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Preparation method of composite electrospun tissue engineering scaffold material for anti-tumor, engineering scaffold material and application thereof

A tissue engineering scaffold and anti-tumor technology, applied in textiles and papermaking, artificial silk manufacturing, conjugated synthetic polymer artificial filaments, etc., can solve the problems of degradation product cells, tissue damage, fast degradation speed, poor mechanical properties, etc. , to achieve good mechanical properties and biocompatibility, promote tissue repair and regeneration, and stabilize the skeleton structure support

Active Publication Date: 2021-01-08
THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing fiber scaffold preparation materials are absorbable synthetic polymer materials such as PLA and PCL, which have poor mechanical properties, the structure is easily damaged by external forces, and the degradation speed is too fast, and the degradation products have certain damage to cells and tissues. , which limit their application in cell scaffolds, tissue repair
In addition, existing fibrous scaffolds still have problems of adhesion and related complications in the body

Method used

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  • Preparation method of composite electrospun tissue engineering scaffold material for anti-tumor, engineering scaffold material and application thereof
  • Preparation method of composite electrospun tissue engineering scaffold material for anti-tumor, engineering scaffold material and application thereof
  • Preparation method of composite electrospun tissue engineering scaffold material for anti-tumor, engineering scaffold material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of a composite electrospun tissue engineering scaffold material for anti-tumor, comprising the steps of:

[0029] S1. Preparation of poly(3-hydroxybutyrate-4-hydroxybutyrate) / L-polylactic acid electrospun fibers: Weigh 40 mg of medical-grade P34HB powder (purity greater than 99.5%) and 40 mg of medical-grade L-lactic acid The powder was dissolved in a composite solvent of 1000 mL of dichloromethane and dimethylformamide (DMF) (the volume ratio of dichloromethane and dimethylformamide was 3:7), stirred and dissolved by a magnetic stirrer for 2 hours, and the temperature was 60 ℃, stir evenly, and make P34HB / PLLA solution.

[0030] S2. Preparation of polyhydroxyalkanoate / L-polylactic acid composite electrospun fiber mixed solution loaded with epirubicin: Weigh 5 mg of epirubicin and add poly(3-hydroxybutyrate-4- Hydroxybutyrate) / L-polylactic acid electrospun fiber solution, continue magnetic stirring until the epirubicin is completely dissolved.

[...

Embodiment 2

[0033] A preparation method of a composite electrospun tissue engineering scaffold material for anti-tumor, comprising the steps of:

[0034] S1. Preparation of poly(3-hydroxybutyrate-4-hydroxybutyrate) / poly-L-lactic acid electrospun fibers: Weigh 40 mg of medical-grade P34HB powder (purity greater than 99.5%) and 32 mg of medical-grade poly-L-lactic acid The powder was dissolved in a composite solvent of 1000 mL of dichloromethane and dimethylformamide (DMF) (the volume ratio of dichloromethane and dimethylformamide was 6:4), stirred and dissolved by a magnetic stirrer for 2 hours, and the temperature was 60 ℃, stir evenly, and make P34HB / PLLA solution.

[0035] S2. Preparation of polyhydroxyalkanoate / L-polylactic acid composite electrospun fiber mixed solution loaded with epirubicin: Weigh 4 mg of epirubicin and add poly(3-hydroxybutyrate-4- Hydroxybutyrate) / L-polylactic acid electrospun fiber solution, continue magnetic stirring until the epirubicin is completely dissolved...

Embodiment 3

[0038] A preparation method of a composite electrospun tissue engineering scaffold material for anti-tumor, comprising the steps of:

[0039] S1. Preparation of poly(3-hydroxybutyrate-4-hydroxybutyrate) / L-lactic acid electrospun fibers: Weigh 40 mg of medical-grade P34HB powder (purity greater than 99.5%) and 48 mg of medical-grade L-lactic acid The powder was dissolved in a composite solvent of 1000 mL of dichloromethane and dimethylformamide (DMF) (the volume ratio of dichloromethane and dimethylformamide was 2:8), stirred and dissolved by a magnetic stirrer for 2 hours, and the temperature was 60 ℃, stir evenly, and make P34HB / PLLA solution.

[0040] S2. Preparation of polyhydroxyalkanoate / L-polylactic acid composite electrospun fiber mixed solution loaded with epirubicin: Weigh 6 mg of epirubicin and add poly(3-hydroxybutyrate-4- Hydroxybutyrate) / L-polylactic acid electrospun fiber solution, continue magnetic stirring until the epirubicin is completely dissolved.

[0041...

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Abstract

The invention discloses a preparation method of a composite electro-spun tissue engineering scaffold material, the engineering scaffold material and application thereof. The preparation method of thecomposite electro-spun tissue engineering scaffold material comprises the steps of adopting a poly (3-hydroxybutyrate-4-hydroxybutyrate) copolymer and levorotatory polylactic acid mixed material to prepare electro-spun fibers through an electrostatic spinning technology, and spinning epirubicin into a P34HB / PLLA fiber scaffold through mixed electro-spinning to prepare the epirubicin-loaded P34HB / PLLA composite electro-spun fibers. The epirubicin-loaded P34HB / PLLA composite electro-spun fibers of the composite electro-spun tissue engineering scaffold material prepared by the preparation methodhave good mechanical property and biocompatibility, proper degradation speed and excellent anti-tumor property, and have wide application prospect in the biomedical field of tumor treatment.

Description

technical field [0001] The invention relates to the field of medical biomaterials, more specifically, the invention relates to a preparation method of a composite electrospun tissue engineering scaffold material for anti-tumor, an engineering scaffold material and applications thereof. Background technique [0002] Polyhydroxyalkanoate (PHA) is a linear saturated polyester whose fiber waste can be completely absorbed by the natural environment and has good biodegradability and biocompatibility, while poly 3-hydroxybutyrate / 4- Hydroxybutyrate (P34HB) is the latest generation of biodegradable materials in the PHA family. Fibrous scaffolds prepared based on electrospinning technology have a similar fiber structure to natural extracellular matrix (ECM), and thus are widely used in cell proliferation culture, drug delivery, biosensors and other fields. With the deepening of tissue engineering research, electrospinning technology continues to develop, and different spinning proce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D01F8/14D01F1/10A61L27/18A61L27/54A61L27/58
CPCA61L27/18A61L27/54A61L27/58A61L2300/416D01F1/10D01F8/14C08L67/04
Inventor 叶雷郭强王辉王德娟吕金艳司徒卫余柳松黄榕康过文泰王怀明秦秀森卫应齐焦燕
Owner THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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