A kind of peony 2s albumin and its extraction method and application
An extraction method and albumin technology, applied in the protein field, can solve the problems of low level of comprehensive development and utilization, and achieve the effect of simple process
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Embodiment 1
[0041] 1, degreasing treatment
[0042] After pulverizing the peony seed meal was pulverized with a high-speed pulverizer, it was added to the gas ratio of 1 g: 5 mL to the n-hexane, stirred for 30 min, stir filtered, repeated twice, mixed withdrawal, and evaporated to recover n-hexane. Peony seeds were dried at 25 ° C.
[0043] 2, PBS buffer soaking
[0044] Take 20 g of peony seeds to degrease powder, adding 160 ml of 0.015 mol / l pH of 7.0, PBS buffer, extract 12 h at 4 ° C, after extracting the high speed homogenating machine, then centrifuged at 4000 r / min for 30 min Take the supernatant to give the protein extract, with the protein extraction rate of 38.57%.
[0045] 3, ammonium ammonium sulfate sedimentation crude protein
[0046] The ammonium sulfate is added to the protein extract from the 2nd protein extract to 20%, 4 ° C, and then centrifuged at 12000 r / min for 20 min to remove the supernatant; continued to add ammonium sulfate to a saturation of 40% in the superna...
Embodiment 2
[0077] In this embodiment, the peony seed meal in Example 1 was replaced with Peony Seed, and the other steps were identical to Example 1 to obtain a target protein peony 2S albumin (PS-2S-Alb) 0.98 g, purity 95% or more.
Embodiment 3 Embodiment 1
[0078] Example 3 Example 1 Antioxidant activity of peony 2S albumin extracted
[0079] This embodiment uses the clearance of DPPH capabilities, removes hydroxyl, clearing the four methods of ABTS free radicals and FRAP reduction capability, performing body external anti-oxidative activity evaluation of purified peony 2S albumin, and simultaneously with positive control of ascorbic acid activity Compare.
[0080] (1) Determination of DPPH free radical clearance capability
[0081] Take 3.0 ml of a series of concentrations of protein aqueous solution, 3.0 ml of 100 μmol / L of DPPH-ethanol solution, and prevent light at room temperature for 30 min, and the absorbance value of the reaction mixture was measured at 517 nm; replace the protein sample with distilled water, the same conditions The absorbance value A0 is measured; the absorbance value B determined under the same conditions with ethanol solution. And as a positive control with ascorbic acid (VC), each sample was parallel. T...
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