an ultraviolet protection function or not is determined. The hCRY1 is used as a potential novel biological ultraviolet opacifier in the research, compared with a physical opacifier which is lightproof and a chemical opacifier which has the characteristics of toxic and side effects and non-broad-spectrumabsorption, the hCRY1 has the characteristics of safety, light transmission, water solubility and broad-spectrum absorption of 100-400nm ultraviolet rays.

Description

technical field [0001] The invention belongs to the field of biopharmaceutical technology, and in particular relates to the application of human cryptochrome protein I (hCRY1) in the preparation of anti-ultraviolet radiation preparations. Background technique [0002] At present, sunscreen products on the market are mainly divided into physical sunscreens and chemical sunscreens. Physical sunscreens usually reflect or scatter ultraviolet and visible light. Commonly used substances include talcum powder, zinc oxide, titanium dioxide, magnesium oxide, and kaolin. Chemical sunscreens usually absorb UV light and behave differently depending on the wavelength of UV light they absorb. For example, sunscreens that absorb long-wave ultraviolet rays (wavelength range 320-400nm) include benzophenone, 4-tert-butyl-4-methoxymethane, and 4-isopropyldiphenylmethane, etc.; absorb medium-wave ultraviolet rays (wavelength range 290-320nm) opacifiers include p-aminobenzoic acid and its deri...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K8/64A61P17/16A61Q17/04
CPCA61K8/64A61K38/1709A61Q17/04A61P17/16
Inventor 黄文林周晓鸿
Owner GUANGZHOU DOUBLLE BIOPRODUCT CO LTD
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