Unlock instant, AI-driven research and patent intelligence for your innovation.

Vitamin B12 fermentation supplementing culture medium and feeding method

A feed medium, B12 technology, applied in the field of biopharmaceuticals, can solve the problems of weakened glycine supply and affecting the growth and synthesis of bacteria, so as to relieve substrate inhibition, improve the phenomenon of aging and emptying of bacteria, and balance the growth of bacteria in the later stage. effects on metabolism

Active Publication Date: 2020-04-28
HUBEI GUANGJI PHARMA
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can achieve better results in the stage of vigorous growth and metabolism of the bacteria, but in the middle and late stages of fermentation after 130 hours, as the growth and metabolism of the bacteria weaken, the catabolism of the bacteria to betaine weakens, and glycine and The supply of methyl groups is also weakened, which further affects the growth of bacteria and the growth of vitamin B. 12 Synthesis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vitamin B12 fermentation supplementing culture medium and feeding method
  • Vitamin B12 fermentation supplementing culture medium and feeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. First-level seed cultivation: the volume of seed liquid is 3L

[0026] Seed medium: Add 400-600g of sucrose, 120-150g of corn steep liquor, 40-60g of betaine, 3.5-5g of magnesium oxide, 20-30g of diammonium hydrogen phosphate, 0.5-1g of zinc sulfate, and Calcium 30-50g, 5-6 dimethylbenzimidazole 0.7-1.4g, ammonium sulfate 10-20g, potassium dihydrogen phosphate 10-20g. Sterilization treatment is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-124°C; sterilization time 30 minutes. Cool and keep the pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, and then under the protection of the flame, insert the cultivated Pseudomonas denitrifica mother bottle seeds into the seed medium according to the inoculation amount of 1-3%. Carry out seed culture until the OD600 of the bacteria is 11.3, the pH value is 6.08, and the culture time is 20-40 hours, and the transplanting ratio is controlled at 5...

experiment example 2

[0054] 1. First-level seed cultivation: the volume of seed liquid is 6L

[0055] Seed medium: Add 800-1200g of sucrose, 240-400g of corn steep liquor, 80-120g of betaine, 7-10g of magnesium oxide, 40-60g of diammonium hydrogen phosphate, 1-2g of zinc sulfate, and Calcium 60-100g, 5,6-dimethylbenzimidazole 1.4-3g, ammonium sulfate 20-30g, potassium dihydrogen phosphate 20-30g. Sterilization treatment is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-124°C; sterilization time 30 minutes. Cool and keep the pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, and then under the protection of the flame, insert the cultivated Pseudomonas denitrifica mother bottle seeds into the seed medium according to the inoculation amount of 1-3%. Carry out seed culture until the OD600 of the bacteria is 12.4, the pH value is 6.18, and the culture time is 20-40 hours, and the transplanting ratio is controlled at 5-1...

experiment example 3

[0082] 1. First-level seed cultivation: the volume of seed liquid is 60L

[0083] Seed medium: Add 6400-9600g of sucrose, 1920-3000g of corn steep liquor, 640-1000g of betaine, 56-100g of magnesium oxide, 320-500g of diammonium hydrogen phosphate, 8-12g of zinc sulfate, and Calcium 480-720g, 5,6-dimethylbenzimidazole 11.2-15g, ammonium sulfate 160-240g, potassium dihydrogen phosphate 160-240g. Sterilization treatment is carried out after the batching is completed. Sterilization conditions: pressure 0.10-0.14MPa; temperature 120-124°C; sterilization time 30 minutes. Cool and keep the pressure with sterile air, the pressure is controlled at 0.01-0.02MPa, and then under the protection of the flame, insert the cultivated Pseudomonas denitrifica mother bottle seeds into the seed medium according to the inoculation amount of 1-3%. Carry out seed culture until the OD600 of the bacteria is 12.6, the pH value is 6.12, and the culture time is 20-40 hours, and the transplanting ratio is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biological pharmacy, in particular to a vitamin B12 fermentation supplementing culture medium and a supplementing method, and provides a fermentation supplementing culture medium and a supplementing method capable of maintaining thallus growth and propagation in the middle and later periods of fermentation and continuously synthesizing vitamin B12. According to the invention, different supplementary materials are fed in different fermentation stages, the contents of total sugar, betaine, dissolved oxygen and glycine are controlled to be optimal in different stages, the thallus growth and metabolism relationship in the middle and later periods of fermentation is balanced, the thallus aging and emptying phenomena are fully improved, and the fermentation unit is improved; substrate inhibition, product feedback inhibition and catabolic metabolite repression are relieved; and all influences caused by massive growth of cells due to excessive one-time feeding are avoided, and the rheological property of fermentation liquor is improved.

Description

technical field [0001] The present invention relates to the technical field of biopharmaceuticals, in particular to B 12 Fermentation feed medium and feed method. Background technique [0002] Vitamin B 12 Also called cobalamin, it is the only vitamin containing metal elements. Vitamin B 12 It is a kind of vitamin produced by fermentation with Pseudomonas denitrification as the fermentation strain. Vitamin B 12 The main physiological function is to participate in the manufacture of bone marrow red blood cells to prevent pernicious anemia; to prevent brain nerves from being damaged. It is an indispensable vitamin for the healthy function of the nervous system. [0003] In the prior art, betaine needs to be supplemented in the fermentation process of using Pseudomonas denitrifica as the starting strain. Betaine can promote the secretion of metabolites on the cell surface to stimulate bacteria to produce vitamin B 12 . In addition, betaine is a methyl donor in the proc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/42C12N1/20C12R1/38
CPCC12P19/42C12N1/20
Inventor 卢正东陈情雷华夏炜铠肖其磊
Owner HUBEI GUANGJI PHARMA