Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

MicroRNA serum marker, primer set, kit and application for identifying sex of sturgeon

A serum marker and kit technology, applied in DNA/RNA fragmentation, recombinant DNA technology, microbial determination/inspection, etc., can solve the problems of long sexual maturity, waste of resources of male individuals, and inability to distinguish male and female by morphology, Achieve high quantitative sensitivity and specificity, simple method operation, and good reproducibility.

Active Publication Date: 2020-07-24
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no obvious secondary sexual characteristics between male and female sturgeon in adolescence or even adulthood, and it is impossible to distinguish between male and female morphologically, and the sexual maturity takes a long time
It is not possible to carry out intensive breeding according to the male and female sexes or to raise them in a certain sex ratio, resulting in a waste of resources for raising too many male individuals, increasing the cost of breeding, and hindering the rapid development of the industrialization of sturgeon farming

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MicroRNA serum marker, primer set, kit and application for identifying sex of sturgeon
  • MicroRNA serum marker, primer set, kit and application for identifying sex of sturgeon
  • MicroRNA serum marker, primer set, kit and application for identifying sex of sturgeon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Screening of differentially expressed miRNAs in gonads of Acipenser schneideri

[0041] Differentially expressed miRNAs and gonad-specific conserved miRNAs (dre-miR-34b and dre-miR-34c) in the gonads of Acipenser schrenckii with |Log2(FC)|>2 (FC, fold change), removed the family similarity 100% miRNA, 36 miRNAs were retained. The list of miRNA gene information is shown in Table 1 below:

[0042] Table 1. Sequence information of 36 miRNA genes

[0043]

[0044] 2. Collect serum

[0045] A batch of 3-year-old Sturgeon's sturgeon was taken, and the body weight, body length and total length were measured. Paraffin sections of the gonad tissue were made and hematoxylin and eosin staining was used for histological observation, and the sex was accurately determined. Blood was collected from the tail vein and placed in a 1.5 mL sterile EP tube, let stand at room temperature for 30 minutes, centrifuge at 3500 rpm for 15 minutes in a low-temperature centrifuge, separate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides microRNA serum markers, primer sets, kits and applications for identifying the sex of sturgeons. The present invention utilizes high-throughput small RNA sequencing technology and microRNA chip technology to screen out the miRNA types and expression levels differentially expressed in the gonads (testis and ovary) of 3-year-old Stryker's sturgeon, and screen 36 of the miRNAs to screen the serum of Stryker's sturgeon sex miRNA markers, through conventional serum RNA extraction, cDNA acquisition and real-time quantitative PCR and other technologies, a new method of serum miRNA markers for sex identification of sturgeon was established, and it was tested in young Sturgeon's sturgeon and Western hybrid sturgeon. It has been verified that the method of the present invention is simple to operate and has good repeatability. It is a new breakthrough in the field of efficient and early identification of sturgeon sex, and is suitable for popularization and application. question.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to microRNA serum markers, primer sets, kits and applications for identifying the sex of sturgeons. Background technique [0002] Sturgeon is an extremely rare fish. Caviar made from sturgeon roe is rich in essential amino acids, multiple unsaturated fatty acids, vitamins and other substances. It has high nutritional value and enjoys the reputation of "black gold". Its international price is as high as 800-900 US dollars per kilogram in 2010, so female sturgeon has higher economic value than male sturgeon. At present, the production of sturgeon farming and caviar, as emerging industries, is developing extremely rapidly in my country. According to preliminary statistics, the annual output of commercial sturgeon farming in my country has exceeded 20,000 tons, accounting for 66.17% of the world's total output of 72.46% of the output, it seems to be the largest sturgeon farming ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6879C12N15/11
CPCC12Q1/6888C12Q1/6879C12Q2600/178C12Q2600/124
Inventor 张秀娟陈金平吴文化周佳滨李林妙江海英
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com