Santalum album and millet fermentation primary pulp cosmetics and preparation method and application thereof
A technology for fermenting puree and sandalwood, applied in the biological field, can solve the problems of skin irritation, difficult absorption and utilization of active ingredients, etc., and achieve the effects of preventing skin wrinkles, good market prospects, and preventing and controlling pigmented plaques.
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Embodiment 1
[0065] Embodiment 1: a kind of preparation method of sandalwood fermented protoplasm, specifically comprises the following steps:
[0066]The fermented puree of sandalwood is composed of 15 parts by weight of sandalwood, 5 parts by weight of millet, 120 parts by weight of purified water, 2-3 parts by weight of Saccharomyces cerevisiae and 2-3 parts by weight of Pichia pastoris.
[0067] Select Indonesian sandalwood and millet to grind, pass through a 30-mesh sieve after grinding, mix 15 parts by weight of sandalwood powder, 5 parts by weight of millet flour, 120 parts by weight of purified water, 2 parts by weight of Saccharomyces cerevisiae, and 2 parts by weight of Pichia pastoris. Cover and seal for 72 hours, keep the temperature at 30 degrees; stop fermentation at 72 hours, heat sterilization, sterilization temperature is 120 degrees, sterilization time is 60 minutes; centrifuge at 6000 rpm, discard the residue; save the supernatant for later use.
[0068] Above-mentioned ...
Embodiment 2
[0073] Embodiment 2: the application of Tanmi fermented puree as a skin care product.
[0074] 1. Properties of sandalwood fermented puree
[0075] The appearance of the fermented extract prepared in Example 1 was a viscous liquid and the color was pale white. The pH value is 5.2-6.3, the viscosity is 200-500cP, the soluble solid content is 1.5-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria are detected. According to the hygienic standard GB7916-87 of cosmetics, the total number of bacteria in cosmetics is not higher than 1000CFU / ml, so this fermented extract meets the quality requirements of cosmetics.
[0076] Carry out component analysis to this sandalwood fermented protoplasm, protein detection method refers to GB5009.5-2010; Crude polysaccharide detection method refers to GB / T 5009.8-2008; Flavone detection method refers to GB / T 5009.124-2003; Total phenol detection method refers to GB / T8313-2008 gives the following results:
[00...
Embodiment 1
[0079] The human patch test is mainly used to detect the irritation of cosmetic final products or raw materials. In the present invention, the fermented puree of sandalwood rice obtained in Example 1 is subjected to a human body closed patch test, aiming at evaluating its potential skin irritation.
[0080] 1. Test object
[0081] Choose 30 suitable volunteers, randomly selected in the age range of 18-60 years old.
[0082] 2. Test method
[0083] Weigh 0.020g-0.025g solid sample or semi-solid sample and put it into the spot tester for later use. Add 0.2mL to 0.025mL of the liquid sample dropwise on the filter paper, and then place the filter paper in the spot tester. Set a blank control for each sample, and add the same amount of sample solvent as the sample, such as distilled water or olive oil, into the hole of the control spot tester.
[0084] The test site is selected as the back of the human body, and the spot tester is fixed and pasted on the back of the subject wit...
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