A kind of nitrite detection method based on carbon nitride quantum dots

A technology of nitrite and detection method, applied in the field of fluorescent probe detection, can solve the problems of insufficient selectivity, inability to visualize, and limited application, etc.

Active Publication Date: 2022-07-15
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been reported that some fluorescence analysis methods have been used to detect nitrite. Such methods are based on quantum dots, metal cluster organic fluorophores or other materials as probes. However, these fluorescent probes have some shortcomings such as sensitivity Low, insufficient selectivity, and inability to visualize, limiting their application in practical detection

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  • A kind of nitrite detection method based on carbon nitride quantum dots
  • A kind of nitrite detection method based on carbon nitride quantum dots
  • A kind of nitrite detection method based on carbon nitride quantum dots

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Embodiment 1

[0029] An embodiment of the present invention is a method for detecting nitrite based on carbon nitride quantum dots, comprising the following steps:

[0030] 1) Preparation of green g-CNQDs by low-temperature solid-phase synthesis method: First, 0.101 g (1.68 mM) urea and 0.055 g (0.187 mM) sodium citrate were mixed in an agate mortar and ground uniformly, and then the mixed powder was transferred to Into the autoclave and heated at 180 ° C for 1 h. Finally, the solid obtained by the reaction was centrifuged with ethanol (8000 rpm, 10 min) for three times and then dispersed into water, and purified g-CNQDs were obtained after dialysis with a dialysis membrane for 24 h. The morphology of g-CNQDs was characterized by TEM and HRTEM images. like figure 1 As shown, the as-prepared g-CNQDs are spherical and have a lattice spacing of 0.213 nm.

[0031] 2) Fluorescence quenching effect of phenanthroline-ferrous ion complexes on carbon nitride quantum dots: 5mM ferrous sulfate solu...

Embodiment 2

[0041] 1) A low-temperature solid-phase synthesis method was used to prepare green fluorescent carbon nitride quantum dots. First, 0.05 g of ammonia water and 0.01 g of sodium citrate were mixed in an agate mortar and ground uniformly, and then the mixed powder was transferred to an autoclave and heated at 100 °C for 4 h. Finally, the solid obtained by the reaction was washed three times with ethanol, then centrifuged and dispersed into water, and the purified carbon nitride quantum dots were obtained after dialysis with a dialysis membrane for 20 hours.

[0042] 2) Freshly prepared 5 mM ferrous chloride and 10 mM BPS chromogenic solutions, respectively. First, according to Fe 2+ The volume ratio with the color-developing solution is 1:2, and the different amounts of Fe 2+ The solution and BPS chromogenic solution were added to 4 mL centrifuge tubes to prepare complexes of different concentrations. Then, 10 μL of carbon nitride quantum dot solution was added continuously, a...

Embodiment 3

[0047] 1) A low-temperature solid-phase synthesis method was used to prepare green fluorescent carbon nitride quantum dots. First, 1.0 g of polyethyleneimine and 0.1 g of sodium citrate were mixed in an agate mortar and ground uniformly, and then the mixed powder was transferred to an autoclave and heated at 200 °C for 0.5 h. Finally, the solid obtained by the reaction was washed three times with ethanol, then centrifuged and dispersed into water, and the purified carbon nitride quantum dots were obtained after dialysis with a dialysis membrane for 30 hours.

[0048] 2) Freshly prepared 5mM ferrous iodide solution and 10mM chromogenic solution methylene blue, respectively. First, according to Fe 2+ The volume ratio with the color-developing solution is 1:2, and the different amounts of Fe 2+ The solution and chromogenic solution were added to 4 mL centrifuge tubes to prepare complexes of different concentrations. Then, 100 μL of carbon nitride quantum dot solution was added...

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Abstract

The present invention provides a method for detecting nitrite based on carbon nitride quantum dots, comprising the following steps: 1) providing a carbon nitride quantum dot g-CNQDs that emits fluorescence at an excitation wavelength; 2) combining a color developer with Contains ferrous ion Fe 2+ 3) mixing the carbon nitride quantum dots g-CNQDs with the colored complex, the fluorescence of the carbon nitride quantum dots g-CNQDs is quenched; 4) adding sub Nitrate samples, Fe in the colored complex 2+ oxidized to Fe 3+ , the fluorescence recovery of carbon nitride quantum dots, indicating the presence of nitrite. The present invention successfully prepares green light carbon nitride quantum dots g-CNQDs. A color developing agent was introduced, and a rapid detection method of nitrite was designed through internal filtration effect and redox effect.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probe detection, and in particular relates to a method for detecting nitrite by a visualized fluorescent probe based on carbon nitride quantum dots. Background technique [0002] Nitrite ion (NO 2 - ) is a hazardous and potential contaminant to humans and is widely used as a preservative, corrosion inhibitor and fertiliser. Excessive accumulation of nitrite in the body has many adverse effects, especially in infants and children, and may lead to hyperhemoglobinemia and central nervous system birth defects. Some medical problems such as esophageal or gastric cancer are also thought to be associated with excessive intake of nitrite ions. Under acidic conditions in the stomach, nitrite can interact with amines or amides to form highly carcinogenic N-nitrosamine compounds that induce cancer, hypertension, and neurodegenerative diseases. In this context, accurate analysis of nitrite in food and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 何瑜宋功武
Owner HUBEI UNIV
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