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Multiple immune molecule detection method and kit

A technology of immune molecules and detection methods, which is applied in the field of multiple immune molecular detection methods and kits, can solve the problems of low electrochemical stability and low electrocatalytic activity of oxygen reduction, and achieve sample size saving, efficiency improvement, and high application value effect

Pending Publication Date: 2020-04-24
COLORTECH SUZHOU BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problems of low electrocatalytic activity for oxygen reduction and low electrochemical stability of current silver-based nanocatalysts

Method used

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  • Multiple immune molecule detection method and kit
  • Multiple immune molecule detection method and kit

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Experimental program
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Embodiment 1

[0048] Simultaneous detection of IL-2 and IL6 cytokines with high sensitivity assay

[0049] 1. Synthesis of two fluorescently encoded microspheres: styrene monomer, polymethyl methacrylate, initiator, crosslinking agent, and acrylic acid oligomer were mixed in chloroform as a polymer solution. Take two 4.5mL polymer solutions and record them as reaction solution 1 and reaction solution 2. Add 0.5mL rhodamine, 0.5mL fluorescein and 90mg nano-magnetic particles to reaction solution 1, and add 0.5mL rhodamine and 0.5mL fluorescein to reaction solution 2. and 90mg nano-magnetic particles. Afterwards, the reaction solution 1 and the reaction solution 2 were respectively placed in two reactors with 300mL deionized water and a surfactant, and the reaction solution 1 and the reaction solution 2 were uniformly dispersed into For microemulsion droplets with a size of about 10 microns, an initiator is added to the solution and heated for polymerization and cross-linking reaction. After...

Embodiment 2

[0058] Simultaneous detection of two cytokines, IL10 and IFNr, with high sensitivity

[0059] 1. Synthesis of two fluorescently encoded microspheres: styrene monomer, polymethyl methacrylate, initiator, crosslinking agent, and acrylic acid oligomer were mixed in chloroform as a polymer solution. Two 4.5mL polymer solutions were taken as reaction solution 1 and reaction solution 2, 0.5mL rhodamine, 0.5mL fluorescein and 90mg nano-magnetic particles were added to 1, and 0.5mL rhodamine, 0.5mL fluorescein and 90mg nano-magnetic particles were added to 2. Afterwards, 1 and 2 were placed in two reactors with 300mL deionized water and surfactants respectively. Taking 1 as an example, the 1 solution was uniformly dispersed into a 10-micron liquid under the conditions of stirring, ultrasonication, and surface force adjustment. Drops, add an initiator to the solution and heat to carry out polymerization and cross-linking reaction. After 24 hours, the chloroform in each droplet slowly d...

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Abstract

The invention discloses a multiple immune molecule detection method. The method comprises the following steps: obtaining an encoding microsphere of which the surface is connected with a capture molecule; capturing a target immune molecule through the capture molecule and adding an enzyme-labeled reagent to form an enzyme-labeled immune sandwich compound on the surface of the encoding microsphere;driving the encoding microsphere of which the surface is modified with the immune sandwich compound into micro-pores of a micro-well plate, and sealing the encoding microsphere; and after a preset period of time, exciting the micro-well plate by light to detect the micro-well plate. Protein detection reaches the level of fg / ml, and the sensitivity is 1000 times higher than that of a traditional ELISA method. The method has great advantages especially for single molecule detection, and has broad application prospects in the aspects of life science, in-vitro diagnosis, accompanying diagnosis, blood screening, drug research and development and the like.

Description

technical field [0001] The invention belongs to the field of immune molecular detection, and in particular relates to a multiple immune molecular detection method and a kit. Background technique [0002] The traditional ELISA reaction system is relatively large (100 μl), requiring millions of molecules to generate tens of millions of fluorophore signals before they can be detected by the microplate reader. Therefore, the traditional ELISA method can only detect the picomolar range (10pg / ml ) above the signal, the simulation algorithm is used. This method has reached the limit in modern science and is far from meeting clinical requirements. For example, some low-abundance protein molecules in peripheral blood are of great significance to the guidance of clinical diagnosis and treatment, but the sensitivity of traditional ELISA cannot detect these molecules, let alone achieve sensitive monitoring. ELISA can only detect one indicator in one experiment per sample. However, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/535G01N33/543
CPCG01N33/533G01N33/535G01N33/54326G01N33/54346
Inventor 陈忠磊张敏超程鹏
Owner COLORTECH SUZHOU BIOTECHNOLOGY CO LTD