Electrochemical aptamer sensor for detecting Alzheimer disease marker, manufacturing method and application thereof
An aptamer sensor and Alzheimer's disease technology, applied in the field of electrochemical biosensing, can solve the problems of cumbersome detection methods, high cost, high detection limit, etc., achieve large electron transmission capacity, reduce cost, and increase current Effect
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Embodiment 1
[0037] A method for preparing an electrochemical aptasensor for detecting Alzheimer's disease markers, the steps are as follows:
[0038] 1. The preparation of gold and platinum co-deposited AuPt nanoflower structure, the specific steps are as follows:
[0039] Prepare the AuPt nanoflower structure by electrodeposition: prepare a sulfuric acid solution (0.5M) of chloroauric acid (10mg / mL) and chloroplatinic acid (10mg / mL), mix them in equal volumes, use a three-electrode system, conductive glass (ITO) as the working electrode, Ag / AgCl as the reference electrode, and the platinum sheet electrode as the auxiliary electrode, with a voltage of -0.2V, deposited for 300s, and the gold-platinum nanoflower structure can be grown on the surface of the ITO, and its morphology is shown in figure 1 ,Depend on figure 1 It can be seen that the overall size of the nanoflower structure is about 80-800 nm; according to the energy spectrum analysis diagram of the AuPt nanoflower structure (att...
Embodiment 2
[0049] The detection of Alzheimer's disease marker Aβ oligomers, the steps are as follows:
[0050] (1) The electrochemical workstation was used to test with a three-electrode system. The Ag / AgCl electrode was used as the reference electrode, the platinum electrode was used as the auxiliary electrode, and the prepared sensor was used as the working electrode. Test in buffer;
[0051] (2) When using square wave voltammetry (DPV) to detect different Aβ oligomer concentrations, the input voltage range is 0.0-0.4V;
[0052] (3) Record the change value of DPV peak current.
Embodiment 3
[0054] Performance Analysis of Prepared Sensors
[0055] In order to evaluate the performance of the prepared electrochemical aptasensor, different concentrations of Aβ oligomer standard prepared in phosphate buffer solution with pH 7.4 (concentrations were 0.05, 0.1, 1, 10, 100, 1000, 10000 pg / mL) to prepare different aptasensors. The electrochemical workstation was used to test with a three-electrode system, the Ag / AgCl electrode was used as the reference electrode, the platinum electrode was used as the auxiliary electrode, and the prepared sensor was used as the working electrode. The test uses the DPV method to test the peak current change values corresponding to different concentrations of standard products at an input voltage of 0.0-0.4V, and draw a standard curve, (such as image 3 As shown, ΔI= 12.008 LgC +153.13, R 2 =0.9928, C is the concentration of Aβ oligomers, ΔI is the difference of peak current) such as image 3 shown. The detection limit of the sensor o...
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