Method for determining content of carotenoid in red-heart duck egg

Abstract

The invention discloses a method for determining the content of carotenoid in a red-heart duck egg and belongs to the field of chromatographic detection. The method comprises the following steps: extracting a sample by acetonitrile; degreasing with normal hexane after concentrating; and adopting an Agilent Poroshell 120 EC-C18 (4 micrometers, 4.6 mm*100 mm) chromatographic column for separation, wherein 20 mmol / L ammonium acetate and acetonitrile are used as mobile phases for gradient elution, the flow rate of the mobile phases is controlled to be 1.0 mL / min, the detection wavelength of a diode array detector is 471 nm, and the content of the carotenoid in the red-heart duck egg can be measured within 25 min. The method is simple to operate, good in separation degree and high in sensitivity and accuracy, and provides a method reference for determining the content of the carotenoid in the red-heart duck egg.

Description

technical field [0001] The invention belongs to the field of chromatographic detection, and relates to a method for determining the content of carotenoids in red duck eggs, in particular to a high-performance liquid chromatography method for determining the content of carotenoids in red duck eggs. Background technique [0002] With the continuous improvement of people's living standards, people's requirements for food are not only in terms of nutritional needs, but also have a higher pursuit of visual needs. Egg yolk coloring is a natural process, and carotenoids are the main coloring factors that form egg yolk. Since birds cannot synthesize these carotenoids themselves, they need to be obtained from the food they eat and deposited into the yolk. Therefore, in the feed Adding different proportions of such pigments can change the color of the egg yolk to attract consumers. However, there are still safety risks due to the use of carotenoids, and studies in recent years have s...

AI Technical Summary

Problems solved by technology

Due to the unstable nature of carotenoids, it is easily affected by light, heat, acid, alkali and other environments, which makes it more difficult to determine the content of carotenoids in duck eggs.

At present, the main analysis method of carotenoids is high performance liquid chromatography (HPLC), but there is no report on the simultaneous determination of multiple carotenoids in eggs, and the domestic detection method of carotenoids is mainly through column chromatography. , organic reagent extraction, saponification and other methods for sample pretreatment, the steps are cumbersome, and a large amount of volatile toxic reagents such as acetone, petroleum ether, chloroform, ethyl acetate, etc. are required, which is harmful to the testing personnel

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