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Method for determining ginsenoside in rat plasma

A technology of ginsenosides and blood plasma, which is applied in the detection field of ginsenosides, can solve the problems of high instrument cost, and achieve the effects of high sensitivity, good standard addition recovery and good repeatability

Inactive Publication Date: 2020-05-15
蒋蓉
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the method for studying the pharmacokinetics of ginsenosides in plasma is mainly liquid chromatography-tandem mass spectrometry, which has relatively high instrument costs, and there is no research report on the above five ginsenosides by simply using gas chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 100 μL of rat plasma, put it in a 2 mL centrifuge tube, add 200 μL methanol, vortex for 1 min, and centrifuge. Take the supernatant, put it into another clean 2 mL centrifuge tube, add 500 μL of dichloromethane, vortex for 1 min, and centrifuge. The above two centrifugation conditions were 1000 g, 3 min. The Oasis HLB column was activated sequentially with methanol, water, and dichloromethane, and the extracted supernatant was added, eluted with 3 mL of methanol, and the eluate was collected. The eluent was taken, blown with nitrogen gas to near dryness, and the residue was redissolved in methanol.

[0026] The chromatographic conditions are as follows: DB-35 MS, 30 m×0.25 mm, 0.25 μm; heating program: initial temperature is 60°C, rising to 160°C at a rate of 50°C / min, and then rising to 220°C at a rate of 20°C; Sample port temperature 250°C; carrier gas: nitrogen, purity ≥99.999%; flow rate 1.2 mL / min; injection volume 1 μL; splitless injection.

[0027] The de...

Embodiment 2

[0030] standard curve drawing

[0031] Add different volumes of ginsenoside reference solution in the centrifuge tube, after drying in a water bath, add blank plasma to form plasma samples containing different concentrations of ginsenoside, analyze using the pretreatment-analysis method of Example 1, and obtain a chromatogram Figure, record the peak area, take the peak area as the ordinate, and the concentration as the abscissa, draw the standard curve. The regression curves of ginsenosides Ra1, Rb1, Rh1, Re and Ro are as follows: y=0.057x+0.027, y=0.007x+0.215, y=0.098x-0.125, y=0.105x-0.024, y=0.0127x+ 0.114.

[0032] Method recovery

[0033] In order to verify the recovery rate of the method, the sample recovery experiment was carried out at three levels of high, medium and low respectively. The added material was then extracted and quantified. Three repetitions were performed for each of the three levels of high, middle and low. The results showed that the recoveries ...

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Abstract

The invention relates to detection of drug metabolism in blood plasma, in particular to a method for detecting five ginsenosides in rat blood plasma, which is characterized by comprising the followingsteps of precipitating protein by using methanol, extracting by using dichloromethane, purifying by using a solid-phase extraction column, and detecting Ra1, Rb1, Rh1, Re and Ro in the blood plasma by using gas chromatography. Compared with the prior art, the method has the advantages of high accuracy, high sensitivity and good repeatability, is suitable for simultaneous quantitative detection ofthe five ginsenosides in the blood plasma, and can be used for research on metabolic pathways of the ginsenosides in the blood plasma.

Description

technical field [0001] The invention relates to a method for detecting ginsenosides in rat plasma, which includes simultaneous quantitative detection of five ginsenosides, Ra1, Rb1, Rh1, Re and Ro. Background technique [0002] Ginseng has a wide range of pharmacological activities on the central nervous system, cardiovascular, endocrine, and immune systems. Ginseng saponin and its hydrolyzate in the intestinal tract are considered to be the main pharmacologically active ingredients in Asian ginseng, American ginseng, and Panax notoginseng. However, their mechanism of action is still unclear. It is very clear that the evaluation of the exposure level of the body to the components of saponins after taking ginseng preparations will help to uncover the material basis of its medicinal effects. [0003] In recent years, the pharmacokinetics of traditional Chinese medicine has been widely used in the interpretation of the material basis and mechanism of action of traditional Chine...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/68
CPCG01N30/02G01N30/06G01N30/68G01N2030/8822
Inventor 蒋蓉
Owner 蒋蓉
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