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Application of cotton GhACO gene to plant flowering promotion

A gene and plant technology, applied in the application field of GhACO gene in promoting plant flowering, can solve the problem of lack of gene resources and achieve the effect of reducing the number of rosette leaves

Inactive Publication Date: 2020-06-02
INST OF COTTON RES CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are still few studies on genes related to cotton precociousness, and there is a lack of effective genetic resources for genetic engineering breeding of cotton precocious varieties

Method used

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  • Application of cotton GhACO gene to plant flowering promotion
  • Application of cotton GhACO gene to plant flowering promotion
  • Application of cotton GhACO gene to plant flowering promotion

Examples

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Embodiment

[0024] The following examples are used here to demonstrate preferred embodiments of the invention. Those skilled in the art will understand that the technology disclosed in the following examples represents the technology discovered by the inventor that can be used to implement the present invention, and therefore can be regarded as a preferred solution for implementing the present invention. However, those skilled in the art should understand from this specification that many modifications can be made to the specific embodiments disclosed herein, and the same or similar results can still be obtained without departing from the spirit or scope of the present invention.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which the present invention belongs. The public quotations herein and the materials cited by them will be incorporated by reference. .

[0026] Those skil...

Embodiment 2

[0122] Example 2 Construction of PBI121-GhACO plant expression vector

[0123] 1 Obtain the target gene fragment with specific restriction site

[0124] The cDNA sequence of the cloned GhACO gene was designed to contain suitable enzyme cleavage site primers at the start codon ATG and the stop codon respectively. The restriction sites used are Xba I (T / CTAGA) and Sma I (CCC / GGG).

[0125] The primer sequence of the restriction site of GhACO gene is as follows:

[0126] Upstream primer F (SEQ ID No. 5):

[0127] 5'-CTAGTCTAGAATGGCAGAAATAAGCTTAGAACG-3'

[0128] Downstream primer R (SEQ ID No. 6):

[0129] 5'-TCCCCCGGGTTAAGGTGGGCTTGCCTGCATCA-3'

[0130] The amplified target fragments with restriction sites were ligated to the pGEM-T Easy cloning vector, transformed into DH5α competent cells, and the intermediary recombinants with no mutations in the sequence were screened out by PCR and restriction enzyme digestion verification and sequence determination.

[0131] 2 Construction of pBI121-GhAC...

Embodiment 3

[0138] Example 3 Transformation of recombinant vector pBI121-GhACO into Arabidopsis thaliana using Agrobacterium-mediated method

[0139] 1 Preparation of LBA4404 Agrobacterium Competent

[0140] Use CaCl 2 The specific process of preparing competent cells is as follows:

[0141] 1) Pick a single colony and inoculate it in 4mL LB liquid medium containing antibiotics, and cultivate overnight at 28°C and 190rpm;

[0142] 2) Transfer to 80 mL of antibiotic-containing LB liquid medium at a ratio of 1:90, culture to OD at 28°C and 170 rpm 600 = 0.6;

[0143] 3) After ice bathing for 30 minutes, transfer the bacterial solution to a 50mL centrifuge tube, centrifuge at 4°C, 5,000 rpm for 10 minutes, and discard the supernatant;

[0144] 4) Add 5mL pre-chilled 70mM CaCl 2 , Gently suspend, let stand on ice for 20 min, 4℃, 5,000 rpm, centrifuge for 5 min, discard the supernatant;

[0145] 5) Add 2mL pre-chilled 70mM CaCl containing 15% glycerol 2 , Resuspend the precipitate;

[0146] 6) Dispense the...

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Abstract

The invention discloses a cotton GhACO gene and application of the cotton GhACO gene to plant flowering promotion, and belongs to the technical field of plant genetic engineering. The cotton GhACO gene has a nucleotide sequence shown as SEQ ID No. 3 and can encode an amino acid sequence shown as SEQ ID No. 4. Through a GhACO gene transformed arabidopsis thaliana plant obtained through a transgenictechnology, the number of rosette leaves is obviously reduced during bolting of arabidopsis thaliana; the flowering time is obviously advanced; and the result shows that the GhACO gene has a key effect on promoting cotton flowering. The invention provides a favorable gene resource for the cultivation of short-season cotton.

Description

Technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to the application of GhACO genes in promoting plant flowering. Background technique [0002] Cotton is an important economic crop. As an important raw material in textile, medicine, chemical and other fields, it occupies a very important position in my country's national economy. my country has a large population and small land, and grain and cotton are competing for land. In order to ensure food security, cotton planting area and output are also guaranteed. An effective way to solve this contradiction-early maturity cotton breeding. Early-maturing cotton varieties can realize the direct seeding of wheat (canola) in the cotton areas of the Yellow River and Yangtze River basins; it is beneficial to the improvement of quality and efficiency in the northwest inland cotton areas, and the safety of national cotton use. The early maturity of cotton is a compound...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/82A01H5/02A01H6/60A01H6/46A01H6/20
CPCC12N9/0071C12N15/827C12Y114/17004
Inventor 魏恒玲薛羽君喻树迅李林王寒涛马亮付小康
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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