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Blood calcium ion detection test paper

A technology of calcium ions and blood, which is applied in the direction of analyzing materials through chemical reactions, and analyzing materials through observing the influence of chemical indicators. Simple, Accuracy-Enhancing Effects

Pending Publication Date: 2020-06-05
SHAOXING PEOPLES HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, 8-hydroxyquinoline and other masking agents are commonly used to mask magnesium ions in blood, but different masking agents may have different masking effects on magnesium ions, and the masking effects of various masking agents on blood calcium determination are still to be studied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 The preparation of reagent composition 1 of the present invention

[0039] (1) Preparation of the first buffer solution: take the prescribed amount of formic acid, sodium chloride, sodium carbonate and sodium sulfate, add appropriate amount of distilled water, and prepare to contain 1.2ml / L formic acid, 14.5g / L sodium chloride, 6g / L carbonic acid The first buffer solution of sodium and 31.3g / L sodium sulfate;

[0040] (2) Prepare the first detection solution: add an appropriate amount of paraformaldehyde to the first buffer solution in step (1) to obtain the first detection solution containing 10g / L paraformaldehyde;

[0041] (3) Preparation of the second detection solution: get the prescription amount of o-cresolphthalein complex ketone, N-cyclohexyl-2-aminoethanesulfonic acid, N-benzoyl-N-phenylhydroxylamine, polyvinylpyrrolidone, polycyclic Oxyethylene lauric acid ether, Tween 20, diethylamine, adding appropriate amount of distilled water, prepared to c...

Embodiment 2

[0043] Embodiment 2 The preparation of reagent composition 2 of the present invention

[0044] The second buffer in Example 1 is replaced by N-cyclohexyl-2-aminoethanesulfonic acid with equimolar N-cyclohexyl-3-aminopropanesulfonic acid, and the remaining components and preparation process are the same as in Example 1 , to obtain the reagent composition 2 of the present invention.

Embodiment 3

[0045] Embodiment 3 The preparation of reagent composition 3 of the present invention

[0046] The masking agent in embodiment 1 is replaced by equimolar 8-hydroxyquinoline-5-sulfonic acid by N-benzoyl-N-phenylhydroxylamine, all the other components and preparation process are identical with embodiment 1, obtain Reagent composition 3 of the present invention.

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Abstract

The invention relates to a reagent composition, test paper and a kit for detecting the concentration of calcium ions in blood. The reagent composition comprises a first detection solution and a seconddetection solution, the first detection solution comprises paraformaldehyde and a first buffer solution, and the second detection solution comprises o-cresolphthalein complexing ketone, a second buffer agent, a surfactant, a masking agent and diethylamine. The reagent composition, the test paper and the kit are low in cost, simple to operate, rapid and accurate in detection result and high in sensitivity.

Description

technical field [0001] The invention relates to the field of reagent detection, in particular to a reagent for detecting calcium ion concentration. Background technique [0002] Calcium is one of the essential elements for the human body and one of the most abundant elements in the human body, and is closely related to various physiological activities. The abnormality (increase or decrease) of blood calcium is related to various diseases, such as hypercalcemia, hyperparathyroidism, hypoparathyroidism, multiple myeloma, bone malignancy, pancreatitis, etc. Therefore, the determination of blood calcium is of great significance. [0003] At present, the commonly used determination methods of blood calcium include complexometric titration and atomic absorption method. These methods require relevant instruments and equipment and specially trained testing personnel. The operation is complicated and the detection speed is slow. Moreover, these professional instruments and equipment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 徐潮阳陈国忠陈荣
Owner SHAOXING PEOPLES HOSPITAL
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