Method and test strip for rapid detection of new coronavirus nucleic acid recombinase mediated isothermal amplification lateral chromatography colloidal gold

Abstract

The invention relates to a test strip for rapid detection of new coronavirus nucleic acid recombinase mediated isothermal amplification lateral chromatography colloidal gold. The test strip comprisesa lining plate, and a sample pad, a gold-labeled combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially connected to the lining plate, and a detection line and aquality control line are arranged on the nitrocellulose membrane; a goat anti-mouse antibody is sprayed on the quality control line, and streptavidin is sprayed on the detection line; and a colloidalgold labeled mouse anti-isothiocyanate fluorescein antibody is sprayed on the gold labeled combination pad. An amplification product obtained by enzyme-mediated isothermal amplification of a sample isdropwise added to the sample pad, and a detection result can be obtained according to a color reaction. The detection result is judged according to whether the detection line on the colloidal gold test strip develops color or not, a complex professional background is not needed, and the detection result can be judged by naked eyes.

Description

technical field [0001] The invention belongs to the technical field of virus detection, and relates to a colloidal gold rapid detection method and a test strip mediated by isothermal amplification lateral flow chromatography of the new coronavirus nucleic acid recombinase. Background technique [0002] At present, the nucleic acid detection of the new coronavirus is the only way to diagnose cases. Real-time fluorescent quantitative RT-PCR technology is widely used. There are many manufacturers of detection reagents approved, and the production capacity can meet the actual needs, but this method requires BSL-2 level experiments Harsh conditions and expensive equipment such as laboratory, real-time fluorescent quantitative PCR instrument and nucleic acid extractor, and the detection cycle is long (3-4 hours), and the cost of reagent consumables is high. At present, it can only be carried out in some municipal CDCs and tertiary medical institutions, and the detection capacity c...

AI Technical Summary

Problems solved by technology

[0002] At present, the nucleic acid detection of the new coronavirus is the only way to diagnose cases. Real-time fluorescent quantitative RT-PCR technology is widely used. There are many manufacturers of detection reagents approved, and the production capacity can meet the actual needs, but this method requires BSL-2 level experiments Harsh conditions and expensive equipment such as laboratory, real-time fluorescent quantitative PCR instrument and nucleic acid extractor, and the detection cycle is long (3-4 hours), and the cost of reagent consumables is high

At present, it can only be carried out in some municipal CDCs and tertiary medical institutions, and the detection capacity cannot meet the actual needs. There is an urgent need for a nucleic acid detection method that can be operated in ordinary laboratories, does not require high-end equipment, rapid detection, and direct interpretation.

Images