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Preparation method of silk fibroin/gelatin fiber reinforced chitosan composite haemostatic material

A silk fibroin, fiber-reinforced technology, applied in the direction of cellulose/protein conjugated artificial filament, animal fiber, fiber processing, etc., can solve the problems of insufficient mechanical properties of hemostatic materials, etc.

Active Publication Date: 2020-05-22
HUAIYIN INSTITUTE OF TECHNOLOGY
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Problems solved by technology

[0004] Purpose of the invention: Aiming at the problem of insufficient mechanical properties of hemostatic materials in the prior art, the present invention proposes a method for preparing a silk fibroin / gelatin fiber reinforced chitosan composite hemostatic material, in which silk fibroin and gelatin are prepared into composite fibers , and use the composite fiber as a reinforcing material to add it to the chitosan matrix, and prepare a ternary composite hemostatic material by means of freeze-drying method. The hemostatic material improves the mechanical properties of the pure chitosan material and has excellent hemostatic performance

Method used

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  • Preparation method of silk fibroin/gelatin fiber reinforced chitosan composite haemostatic material

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[0022] The invention relates to the fields of tissue engineering and biomedical materials, and discloses a preparation method of a silk fibroin / gelatin fiber reinforced chitosan composite hemostatic material, comprising the following steps:

[0023] S1: Blend 10-20% silk fibroin aqueous solution with 4% gelatin aqueous solution, so that the mass ratio of the two is controlled at 1:1-1:4. After mixing evenly, concentrate the mixed solution to the silk fibroin mass fraction 30%~40%;

[0024] S2: Prepare the mixed solution described in S1 into a silk fibroin / gelatin composite fiber mat by electrospinning. The conditions of the electrospinning method are: the spinning voltage is 14-20 kV, and the receiving distance from the needle to the receiver is 10~15 cm, the extrusion speed of the micro syringe pump is 0.6~1.2 mL / h, the ambient temperature is 10~20 ℃, and the humidity is 40~60 RH%;

[0025] S3: Use 80 vol.% ethanol aqueous solution to post-treat the silk fibroin / gelatin comp...

Embodiment 1

[0030] Degumming silkworm cocoons with boiled sodium carbonate aqueous solution, dissolving degummed silk in lithium bromide (LiBr) aqueous solution, centrifuging, filtering, and dialysis to remove impurities and LiBr to obtain regenerated silk fibroin (RSF) aqueous solution, concentrated to RSF quality The percentage is 10% for use; gelatin (GEL) is dissolved in water to prepare a 4% GEL aqueous solution; 10% RSF aqueous solution and 4% GEL aqueous solution are blended (control GEL and RSF mass ratio is 1: 1), the mixed solution was concentrated to 30% RSF mass fraction, and the RSF / GEL composite fiber mat was obtained by electrospinning. Among them, the experimental parameters of the electrospinning method are: the relative humidity is 40±5%, the temperature is 22±5°C, the inner diameter of the needle is 0.6 mm, the voltage is 20 kV, and the receiving distance from the needle to the receiver (aluminum foil) is 10 cm , the extrusion rate of the micro-injection pump was 1.2 mL...

Embodiment 2

[0033] The difference between embodiment 2 and embodiment 1 is: embodiment 2 controls the mass ratio of GEL and RSF to be 1:4, and other operations are the same as embodiment 1, as follows:

[0034] Degumming silkworm cocoons with boiled sodium carbonate aqueous solution, dissolving degummed silk in lithium bromide (LiBr) aqueous solution, centrifuging, filtering, and dialysis to remove impurities and LiBr to obtain regenerated silk fibroin (RSF) aqueous solution, concentrated to RSF quality The percentage is 10% for use; gelatin (GEL) is dissolved in water to prepare a 4% GEL aqueous solution; 10% RSF aqueous solution is blended with 4% GEL aqueous solution (the mass ratio of GEL to RSF is 1:4 ), the mixed solution was concentrated until the mass fraction of RSF was 30%, and the RSF / GEL composite fiber mat was obtained by electrospinning. Wherein, the experimental parameters of the electrospinning method are the same as those in Example 1. The RSF / GEL composite fiber felt wa...

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Abstract

The present invention relates to a preparation method of a silk fibroin / gelatin fiber reinforced chitosan composite haemostatic material. The preparation method comprises the following steps that S1,a silk fibroin aqueous solution is mixed with a gelatin aqueous solution, and a mixed solution is concentrated until a certain concentration of silk fibroin is reached; S2, through an electrostatic spinning method, the mixed solution in the step S1 is prepared into a silk fibroin / gelatin composite fiber felt; S3, the silk fibroin / gelatin composite fiber felt is subjected to post-treatment and high-speed shearing, thereby preparing fiber water dispersion liquid; S4, a chitosan solution is prepared; S5, the composite fiber aqueous solution and the chitosan solution are mixed according to a certain mass ratio; and S6, a mixed solution in the step S5 is freeze-dried to obtain a three-dimensional porous scaffold, and post-treatment is performed to obtain a silk fibroin / gelatin / chitosan scaffold. Compared with the prior art, the silk fibroin and gelatin are prepared into composite fiber, and the composite fiber as a reinforcing material is added to a chitosan matrix to prepare a ternary composite hemostatic material, thereby improving the mechanical properties and the hemostatic performance of a pure chitosan material.

Description

technical field [0001] The invention relates to the fields of tissue engineering and biomedical materials, in particular to a preparation method of a silk fibroin / gelatin fiber reinforced chitosan composite hemostatic material. Background technique [0002] Traumatic bleeding is a problem often encountered in daily accidents. If it is not treated in time or handled improperly, it may be life-threatening. Therefore, it is very important to develop hemostatic materials with excellent comprehensive properties. There are many materials currently used in biomedical anticoagulant research, such as chitosan, gelatin, sodium alginate and fibrin. Chitosan is the only positively charged alkaline polysaccharide in nature. It not only has excellent hemostatic effect, but also has good biodegradability and antibacterial properties. The prepared hemostatic material has been widely used in clinic. However, the hemostatic material prepared from a single chitosan has limited hemostatic effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M15/03D01F8/02A61L24/00A61L24/04D01D5/00D04H1/4382D04H1/76D06M101/10
CPCD06M15/03D01F8/02A61L24/043A61L24/0015A61L24/0036A61L24/001D04H1/76D04H1/4382D01D5/003D01D5/0092D01D5/0061A61L2400/04A61L2300/236A61L2300/252D06M2101/10C08L89/00C08L5/08
Inventor 张超王心如叶玮张秋阳丁红燕
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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