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A kind of breeding method of colored rice two-line hybrid rice

A technology of hybrid rice and colored rice, which is applied in the field of breeding of two-line hybrid rice of colored rice, can solve the problems of restricting production and consumer application, no advantage in yield and quality, and decline in functional nutrition, so as to reduce the risk of production promotion, Shorten the breeding time, the effect of beautiful color

Active Publication Date: 2021-12-17
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

For the above-mentioned varieties, whether they are red rice or black rice hybrid varieties, the sterile lines are common non-colored rice, and only the restorer lines are red rice or black rice. Restricted by genetic characteristics, the seed coats of these varieties are not obvious enough. For example, the brown rice of red rice hybrid rice is reddish-brown, atypical red; the brown rice of black rice hybrid rice is black or dark brown. Although the yield and resistance are higher than those of conventional colored rice varieties, the functional nutrition has decreased. , compared with hybrid rice super varieties, there is no advantage in yield and quality, which restricts the application of production and consumers

Method used

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  • A kind of breeding method of colored rice two-line hybrid rice

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Embodiment 1

[0042] A method for breeding colored rice two-line hybrid rice, the specific steps are as follows:

[0043] (1) Population construction: Indica black rice line Zhongze Heizhen (ie: colored rice +C ) as the male parent, Guangzhan 63-4S (i.e. two-line male sterile line S) as the female parent, and the offspring obtained were backcrossed with Guangzhan 63-4S for 4 times to obtain BC 4 f 1 2 generation plants, BC 4 f 1 Generation selfing 1 time, harvest BC 4 f 2 substitute seeds;

[0044] (2) Select test cross: observe BC 4 f 2 A total of 1202 generation seeds were selected, and homozygous seeds with colored seed coats were selected and planted as a single plant, and a total of 322 plants were planted; when planting to flowering, 53 male sterile plants among the 322 plants were selected to obtain the male sterile plant S ' 1 ; The male sterile plant S' 1 Paired test crossing with Yangdao 6 (i.e. restorer line R) to obtain hybrid F 1 generation;

[0045] Then 53 male st...

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Abstract

The invention discloses a method for breeding two-line hybrid rice of colored rice. The method comprises: using colored rice +C as the male parent, and the two-line sterile line S as the female parent, cross and backcross with the female parent to obtain BC n f 2 Generation seeds; select male sterile plants, testcross with the two-line restorer line R in pairs, select homozygous sterile line plants with colored seed coats and corresponding hybrid seeds; detect heterosis of seeds, and select hybrid seeds with heterosis Hybridize plants and CMS plants to obtain CMS seeds of colored rice; identify the fertility, yield, quality and resistance of CMS to obtain excellent CMS plants of colored rice; multiply seeds to obtain two lines of colored rice hybrid system. In the present invention, the colored rice is crossed and backcrossed with the approved two-line sterile line of hybrid rice, and the two-line sterile line with colored seed coat and heterosis is screened through the restorer line to obtain the colored rice hybrid rice and its CMS not only has colored seed coat, but also has the advantages of high yield, high quality and disease resistance.

Description

technical field [0001] The invention relates to the technical field of rice breeding, in particular to a breeding method for two-line hybrid rice of colored rice. Background technique [0002] Colored rice refers to the color of brown rice, mainly black, purple, red, yellow, green and so on. Colored rice is brown rice because it contains seed coat, rice germ and aleurone layer, so its protein, fat, amino acid, vitamin, mineral trace element content is significantly higher than that of white rice (polished rice) eaten daily, and it is rich in natural pigments And a variety of physiologically active substances, it is very consistent with the traditional food culture of "both food and medicine" in our country. For example: in the folk, black rice is called "longevity rice", and there is a saying that "when it is black, it must be nourished"; red rice is often used to "enrich blood", which can nourish yin and kidney, invigorate the spleen and appetizer, and is a health food for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 吴殿星章思妍陈霄雨
Owner ZHEJIANG UNIV
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