Application of metabolite of metarhizium anisopliae in preparation of gray mold biocontrol preparation

A technology of Metarhizium anisopliae and metabolites, which is applied in the directions of plant growth regulators, applications, biocides, etc., can solve the problems of no inhibition, weak botrytis antagonism, etc., and achieve the effect of easy preservation

Pending Publication Date: 2020-06-16
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the report found that Metarhizium anisopliae has antagonistic properties to Botrytis cinerea, it also pointed out that the biocontrol bacteria with better antagonistic effects screened out by the plate confrontation method are not necessarily the best biocontrol factors in practical applications. It will be interfered by the environment and other non-environmental factors, so although the Metarhizium anisopliae screened out has antibacterial effect on the plate, it is not known whether it is suitable for crop biocontrol
[0005] Existing reports have confirmed that not all Metarhizium anisopliae have the effect of inhibiting Botrytis cinerea. Yun et al. (2017) discussed the antagonistic effect of Metarhizium anisopliae strain SD3 on Botrytis cinerea. About 1mm, indicating that the antagonistic effect of the strain on Botrytis cinerea is very weak, which can be regarded as no inhibitory effect in this field

Method used

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  • Application of metabolite of metarhizium anisopliae in preparation of gray mold biocontrol preparation
  • Application of metabolite of metarhizium anisopliae in preparation of gray mold biocontrol preparation
  • Application of metabolite of metarhizium anisopliae in preparation of gray mold biocontrol preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Antagonism of Metarhizium anisopliae IBCCM321.93 to Grape Cinerea

[0034] Prepare PDA medium according to the conventional method, cut each 200g of potatoes into small pieces and cook, filter and collect the filtrate and add 20g of glucose, add water until the total volume is 1000ml, which is the PDB medium; then subpackage, add 1.5 g of agar was prepared into PDA medium after high-temperature steam sterilization.

[0035]Metarhizium anisopliae IBCCM321.93 (strain preservation number is CGMCC No.0853) was inoculated at one end of the PDA medium (petri dish diameter 9 cm) and cultivated at 28°C for 2 days at the edge, then inoculated at the edge of the other end of the medium Botrytis cinerea strain B05.10. Botrytis cinerea was only inoculated at one end of the petri dish as a control. After continuing to culture for 4 days, it was observed that an antagonistic band of about 6 mm was formed between the two strains. According to the formula inhibitory rate=...

Embodiment 2

[0036] Example 2: The fermentation filtrate of Metarhizium anisopliae IBCCM321.93 inhibits Botrytis cinerea mycelium growth and sclerotia formation

[0037] Metarhizium anisopliae IBCCM321.93 was inoculated in 100 ml of PDB medium, shaken at 160 rpm, and cultured at 28° C. for 10 days. Centrifuge at 10,000rpm for 15min to remove the mycelia, then filter the supernatant with a 0.22 μm bacterial filter, collect the filtrate as the fermentation filtrate stock solution of Metarhizium anisopliae, and use it in the following examples.

[0038] Add the fermentation filtrate of Metarhizium anisopliae to the PDA medium until the concentration of the fermentation filtrate of Metarhizium anisopliae is 5% (v / v), and then inoculate Botrytis cinerea. Botrytis cinerea was inoculated on PDA medium without fermentation filtrate as a control. After 4 days of cultivation, the colonies of Botrytis cinerea growing on the medium containing the fermentation filtrate were significantly smaller than ...

Embodiment 3

[0039] Embodiment 3: the fermentation filtrate of Metarhizium anisopliae IBCCM321.93 inhibits the sclerotia germination of Botrytis cinerea

[0040] After surface disinfection of the sclerotium of Botrytis cinerea, soak in the diluted Metarhizium anisopliae fermentation filtrate (concentration is 10% of the fermentation filtrate stock solution of Metarhizium anisopliae, v / v) for 24 hours. Sclerotia soaked in sterile water was used as a control. The soaked sclerotia were dried and placed on PDA for 2 days at 20°C, and the number of germinated sclerotia was recorded. The results showed that about 96.89% of the sclerotias germinated in the control group, and about 35.04% of the sclerotias germinated after being treated with the fermentation filtrate of Metarhizium anisopliae. It shows that the fermentation filtrate of Metarhizium anisopliae has inhibitory effect on the sclerotia germination of Botrytis cinerea ( image 3 ).

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Abstract

The invention belongs to the technical field of biological control of plant diseases, and particularly relates to an application of a metabolite of metarhizium anisopliae in preparation of gray mold biocontrol agents. The used metarhizium anisopliae IBCCM321.93 has significant inhibition on mycelial growth, sclerotium formation, sclerotium germination, spore germination and the like of botrytis cinerea. Fermentation filtrate of the strain has prevention and treatment effects on gray mold on plants and gray mold on picked fruits. In addition, volatile gas generated by the metarhizium anisopliaealso has a good prevention and treatment effect on gray mold on fruits in the storage period.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to the application of metabolites of Metarhizium anisopliae IBCCM321.93 in the preparation of gray mold biological control preparations. [0002] technical background [0003] Botrytis cinerea caused by Botrytis cinerea is a worldwide distribution of plant pathogenic fungi. Botrytis cinerea has a wide range of hosts and can harm more than 200 kinds of plants, including tomatoes, cucumbers, strawberries, grapes and many other economic crops, causing serious economic losses. The sclerotia and conidia produced by Botrytis cinerea play an important role in the disease cycle of Botrytis cinerea, so inhibiting the formation or germination of sclerotia and conidia can effectively control Botrytis cinerea. At present, the control of Botrytis cinerea is still dominated by chemical control, but it has been reported that Botrytis cinerea resistant to mu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01P3/00A01G13/00A23B7/155
CPCA01G13/00A23B7/155A23V2002/00A23V2200/10
Inventor 肖雪琼肖炎农王高峰辛西娅·萨文谢冲郝秋燕黄求应
Owner HUAZHONG AGRI UNIV
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