Extraction method of ganoderma lucidum polysaccharide
A technology of Ganoderma lucidum polysaccharide and extraction method, which is applied in the field of extraction of Ganoderma lucidum polysaccharide, can solve the problems of excessive organic solvent, high production cost, and low extraction yield, and achieve the effect of simple production process, low production cost, and high extraction rate
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Embodiment 1
[0013] A method for extracting ganoderma polysaccharides, comprising the following steps:
[0014] (1) Raw material processing: removing impurities and cleaning the ganoderma lucidum, then soaking it in citric acid water for 24 hours, draining it for later use after soaking; wherein the concentration of the citric acid water is 1.2% (mass percentage), the ratio of ganoderma lucidum to citric acid water 4-5g: 10ml; put the Ganoderma lucidum pulp into the container, add 2-3 times the weight of distilled water, then add 0.1-0.2% high-temperature liquefied enzyme and mix well, boil for 30 minutes, cool to room temperature; then put it in 38-42℃ In a constant temperature water bath, adjust the pH to 5.5, add 0.6-0.8% ganoderma lucidum compound enzyme by weight, stir and keep warm for 100-120min to obtain saccharified syrup; the high-temperature liquefying enzyme activity is 100,000 U / ml.
[0015] (2) Separation and impurity removal: process step (2) without centrifuging the extract...
Embodiment 2
[0018] A method for extracting ganoderma polysaccharides, comprising the following steps:
[0019] (1) Raw material processing: removing impurities and cleaning the ganoderma lucidum, then soaking it in citric acid water for 25 hours, draining it for later use after soaking; wherein the concentration of the citric acid water is 1.0% (mass percentage), the ratio of ganoderma lucidum to citric acid water 4-5g: 15ml; put the Ganoderma lucidum pulp into the container, add 2-3 times the weight of distilled water, then add 0.1-0.2% high-temperature liquefied enzyme and mix well, boil for 30 minutes, cool to room temperature; then put it in 38-42℃ In a constant temperature water bath, adjust the pH to 6.0, add 0.6-0.8% ganoderma lucidum compound enzyme by weight, stir and keep warm for 100-120min to obtain saccharified syrup; the high-temperature liquefaction enzyme activity is 100,000 U / ml.
[0020] (2) Separation and impurity removal: process step (2) without centrifuging the extra...
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