A kind of extraction method of quinoa straw vegetable protein

Abstract

The invention discloses a method for extracting plant protein from quinoa stalks, which belongs to the technical field of separation and extraction of plant components. The method comprises the following steps: harvesting quinoa stalks, mixing liquid, beating, ultrasonic treatment, adjusting pH, adding enzymes, enzymatic hydrolysis, Enzyme inactivation, filtration, centrifugation, drying, and gel column chromatography; the plant protein extraction method of the present invention scientifically and rationally improves the protein yield, reasonably controls the reaction conditions by analyzing the protein properties, and protects the protein quality from the source. Extraction of plant protein by enzymatic method can protect the protein structure from damage, mild reaction conditions, nutrients are basically not damaged, and the solid-liquid ratio of the reaction is small, which not only saves solvent consumption, but also improves the final solution. The content of solids is reduced, thereby reducing the energy consumption of removing water in the solution, increasing the protein extraction rate and improving the protein purity, creating conditions for industrial production.

Description

technical field [0001] The invention relates to the technical field of separation and extraction of plant components, in particular to a method for extracting plant protein from quinoa stalks. Background technique [0002] Quinoa, also known as South American quinoa, Indian wheat, quinoa and quinoa, is an annual herb belonging to the family Chenopodiaceae. Quinoa is native to the Andes in Bolivia, Chile, and Peru. It has been gradually promoted as a health product in North America and Europe. In recent years, it has been widely planted in Shanxi, Jilin, Qinghai, Gansu, and Hebei in my country. Quinoa is the first choice of space food for American astronauts and the only complete nutritional food recommended by the Food and Agriculture Organization of the United Nations. It is an internationally recognized complete nutritional and perfect food. Quinoa is rich in comprehensive and balanced nutrients such as protein, cellulose, and minerals, and has various pharmacological eff...

AI Technical Summary

Problems solved by technology

However, many of these methods have shortcomings. Although the alkali-dissolving and acid-precipitating method is simple in process and low in cost, it is easy to cause environmental pollution if the wastewater is not properly treated, and the extracted protein is likely to contain toxic substances, and it is also prone to denaturation in the case of strong acid and strong alkali; The salt solution method will not affect the properties of the protein, and the post-treatment is simpler than other methods, but it consumes a lot of salt and is not suitable for industrial production; the organic solvent method has a higher yield, but it is more destructive to sulfur-containing amino acids and the quality of the protein. Poor, and it is easy to cause organic reagent residue; biological enzyme method has mild reaction conditions, nutrients are basically not destroyed, and the reaction solid-liquid ratio is small, which saves reagent consumption and creates conditions for industrial production.

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