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Application of sturgeon protein peptide to preparation of immunomodulatory preparation

An immune regulation and protein peptide technology, applied in the fields of application, metallothionein, and hydrolyzed protein components, etc., can solve problems such as obvious side effects, inaccurate immune regulation effects, and common sequelae

Pending Publication Date: 2020-05-29
HUBEI YANGGUANG SCI & TECHCAL MEDICINES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the dosage of hormone immunomodulatory preparations is difficult to control, and sequelae are common; alkylating agents and antimetabolites have obvious cytotoxicity, so they should be used with caution; other immunomodulatory drugs also have obvious side effects or inaccurate immune regulatory effects.

Method used

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  • Application of sturgeon protein peptide to preparation of immunomodulatory preparation
  • Application of sturgeon protein peptide to preparation of immunomodulatory preparation
  • Application of sturgeon protein peptide to preparation of immunomodulatory preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Effect experiment on the survival rate of monocyte-macrophage RAW264.7 cells

[0042] Mouse mononuclear macrophage RAW264.7 cultured in vitro was used as a cell model to evaluate the immunomodulatory activity of sturgeon protein peptides.

[0043] Macrophages are the main components of the mononuclear macrophage system. They have multiple functions of phagocytosis of foreign invading pathogenic microorganisms, processing and presentation of antigens. They kill foreign pathogens by phagocytosis and secretion of cytokines, which play a key role in maintaining human health. effect. RAW264.7 is a mononuclear macrophage, which includes macrophages in tissues, premonocytes in bone marrow, and monocytes in peripheral blood. important role in defense. It can not only initiate the innate immune (non-specific immune) response, but also participate in the cellular immune (specific immune) response, and play an important role in the body's resistance to microbial infect...

Embodiment 2

[0051] Example 2 Effect experiment of cellular NO secretion

[0052] Take the RAW264.7 cells in the logarithmic growth phase, adjust the cell concentration to 1×10^6 cells / ml after counting the cells, inoculate them in a 24-well plate, culture them in 5% CO2 at 37°C for 24 hours, discard the supernatant, Add 100ul DMEM basal medium (without serum) to the blank control group; give different concentrations (12.5-500ug / ml) of sturgeon protein peptides (prepared in basal medium) to the experimental group, continue to culture for 24 hours, and collect the supernatant at 2500r / min Centrifuge for 10min, take 100ul respectively in the microtiter plate, then add the same amount of Griess reagent, let it stand at room temperature for 30min, then measure the absorbance at 540nm. The nitrite (NO2-) content in the supernatant was calculated according to the standard curve.

[0053] NO is also an important cytokine in the immune response, which can be secreted by activated macrophages. It ...

Embodiment 3

[0056] Embodiment 3 human experiment

[0057] Subject patient Liu, female, 62 years old, was diagnosed with 2019-nCoV coronavirus infection on January 23, 2020, and was accompanied by underlying hypertension. image 3 As shown in the left picture, the result is a ground-glass shadow in both lungs. From January 24, 2020 to January 29, 2020, in addition to supportive therapy, use the sturgeon protein peptide provided by the invention, three times a day, 30 grams at a time, and take it with warm water. On January 29, 2020, the radiographic examination was performed again, and the results of the posteroanterior chest radiograph were as follows: image 3 As shown in the picture on the right, the lung lesions were significantly improved.

[0058] It is shown that the sturgeon protein peptide provided by the present invention has an adjuvant therapeutic effect against viral infection, especially an adjuvant therapeutic effect against coronavirus infection. Since the coronavirus ca...

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Abstract

The invention provides application of a sturgeon protein peptide to preparation of an immunomodulatory preparation. The sturgeon protein peptide contains 1.2 to 1.5 mg / g of metallothionein peptides, and the average molecular weight is below 250 Da. Experiments prove that the immune cell proliferation can be stimulated, and the immune response can be started, so that an immunomodulatory effect is achieved; and the sturgeon protein peptide can be used for preparing an immunoenhancing preparation and can be used for auxiliary treatment of virus infection and malignant tumors to achieve the effectof bidirectionally regulating the immunity. Meanwhile, the food-grade safety is realized, and the sturgeon protein peptide is also suitable for being used as food and health-care products with the immunomodulatory effect for daily health care.

Description

technical field [0001] The invention belongs to the field of biological products, and more specifically relates to the application of sturgeon protein peptides in the preparation of immunomodulatory preparations. Background technique [0002] Immunomodulatory preparations refer to a class of substances that can regulate, enhance, excite and restore the immune function of the body. Immunomodulatory agents include hormones, such as dexamethasone, prednisone, alkylating agents, such as cyclophosphamide, antimetabolites, such as methotrexate, and some natural agents, such as antithymocyte globulin, antihuman lymphoid Cellular immunoglobulin, certain fungal polysaccharides, such as lentinan. [0003] However, the dosage of hormone immunomodulatory preparations is difficult to control, and sequelae are common; alkylating agents and antimetabolites have obvious cytotoxicity, so they should be used with caution; other immunomodulatory drugs also have obvious side effects or inaccur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01C12P21/06A61P37/04A61P37/02A61P31/12A61P35/00A23L33/18
CPCA61K38/012C12P21/06C07K14/825A61P37/04A61P37/02A61P31/12A61P35/00A23L33/18A23V2002/00A23V2200/324A23V2200/308
Inventor 林国华林靖杰黄文樊锋凯
Owner HUBEI YANGGUANG SCI & TECHCAL MEDICINES
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