Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lateral flow test strip for serum marker luminescence detection based on near-infrared excitation and emission as well as preparation method and application method of lateral flow test strip

A technology for luminescent detection and markers, applied in the field of immunological detection, can solve the problems of high interference, background signal of light damage detection, etc.

Active Publication Date: 2020-04-21
HARBIN INST OF TECH
View PDF13 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the technical problems that existing nanomaterials used for biological detection have photodamage to living organisms and high background signal interference, and provide a lateral flow detection method based on near-infrared excitation and emission for serum marker luminescence detection. Test strips and methods of making and using the same

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lateral flow test strip for serum marker luminescence detection based on near-infrared excitation and emission as well as preparation method and application method of lateral flow test strip

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0057] Specific Embodiment 1: The lateral flow test strip based on near-infrared excitation and emission detection of serum marker luminescence in this embodiment is composed of a polyvinyl chloride support backplane 1, a detection pad 2, a binding pad 3, a sample pad 4 and an absorption pad 5 composition;

[0058] Wherein the detection pad 2 is composed of a nitrocellulose membrane 2-1 and a detection line 2-2 and a control line 2-3 drawn in parallel on the nitrocellulose membrane; the detection line 2-2 is a serum marker with a concentration of 2 mg / mL The line drawn by the antibody has a line width of 1 mm, and the dropping volume is 1 μL / cm; the control line 2-3 is a line drawn with an IgG antibody solution with a concentration of 2 mg / mL, and the line width is 1 mm, and the dropping volume is 1 μL / cm. is 1 μL / cm;

[0059] Conjugation pad 3 is loaded with down-converting α-NaYF that binds PCT antibody 4 :10%Yb,30%Nd@CaF 2 Nanocrystal probes and mouse IgG-bound down-conv...

specific Embodiment approach 2

[0084] Specific implementation mode two: the difference between this implementation mode and specific implementation mode one is:

[0085] Ⅰ. The product part is different: the binding pad 3 is the down-conversion α-NaGdF4 loaded with PCT antibody: 10% Yb, 30% Nd@CaF 2 Nanocrystalline probe and mouse IgG-bound downconverting α-NaGdF4:10%Yb,30%Nd@CaF 2 Nanocrystalline Probes.

[0086] Ⅱ. The preparation method is partially different:

[0087] 1. Preparation of nuclear α-NaGdF 4 :Yb, Nd, wherein α-NaGdF4:Yb, the doping mole percentage of Yb element in Nd is 10%, and the doping mole percentage of Nd element is 30%, recorded as α-NaGdF4:10%Yb,30%Nd:

[0088] a. According to α-NaGdF 4 : 10% Yb, 30% Nd stoichiometric ratio and Yb and Nd doping concentration Weigh 0.0181g of Gd 2 o 3 , 0.0197g of Yb 2 o 3 , 0.0505g of Nd 2 o 3 and 2mmol of sodium trifluoroacetate;

[0089] b. Set Gd 2 o 3 , Yb 2 o 3 and Nd 2 o 3 Dissolve in 10 mL of 50% by volume trifluoroacetic acid...

specific Embodiment approach 3

[0091] Specific implementation mode three: the difference between this implementation mode and specific implementation mode one is:

[0092] Ⅰ. The product part is different: the binding pad 3 is the down-converting α-NaLuF4 loaded with PCT antibody: 10% Yb, 30% Nd@CaF 2 Nanocrystalline probe and mouse IgG-bound downconverting α-NaLuF4:10%Yb,30%Nd@CaF 2 Nanocrystalline Probes.

[0093] Ⅱ. The preparation method is partially different:

[0094] 1. Preparation of nuclear α-NaLuF 4 :Yb, Nd, wherein α-NaLuF4:Yb, the doping mole percentage of Yb element in Nd is 10%, and the doping mole percentage of Nd element is 30%, recorded as α-NaLuF4:10%Yb,30%Nd:

[0095] a. According to α-NaLuF 4 : 10% Yb, 30% Nd stoichiometric ratio and Yb and Nd doping concentration Weigh 0.0199g of Lu 2 o 3 , 0.0197g of Yb 2 o 3 , 0.0505g of Nd 2 o 3 and 2mmol of sodium trifluoroacetate;

[0096] b. Add Lu 2 o 3 , Yb 2 o 3 and Nd 2 o 3 Dissolve in 10 mL of 50% by volume trifluoroacetic ac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lateral flow test strip for serum marker luminescence detection based on near-infrared excitation and emission as well as a preparation method and an application method of the lateral flow test strip, which belongs to the field of immunological detection and aims to solve the technical problems that an existing nano material for biological detection has optical damage toorganisms and has high detection background signal interference. The lateral flow test strip consists of a polyvinyl chloride supporting back plate, a detection pad, a combination pad, a sample pad and an absorption pad, wherein the combination pad is a down-conversion [alpha]-NaLnF4:Nd,Yb@CaF2 / NaLnF4 nanocrystalline probe loaded with a combination antibody, and a down-conversion [alpha]-NaLnF4:Nd,Yb@CaF2 / NaLnF4 nanocrystalline probe combined with mouse IgG. The lateral flow test strip provided by the invention has a wide detection linear range and strong applicability, has low requirements onthe volume of a to-be-detected sample, short in required detection time, capable of realizing bedside detection and high in flexibility; and the lateral flow test strip does not need professional personnel to operate and can be used for medical detection.

Description

technical field [0001] The invention belongs to the field of immunological detection. In particular, it relates to a lateral flow test strip for detecting serum markers and a method for preparing and using the same. Background technique [0002] In recent years, with the continuous development of mobile medical care and home medical care, researchers are constantly seeking new types of luminescent materials as optical probes to obtain higher sensitivity and stability testing methods. Nowadays, people can detect various disease markers in serum, including myocardial infarction markers (CTNI and MYO); bacterial and viral infection markers (CRP, SAA and PCT); diabetes markers (glycosylated hemoglobin); Pro-infectious disease markers (HIV, syphilis, hepatitis B and C), etc. Among them, the detection method based on gold nanoparticles has developed rapidly, and has been rapidly applied in the fields of pregnancy detection and infection detection. However, the detection method ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/63G01N21/359
CPCG01N21/63G01N21/359
Inventor 陈冠英曹宁韩晓瑞
Owner HARBIN INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com