Method for detecting content of aldehyde groups contained in biological tissue
A technology for the content of biological tissue and aldehyde groups, applied in the detection field, can solve the problems of inability to detect products, affect the content of free aldehyde groups, and many reaction steps, and achieve the effects of rapid detection, high detection accuracy and simple operation
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[0044] Example 1
[0045] (1) Establish a standard curve
[0046] Precisely prepare 0.1mmol / L DNPH solution, in which acetic acid is used to adjust the pH of the DNPH solution to 6.0.
[0047] Select 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 7ml, 8ml, 9ml, 10ml of the DNPH solution with a concentration of 0.1mmol / L, dilute to 10ml with purified water, and use an ultraviolet spectrophotometer to measure the DNPH solution at 218nm. For the absorbance value, draw the standard curve with the concentration of the DNPH solution as the abscissa and the absorbance as the ordinate. The standard curve obtained is as figure 1 As shown, the regression equation of the curve is: y=10.248x+0.0273, R2=0.9991, where x represents the concentration of the DNPH solution, the unit is mmol / L, and y represents the absorbance value.
[0048] (2) Detection of the content of aldehyde groups contained in biological tissues
[0049] Take 5ml of 0.1mmol / L DNPH solution and use an ultraviolet spectrophotometer to test that th...
Example Embodiment
[0053] Example 2
[0054] (1) Establish a standard curve
[0055] Precisely prepare 0.3mmol / L DNPH solution, in which phosphoric acid is used to adjust the pH of the DNPH solution to 2.0.
[0056] Select 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 7ml, 8ml, 9ml, 10ml of the DNPH solution with a concentration of 0.1mmol / L, dilute to 10ml with purified water, and use an ultraviolet spectrophotometer to measure its DNPH solution at 264nm For the absorbance value, draw the standard curve with the concentration of the DNPH solution as the abscissa and the absorbance as the ordinate. The standard curve obtained is as figure 1 As shown, the regression equation of the curve is: y=10.293x+0.0227, R 2 =0.9998, where x represents the concentration of the DNPH solution in mmol / L, and y represents the absorbance value.
[0057] (2) Detection of the content of aldehyde groups contained in biological tissues
[0058] Dilute 10ml of 0.3mmol / L DNPH solution to 20ml as a DNPH reaction solution, and use an ultraviole...
Example Embodiment
[0064] Example 3
[0065] (1) Establish a standard curve
[0066] Precisely prepare a 1.0mmol / L DNPH solution, wherein perchloric acid is used to adjust the pH of the DNPH solution to 4.0.
[0067] Select 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, 7ml, 8ml, 9ml, 10ml of the DNPH solution with a concentration of 1.0mmol / L respectively, dilute to 10ml with purified water, and use an ultraviolet spectrophotometer to measure the DNPH solution at 323nm For the absorbance value, draw the standard curve with the concentration of the DNPH solution as the abscissa and the absorbance as the ordinate. The standard curve obtained is as figure 1 As shown, the regression equation of the curve is: y=10.329x+0.034, R2=0.998, where x represents the concentration of the DNPH solution, the unit is mmol / L, and y represents the absorbance value.
[0068] (2) Detection of the content of aldehyde groups contained in biological tissues
[0069] Dilute 1ml of 1.0mmol / L DNPH solution to 10ml, and use an ultraviolet spectro...
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