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Gene related to dormancy release of flower bulbs, and application thereof

A flower and bulb technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., achieves important theoretical significance and application potential, reduces production costs, and shortens the growth cycle

Active Publication Date: 2020-05-22
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Little is known about how tulip flower buds and seeds respond to low temperature and the molecular mechanisms that mediate differences in low temperature requirements among cultivars

Method used

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  • Gene related to dormancy release of flower bulbs, and application thereof
  • Gene related to dormancy release of flower bulbs, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Tulip dormancy release-related gene DRR1( D ormancy R release R elated 1) Obtained:

[0021] 1. Cloning of DRR1 gene

[0022] In this study, the transcriptome analysis of two tulip varieties with significant differences in low temperature requirements was carried out to obtain the differentially expressed gene DRR1. Then, according to the full-length transcriptome sequencing results of the third generation of tulip, primers were designed to clone the DRR1 sequence using cDNA as a template. Sequencing results showed that the CDS region of the DRR1 gene was highly homologous (96.21% homology) to the sequence of the tulip GBOF-1 gene (GenBank No. AAD56411.1). Then, using the tulip genome DNA as a template, the genome sequence of DRR1 was cloned, and the sequence analysis showed that DRR1 contained 5 exons and 4 introns. The structural diagram of the gene is shown in figure 1 . The results of protein sequence analysis showed that DRR1 encodes a bHLH transcription facto...

Embodiment 2

[0026] Application of tulip DRR1 gene in the field of plant dormancy relief:

[0027] The CDS sequence (SEQ ID NO.1) of the DRR1 gene was constructed into the pCAMBIA1300 vector, and transformed into Agrobacterium GV3101, and the genetic transformation of Arabidopsis was carried out by using the flower soaking method to obtain multiple transgenic plants overexpressing DRR1, and then detected by the seed germination experiment Regulation of tulip DRR1 gene in Arabidopsis seed dormancy. Experimental results such as figure 2 As shown, OX-1 and OX-3 in the figure represent two independent DRR1 overexpression Arabidopsis transgenic lines, which are used to illustrate the reproducibility of the results, and WT represents the common Arabidopsis control without transgenic treatment. Depend on figure 2 It can be seen that the germination rate of OX-1 and OX-3 transgenic plants is very good under low-temperature vernalization treatment or without low-temperature vernalization treatm...

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Abstract

The invention belongs to the technical field of plant genetic engineering, and in particular relates to a gene related to dormancy release of flower bulbs, and an application thereof. The gene is a DRR1 gene, and a nucleotide sequence of the DRR1 gene is shown in SEQ ID NO:1. The invention also relates to the cloning of a promoter of the DRR1 gene. The phenotype analysis of agrobacterium-mediatedsteadily transformed Arabidopsis plants is performed, and the result shows that the gene has the significant function of replacing low temperature to break plant dormancy, regulating seed germinationand flower bud growth. The discovery of the DRR1 gene provides a new genetic resource for cultivating new liliaceous flower bulb varieties that do not need low-temperature treatment or need less low-temperature requirements. At the same time, a new genetic resource for ornamental gardening is provided. The utilization of the gene greatly shortens a growth cycle of flower bulbs, and reduces the economic cost of seed ball post-harvest treatment and forcing culture.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a gene related to dormancy relief of bulbous flowers and its application. Background technique [0002] Tulips and other bulbous flowers are important autumn bulbous flowers and play an important role in the international flower trade. Worldwide, the production and consumption of tulips have been developing at a high speed. In 2017, my country imported about 350 million tulip bulbs, while domestically produced tulip bulbs were less than 5 million. At the same time, due to the unqualified post-harvest treatment technology, domestic tulip bulbs have blind flowers or inconsistent flowering periods, and the viewing effect is poor, which further limits the application space of domestic tulip bulbs. In the stage of postharvest treatment of bulbs, temperature is a key factor affecting flower bud differentiation of tulip bulbs. After the tulip bulbs are harvested, du...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/113C12N15/82A01H5/10A01H6/20A01H6/56
CPCC07K14/415C12N15/8267
Inventor 王艳平产祝龙赵慧敏
Owner HUAZHONG AGRI UNIV
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