Lateral flow test strip for serum marker luminescence detection based on near-infrared excitation and emission as well as preparation method and application method of lateral flow test strip
A technology for luminescent detection and markers, applied in the field of immunological detection, can solve the problems of high interference, background signal of light damage detection, etc.
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specific Embodiment approach 1
[0057] Specific Embodiment 1: The lateral flow test strip based on near-infrared excitation and emission detection of serum marker luminescence in this embodiment is composed of a polyvinyl chloride support backplane 1, a detection pad 2, a binding pad 3, a sample pad 4 and an absorption pad 5 composition;
[0058] Wherein the detection pad 2 is composed of a nitrocellulose membrane 2-1 and a detection line 2-2 and a control line 2-3 drawn in parallel on the nitrocellulose membrane; the detection line 2-2 is a serum marker with a concentration of 2 mg / mL The line drawn by the antibody has a line width of 1 mm, and the dropping volume is 1 μL / cm; the control line 2-3 is a line drawn with an IgG antibody solution with a concentration of 2 mg / mL, and the line width is 1 mm, and the dropping volume is 1 μL / cm. is 1 μL / cm;
[0059] Conjugation pad 3 is loaded with down-converting α-NaYF that binds PCT antibody 4 :10%Yb,30%Nd@CaF 2 Nanocrystal probes and mouse IgG-bound down-conv...
specific Embodiment approach 2
[0084] Specific implementation mode two: the difference between this implementation mode and specific implementation mode one is:
[0085] Ⅰ. The product part is different: the binding pad 3 is the down-conversion α-NaGdF4 loaded with PCT antibody: 10% Yb, 30% Nd@CaF 2 Nanocrystalline probe and mouse IgG-bound downconverting α-NaGdF4:10%Yb,30%Nd@CaF 2 Nanocrystalline Probes.
[0086] Ⅱ. The preparation method is partially different:
[0087] 1. Preparation of nuclear α-NaGdF 4 :Yb, Nd, wherein α-NaGdF4:Yb, the doping mole percentage of Yb element in Nd is 10%, and the doping mole percentage of Nd element is 30%, recorded as α-NaGdF4:10%Yb,30%Nd:
[0088] a. According to α-NaGdF 4 : 10% Yb, 30% Nd stoichiometric ratio and Yb and Nd doping concentration Weigh 0.0181g of Gd 2 o 3 , 0.0197g of Yb 2 o 3 , 0.0505g of Nd 2 o 3 and 2mmol of sodium trifluoroacetate;
[0089] b. Set Gd 2 o 3 , Yb 2 o 3 and Nd 2 o 3 Dissolve in 10 mL of 50% by volume trifluoroacetic acid...
specific Embodiment approach 3
[0091] Specific implementation mode three: the difference between this implementation mode and specific implementation mode one is:
[0092] Ⅰ. The product part is different: the binding pad 3 is the down-converting α-NaLuF4 loaded with PCT antibody: 10% Yb, 30% Nd@CaF 2 Nanocrystalline probe and mouse IgG-bound downconverting α-NaLuF4:10%Yb,30%Nd@CaF 2 Nanocrystalline Probes.
[0093] Ⅱ. The preparation method is partially different:
[0094] 1. Preparation of nuclear α-NaLuF 4 :Yb, Nd, wherein α-NaLuF4:Yb, the doping mole percentage of Yb element in Nd is 10%, and the doping mole percentage of Nd element is 30%, recorded as α-NaLuF4:10%Yb,30%Nd:
[0095] a. According to α-NaLuF 4 : 10% Yb, 30% Nd stoichiometric ratio and Yb and Nd doping concentration Weigh 0.0199g of Lu 2 o 3 , 0.0197g of Yb 2 o 3 , 0.0505g of Nd 2 o 3 and 2mmol of sodium trifluoroacetate;
[0096] b. Add Lu 2 o 3 , Yb 2 o 3 and Nd 2 o 3 Dissolve in 10 mL of 50% by volume trifluoroacetic ac...
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