Anti-aging wrinkle-removing nano-preparation as well as preparation method and use thereof

A nano-formulation, mixed solution technology, applied in the field of biomedicine, can solve problems such as poor safety and frequent side effects

Pending Publication Date: 2020-06-05
湖州浦瑞生物医药技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many skin care products use different chemicals as additives in order to pursue quick and direct results, resulting in poor safety and frequent side effects

Method used

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  • Anti-aging wrinkle-removing nano-preparation as well as preparation method and use thereof
  • Anti-aging wrinkle-removing nano-preparation as well as preparation method and use thereof
  • Anti-aging wrinkle-removing nano-preparation as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 prepares nano preparation

[0060] Nano preparations were prepared with reference to the method in the patent document CN1411368A. 8 g of hydroxypropyl tetrahydropyranotriol, 1.5 g of retinyl retinoate, and 0.5 g of soy isoflavone were dissolved in ethanol to obtain a total of 100 mL of a mixed solution. Dissolve 50 g of egg yolk lecithin in the mixed solution, heat to 40° C., and remove the ethanol solvent in the solution at 1330 Pa. The remaining liquid was added to 500ml PBS buffer solution and stirred to disperse.

[0061] The above-mentioned dispersion solution was emulsified using a high-pressure emulsifier, and the emulsified dispersion solution was sterilized by filtration through a 0.22 μm microporous membrane. Fill 15g of the filtrate into a lyophilized bottle, then put the lyophilized bottle into a lyophilizer, and operate at room temperature at 4000Pa for gas degassing. After degassing, concentrate at 40°C and 1330Pa to obtain a concentrate. ...

Embodiment 2

[0063] Example 2 Protective Effect on Human Skin Fibroblasts

[0064] Human skin fibroblasts (purchased from ATCC, product number: PCS-201-012), placed in ordinary DMEM medium (containing 10% (V / V) fetal bovine serum), in 5% CO 2 Cultivate in an incubator at 37°C. When 80% of the cells are confluent, subculture after trypsinization, and take the third generation of healthy cells for experiments.

[0065] After trypsinizing the cells, adjust the cell concentration to 5×10 4 cells / ml, inoculated in a 96-well plate and cultured until the cells adhered to the wall, discarded the culture medium, washed twice with PBS buffer, and placed under medium-wave ultraviolet light. 2 ), irradiate for 5min. After the ultraviolet irradiation is completed, add different cell culture medium to culture. In each experimental group, the nano-preparation A, nano-preparation B, nano-preparation C, nano-preparation D, and nano-preparation E prepared in Example 1 of 100 mg / L were added to the cell c...

Embodiment 3

[0070] Embodiment 3 promotes the expression test of mucopolysaccharide (hyaluronic acid) in vivo

[0071] 1. Experimental animals

[0072] Healthy male 6-8-week-old SPF mice with a body weight of (23±2) g. Raise under the environment of temperature (25 ± 2) ℃, humidity (40 ± 10)%.

[0073] 2. Establishment, grouping and administration of animal models

[0074] The mice were randomly divided into blank group, model group, matrix group, experimental group I, experimental group II, experimental group III and experimental group IV, with 6 mice in each group.

[0075] After the mice were adaptively fed for one week, they were depilated with 6% sodium sulfide, and the skin of 3cm×3cm was exposed. Except for the blank group, the other groups refer to the literature [ZHONG W, LIU N, XIE Y, et a1. Antioxidant and antiaging activities of mycelial polysaceharides from Lepista sordid[J]. Int J BiolMacromol, 2013, 60(6): 355-359 ] To establish a skin aging model, subcutaneously inject ...

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Abstract

The invention provides an anti-aging wrinkle-removing nano-preparation as well as a preparation method and use thereof. Specifically, the nano-preparation prepared by virtue of the preparation methodcontains hydroxypropyl tetrahydropyrantriol, retinyl retinoate and soy isoflavone as active ingredients and presents obvious anti-aging and wrinkle-removing effects.

Description

technical field [0001] The invention belongs to the field of biomedicine. Specifically, the invention relates to an anti-aging and wrinkle-removing composite nano-preparation and its preparation method and application. Background technique [0002] Skin aging includes endogenous skin aging and exogenous skin aging. Endogenous aging is determined by the genetic factors of the body and is a naturally occurring process that is difficult to prevent. It is manifested as dry skin, sagging, fine lines, sagging, thinning of the skin, and reduction of subcutaneous fat (causing sunken eye sockets and cheeks, etc.). Exogenous skin aging is the cumulative skin damage caused by environmental factors (ultraviolet rays, PM2.5, air pollution, bacterial infections, etc.) or lifestyle. Exogenous aging is not universal and can be prevented to a certain extent. It mainly occurs in exposed parts of the skin, such as the face, neck, back of the hands, and forearms, and manifests as deep wrinkles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/67A61K8/49A61K8/14A61Q19/08A61K31/35A61K31/352A61K31/215A61K9/127A61P17/18
CPCA61K8/671A61K8/498A61K8/14A61Q19/08A61K31/35A61K31/352A61K31/215A61K9/127A61P17/18A61K2800/413A61K2800/592A61K2800/5922A61K2800/78A61K2300/00
Inventor 魏维杨正茂熊江辉罗伯特·李
Owner 湖州浦瑞生物医药技术有限公司
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