Check patentability & draft patents in minutes with Patsnap Eureka AI!

Preparation method of biological sample with cilia suitable for scanning electron microscope

A technology of biological samples and scanning electron microscopy, which is applied in the field of preparation of ciliated biological samples, can solve the problems of not being able to show the real shape, the collapse and reunion of cilia, and the difficulty of shooting, etc. It achieves good results, reduces the viscosity of mucus, and the method is simple and easy. Effect

Inactive Publication Date: 2020-05-19
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The reason why it is difficult to photograph biological samples with cilia is mainly because the surface of the sample is prone to secrete mucus, which adheres to the cilia, causing the cilia of the treated sample to collapse and reunite, and the true appearance cannot be displayed under the electron microscope.
The usual treatment is to wash with PBS, but the effect is not ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of biological sample with cilia suitable for scanning electron microscope
  • Preparation method of biological sample with cilia suitable for scanning electron microscope
  • Preparation method of biological sample with cilia suitable for scanning electron microscope

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation method steps of scanning electron microscope scallop larvae are as follows:

[0026] (1) Get fresh scallop larvae, wash the larvae three times with 0.1M phosphate buffer solution, and soak for 5 minutes with 3% hyaluronidase solution;

[0027] (2) Wash the larvae 3 times with 0.1M phosphate buffer solution, 3 min each time;

[0028] (2) Soak the larvae in 2.5% glutaraldehyde fixative solution and place them in a refrigerator at 4°C for 12 hours;

[0029] (5) Wash the larvae 5 times with 0.1M phosphate buffer solution, 5 min each time;

[0030] (6) Dehydration with 30%, 50%, 70%, 80%, 90%, 95%, 100% gradient ethanol in sequence, each time for 8 minutes;

[0031] (7) Replace with 100% acetone twice, each time for 8 minutes;

[0032] (8) Dry the sample with a critical point dryer;

[0033] (9) After the drying is completed, take out the sample and stick it on the sample stage with conductive tape;

[0034] (10) Coating the sample with an ion sputtering...

Embodiment 2

[0038] The sample used in this embodiment is a mammal: rat nasal cavity epithelial tissue.

[0039] The preparation method steps of scanning electron microscope rat nasal cavity epithelial tissue are as follows:

[0040] (1) Get fresh rat nasal epithelial tissue, and wash the sample three times with 0.1M phosphate buffer solution;

[0041] (2) Soak with 3% hyaluronidase solution for 5 minutes;

[0042] (3) Wash the sample 3 times with 0.1M phosphate buffer solution, 3 minutes each time;

[0043] (4) Dip the sample in 2.5% glutaraldehyde fixative solution and place it in a refrigerator at 4°C for 12 hours;

[0044] (5) Wash the sample 5 times with 0.1M phosphate buffer solution, 5 min each time;

[0045] (6) Dehydration with 30%, 50%, 70%, 80%, 90%, 95%, 100% gradient ethanol in sequence, each time for 8 minutes;

[0046] (7) Replace with 100% acetone twice, each time for 8 minutes;

[0047] (8) Dry the sample with a critical point dryer;

[0048] (9) After the drying is ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biology, and particularly relates to a preparation method of a biological sample with cilia suitable for a scanning electron microscope. The preparationmethod comprises the steps of sample cleaning, tissue fixation, dehydration, replacement, drying, metal spraying and the like. Before a to-be-treated sample is fixed, a hyaluronidase solution is usedfor pretreatment. The method for preparing the scanning electron microscope biological sample with cilia provided by the invention is simple, feasible and good in effect; by adopting the method, themorphological structure of cilia on the surface of the biological sample can be better maintained, the cilia is not easy to deform, shrink and agglomerate, a clear cilia structure scanning electron microscope image can be obtained, and a new method is provided for the scanning electron microscope sample preparation of the cilia biological sample.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing a ciliated biological sample suitable for a scanning electron microscope. Background technique [0002] The purpose of the scanning electron microscope is to observe the surface morphology of the sample, and to fully and truly display the observed surface of the sample is the first condition for finally obtaining a clear image of the morphology. Therefore, the pretreatment of samples is the key process of electron microscope observation, and the quality of its treatment effect is directly related to the experimental results of electron microscope observation. [0003] The pretreatment of biological samples for scanning electron microscopy generally includes steps such as sample cleaning, tissue fixation, dehydration, replacement, drying, and gold spraying. However, the surface of biological samples with cilia is easy to secrete mucus to adhere to t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N23/2202
CPCG01N23/2202G01N2223/07
Inventor 孙园园刘伟
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com