Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining follicle-stimulating hormone

A follicle-stimulating hormone and antibody detection technology, applied in measuring devices, biological testing, instruments, etc., can solve the problems of large-scale measurement uncertainty, high detection cost, high price, etc., to save reaction time, rapid quantitative detection, sample less effect

Inactive Publication Date: 2020-04-21
WUHAN UNIV
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, RIA also has some shortcomings in the determination of FSH concentration: such as relatively low sensitivity, uncertainty in large-scale measurements, small calibration range (2-60ng / mL), and a single sample requires a volume of 60 μL
At the same time, ELISA detection requires a special spectrometer for absorbance measurement, which is relatively expensive and brings a large detection cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining follicle-stimulating hormone
  • Method for determining follicle-stimulating hormone
  • Method for determining follicle-stimulating hormone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Such as figure 1 and figure 2 As shown, the microfluidic chip of the present invention includes: a first inlet channel 1, a second inlet channel 2, a third inlet channel 3, a plurality of inlet branches 4, a reaction part 5, and an outlet channel 6 And waste liquid collection part 7.

[0037] A pipette gun 8 can be used to inject the detection sample into the reaction part 5 through the first inlet flow channel 1 (any inlet flow channel is acceptable), and the PBS buffer can enter and wash the reaction part 5 through the second inlet flow channel 2, and the staining The final detection antibody can be injected into the reaction part 5 through the third inlet flow channel 3 , and the waste liquid generated after washing flows into the waste liquid collection part 7 connected thereto through the outlet flow channel 6 . In this embodiment, the first inlet channel 1 , the second inlet channel 2 and the third inlet channel 3 have a width of 200 μm and a height of 100 μm, ...

Embodiment 2

[0041] The capture antibody used in this example is Anti-FSH R antibody (Hytext, Finland), the detection antibody is Anti-h FSH6602SP-5 (Medix Biochemica, Finland), the dye is DY647P4 fluorescent dye (Dyomics, Germany), and the detection sample For mouse serum samples.

[0042] A method for measuring follicle-stimulating hormone, comprising the steps of:

[0043] (1) Making a microfluidic chip;

[0044] (2) Mix the hydrogel with the capture antibody and solidify it on the reaction part of the microfluidic chip, and rinse it with PBS multiple times to remove uncured raw materials;

[0045] (3) Add 10 μL of the test sample and incubate for 15 minutes to make the FSH in the test sample bind to the capture antibody, and wash with PBS several times to remove the unbound test sample;

[0046] (4) Add the stained detection antibody and incubate for 15 minutes to make the stained detection antibody combine with the FSH-antibody conjugate to emit fluorescence, and wash with PBS sever...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Widthaaaaaaaaaa
Heightaaaaaaaaaa
Radiusaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of biological detection methods, in particular to a method for determining follicle-stimulating hormone. The method comprises the following steps of: (1) manufacturing a micro-fluidic chip; (2) mixing hydrogel with a capture antibody, and curing the mixture in the micro-fluidic chip; (3) adding the micro-fluidic chip into a detection sample and incubating the sample, so that FSH in the detection sample is combined with the capture antibody; (4) adding a dyed detection antibody and incubating the sample, so that the dyed detection antibody is combined with the FSH-antibody conjugate to emit fluorescence; and (5) photographing the detected hydrogel, analyzing the fluorescence intensity of the photographed picture, and calculating the content of follicle-stimulating hormone in the detected sample. According to the method for determining the follicle-stimulating hormone, a novel detection mode is generated through combination of a microfluidic technology and ELISA, the required sample amount is small, and one-step, rapid and quantitative detection of the FSH content in the detection sample is realized.

Description

technical field [0001] The invention relates to the technical field of biological detection methods, in particular to a method for measuring follicle-stimulating hormone. Background technique [0002] Follicle-stimulating hormone (FSH) is a gonadotropin released by the anterior pituitary gland. It plays a key role in female reproductive development and homeostasis by stimulating germ cell maturation and stimulating follicle growth. FSH concentration in blood or serum is an important indicator of the function of the hypothalamic-pituitary-gonadal axis (HPG axis), which is applicable to the stages of puberty and estrus in mammals, as well as the stages of the human menstrual cycle. During each estrus, the concentration of FSH in the circulating blood changes drastically within a short period of time. For rapid monitoring of FSH concentrations during such events, a fast, reliable, sensitive on-site measurement method with low sample consumption is highly desired. [0003] Cu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/76G01N33/50
CPCG01N33/50G01N33/76G01N2333/59
Inventor 杨奕朱娇梦
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com