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Method for simultaneously preparing polyphenols and polysaccharides from black currants

A black currant and polysaccharide technology, applied in the field of separation and purification of natural products, can solve the problems of incomplete extraction of polar active ingredients, cumbersome operation steps, waste of resources, etc., and achieve high resource utilization value, high extraction efficiency, and short time Effect

Active Publication Date: 2020-06-09
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are deficiencies in these two patents: (1) the extraction process first uses water as a solvent to extract the raw material 3-4 times, and then uses double water to separate the crude extract from the raw material to obtain polyphenols and polysaccharides. The method is time-consuming, the operation steps are cumbersome, and the active components of different polarities in the raw materials are not completely extracted, resulting in a waste of resources; while the present invention uses MA-ATPE to directly extract and separate polyphenols and polysaccharides from raw materials in one step, combining the advantages of MAE and ATPE , the extraction is more complete and the operation is simpler

Method used

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  • Method for simultaneously preparing polyphenols and polysaccharides from black currants
  • Method for simultaneously preparing polyphenols and polysaccharides from black currants
  • Method for simultaneously preparing polyphenols and polysaccharides from black currants

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A method for simultaneously preparing black currant polyphenols and polysaccharides, the steps are as follows:

[0038] (1) Add the black currant dry powder into the aqueous two-phase system composed of 30.47% ethanol and 18.02% ammonium sulfate according to the liquid-to-solid ratio of 50:1, and treat it for 8 minutes at the extraction temperature of 60°C and the extraction power of 600W . The upper and lower phase extracts were collected by centrifugation, and the volumes were measured respectively.

[0039] (2) Take the upper phase extract, wash with ethanol to remove ammonium sulfate, concentrate and freeze-dry to obtain the upper phase crude extract.

[0040] (3) The lower phase extract was taken and dialyzed for 48 hours at room temperature to remove inorganic salts and other small molecular impurities, the dialysate was concentrated and freeze-dried to obtain the lower phase crude extract.

[0041] (4) Dissolve the crude extract of the upper phase, load the sam...

Embodiment 2

[0059] A method for simultaneously preparing black currant polyphenols and polysaccharides, the steps are as follows:

[0060] (1) Add the black currant dry powder into the aqueous two-phase system composed of 30.47% ethanol and 18.02% ammonium sulfate according to the liquid-to-solid ratio of 50:1, and treat it for 10 minutes at the extraction temperature of 60°C and the extraction power of 500W . The upper and lower phase extracts were collected by centrifugation, and the volumes were measured respectively.

[0061] (2) Take the upper phase extract, wash with ethanol to remove ammonium sulfate, concentrate and freeze-dry to obtain the upper phase crude extract.

[0062] (3) The lower phase extract was taken and dialyzed at room temperature for 48 hours to remove inorganic salts and other small molecular impurities, the dialysate was concentrated and freeze-dried to obtain the lower phase crude extract.

[0063] (4) Dissolve the crude extract of the upper phase, load the sa...

Embodiment 3

[0068] A method for simultaneously preparing black currant polyphenols and polysaccharides, the steps are as follows:

[0069] (1) Add the black currant dry powder into the aqueous two-phase system composed of 30.47% ethanol and 18.02% ammonium sulfate according to the liquid-to-solid ratio of 60:1, and treat it for 8 minutes at the extraction temperature of 65°C and the extraction power of 600W . The upper and lower phase extracts were collected by centrifugation, and the volumes were measured respectively.

[0070] (2) Take the upper phase extract, wash with ethanol to remove ammonium sulfate, concentrate and freeze-dry to obtain the upper phase crude extract.

[0071] (3) The lower phase extract was taken and dialyzed at room temperature for 48 hours to remove inorganic salts and other small molecular impurities, the dialysate was concentrated and freeze-dried to obtain the lower phase crude extract.

[0072] (4) Dissolve the crude extract of the upper phase, load the sam...

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Abstract

The invention relates to a method for simultaneously preparing polyphenols and polysaccharides from black currants. The method comprises the following steps: black currant fruit dry powder is extracted by microwave-assisted ethanol-ammonium sulfate dual aqueous phase system in one step, then separated to obtain two phases. The upper phase is an ethanol phase containing a large amount of polyphenols and a small amount of polysaccharides, and the lower phase is an inorganic salt phase rich in another polysaccharide. In the upper phase, the polyphenols are separated from the polysaccharides by anAB-8 macroporous resin column, and then the polysaccharides and the polyphenols are eluted sequentially with acidic water and ethanol aqueous solution. The two eluents are collected, concentrated, and lyophilized to obtain polysaccharides and polyphenols in the upper phase. The polysaccharides in the lower phase is obtained by dialysis, alcohol precipitation, and lyophilization. The microwave-assisted dual aqueous system constructed by the invention improves the extraction efficiency and separation effect of polyphenols and polysaccharides from the black currants, and simultaneously preparesthree active ingredients, thereby greatly improving the utilization rate of raw materials. The method of the present invention has mild conditions, low cost, and simple process operation without causing inactivation or denaturation of polyphenols and polysaccharides from black currants, and is easy to use in industrial production.

Description

Technical field: [0001] The invention relates to a method for separating and purifying natural products, in particular to a method for simultaneously preparing black currant polyphenols and polysaccharides. Background technique: [0002] Black currant (Ribes nigrum L.), also known as black currant, is a small perennial deciduous shrub belonging to the order Saxifragae Curamaceae. Black currant is rich in nutrition and has high edible and medicinal value. It can be eaten directly or processed into jam, fruit wine and fruit juice. Polysaccharide is an important active ingredient in black currant fruit, which has attracted much attention because of its biological activities such as anti-oxidation, anti-tumor, anti-fatigue, and hypoglycemia. In addition, black currant fruit is rich in a large amount of polyphenols (including flavonoids, anthocyanins, phenolic acids, etc.), which have obvious biological activities such as antioxidant, anti-inflammatory, antibacterial, and antica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A23L33/105A23L33/125
CPCA61K36/185A23L33/105A23L33/125A61K2236/333A61K2236/53A61K2236/55A61K2236/51A23V2002/00A23V2200/30A23V2250/2132A23V2250/51
Inventor 杨昱赵梅梅白靖文徐雅琴王丽波
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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