Efficient electron carrier screening, designing and modifying method

Abstract

The invention discloses an efficient electron carrier screening, designing and modifying method, and belongs to the field of bioelectrochemical catalysis. According to the invention, Shewanella oneidensis is used as an experimental object to screen and modify an electron carrier so as to improve the extracellular electron transfer capability of the electron carrier. In order to screen out electroncarriers with excellent performance, a single-chamber bioelectrochemical system and a double-chamber bioelectrochemical system are established, a microbial extracellular electron transfer detection method is established and perfected, and evaluation indexes such as cytotoxicity, current density, reduction equivalent and coulombic efficiency are determined. In order to further improve the performance of the electron carrier, the anthraquinone-2-sodium sulfonate (AQS) electron carrier is reasonably designed and modified based on a density functional theory, and seven substituent group positionsand six side chain groups are selected for theoretical calculation of standard Gibbs free energy change and oxidation-reduction potential. Experimental analysis proves that the method for reducing the redox potential of the electron carrier through design and transformation is an effective method for improving the extracellular electron transfer capacity of electroactive microorganisms.

Description

technical field [0001] The invention relates to a method for screening, designing and transforming high-efficiency electron carriers, belonging to the field of bioelectrochemical catalysis. Background technique [0002] At present, environmental and energy issues have become the core issues affecting human survival and development. With the use of fossil energy, we are facing a huge energy crisis and environmental pollution. How to balance the environment and energy needs and achieve sustainable development is a frontier and hot issue we are currently facing. In recent years, bioelectrochemical system (BES), as an emerging technology to effectively recover energy and resources, has received extensive attention and has great potential for development. The most common form of bioelectrochemical systems is microbial fuel cells for electricity generation or sewage treatment, but bioelectrochemical systems also show broad prospects for product synthesis and bioremediation [1] ....

AI Technical Summary

Problems solved by technology

[0005] In view of the fact that there are still many problems with electron carriers, such as the large difference in the efficiency of different electron carriers, most electron carriers have cytotoxicity to microorganisms, and electron carriers mediate the cytotoxicity of microorganisms The mechanism of exoelectron transfer is not yet clear, which severely limits the development of microbial electrosynthesis.

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