Preparation method of astaxanthin-containing biological antibacterial composite membrane for inhibiting spoilage bacteria of penaeus vannamei boone

A technology of astaxanthin and composite film, which is applied in food ingredients as antimicrobial preservation, food science, application, etc., to achieve the effects of improving antibacterial activity, easy operation, inhibiting growth and in vitro free radical oxidation

Active Publication Date: 2020-05-19
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on the application of astaxanthin in chitosan gelatin base film.

Method used

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  • Preparation method of astaxanthin-containing biological antibacterial composite membrane for inhibiting spoilage bacteria of penaeus vannamei boone
  • Preparation method of astaxanthin-containing biological antibacterial composite membrane for inhibiting spoilage bacteria of penaeus vannamei boone
  • Preparation method of astaxanthin-containing biological antibacterial composite membrane for inhibiting spoilage bacteria of penaeus vannamei boone

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preparation example Construction

[0039] A method for preparing an astaxanthin-containing biological antibacterial composite film for inhibiting spoilage bacteria of Penaeus vannamei, comprising the following steps:

[0040] 1) Add water to the gelatin, heat at 40-50° C. to fully dissolve, and obtain an 8-12 (w / v)% gelatin aqueous solution.

[0041] 2) Add glycerin with 2-4 (w / v)% gelatin volume to the aqueous gelatin solution, and stir well and evenly.

[0042] 3) Then add ε-polylysine solution and astaxanthin extracted from the shell of Penaeus vannamei, so that the concentrations of ε-polylysine and astaxanthin in the resulting solution are not less than 0.13g / L and 15 μg / mL.

[0043] 4) Homogenize the solution obtained in step 3) at 10000-15000 r / min for 5-15 seconds, and adjust the pH value to 3.5-4.5.

[0044]5) Add chitosan powder (deacetylation degree is 80.0-95.0%) of gelatin mass 4-8% to the solution obtained in step 4), and stir under dark conditions until chitosan is completely dissolved to obtai...

Embodiment 1

[0048] 1. Antibacterial effect of ε-polylysine on spoilage bacteria (mixed bacteria solution) of Penaeus vannamei Using the spoilage bacteria (mixed bacteria) of Penaeus vannamei as indicator bacteria, different concentrations of ε-polylysine (final concentration 2.0, 1.0, 0.5, 0.25, 0.13g / L) on the antibacterial effect of vannamei spoilage bacteria, the results are shown in figure 1 . figure 1 The results showed that the bacteriostatic rate of ε-polylysine on spoilage bacteria of Penaeus vannamei did not increase significantly with the increase of the concentration of ε-polylysine (p>0.05), indicating that the low concentration of ε-polylysine The acid still has a very high antibacterial effect on the spoilage bacteria of Penaeus vannamei.

[0049] 2. In vitro antioxidant activity of astaxanthin derived from Penaeus vannamei

[0050] Astaxanthin was extracted from the shrimp shell of Penaeus vannamei by absolute ethanol solvent extraction method, and used for the preparatio...

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Abstract

The invention relates to the field of food packaging materials, and discloses a preparation method of an astaxanthin-containing biological antibacterial composite membrane for inhibiting spoilage bacteria of penaeus vannamei boone. According to the preparation method, gelatin and chitosan are used as composite membrane matrixes, and epsilon-polylysine with an antibacterial effect and high-oxidation-resistance astaxanthin extracted from penaeus vannamei boone shells are added on the basis, so that the biological antibacterial composite membrane is prepared, wherein the antibacterial membrane can effectively inhibit the proliferation of spoilage bacteria of penaeus vannamei boone. According to the invention, it is accidentally found that addition of astaxanthin can bring oxidation resistanceto the composite membrane and can significantly improve the physical properties of the chitosan-gelatin-based membrane at a specific content. The preparation method of the composite membrane is simple, convenient and easy to operate and can be used for expanded production.

Description

technical field [0001] The invention relates to the field of food packaging materials, in particular to a preparation method of an astaxanthin-containing biological antibacterial composite film for inhibiting spoilage bacteria of Penaeus vannamei. Background technique [0002] Food spoilage is usually caused by microbial contamination, especially for foods with high water content and high protein content, if the preservation measures are not ideal, spoilage will easily occur. In order to eliminate spoilage bacteria and prolong the shelf life of food, people have developed a variety of fresh-keeping methods during food storage. Among them, the incorporation of active agents into film polymer matrix as a packaging material is considered to be an effective method for food preservation. [0003] As a natural protein, gelatin is partially hydrolyzed from collagen. It is edible and biodegradable, and is widely used in polymer films. Chitosan is a natural polysaccharide obtained ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L89/00C08L67/04C08L5/08C08K5/132C08K5/053A23B4/18A23B4/20
CPCC08J5/18A23B4/18A23B4/20C08J2389/00C08J2467/04C08J2405/08C08K5/132C08K5/053A23V2002/00A23V2200/10A23V2250/2042
Inventor 宋茹韦荣编
Owner ZHEJIANG OCEAN UNIV
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