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Enrichment method of electroactive biofilms for antibiotic wastewater treatment

A technology of antibiotic wastewater and biofilm, applied in biological water/sewage treatment, water/sludge/sewage treatment, electrochemical biological combination treatment, etc., can solve the problems of poor tolerance and long domestication cycle of electrode biofilm, and achieve Solve the difficulty of domestication, shorten the domestication cycle, and improve the effect of activity

Active Publication Date: 2022-07-08
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the shortcomings and deficiencies of the prior art, the object of the present invention is to provide a method for rapid enrichment of electroactive biofilms that can be used for high-concentration antibiotic wastewater treatment, which solves the problem of electrode biofilm domestication in bioelectrochemical treatment of antibiotic wastewater. The problem of long cycle and poor tolerance has broad application prospects

Method used

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  • Enrichment method of electroactive biofilms for antibiotic wastewater treatment
  • Enrichment method of electroactive biofilms for antibiotic wastewater treatment
  • Enrichment method of electroactive biofilms for antibiotic wastewater treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0043] This example illustrates the influence of the synchronous domestication and the traditional sequential domestication method adopted in the present invention when using the MFC anode to domesticate the electroactive biofilm degraded by chloramphenicol:

[0044] build as figure 1 In the dual-chamber MFC shown, a proton exchange membrane is arranged between the anode compartment and the cathode compartment, and an anode electrode and a cathode electrode are respectively arranged, the anode electrode and the cathode electrode are connected by an external circuit, and the cathode or anode material is a carbon-based material.

[0045] MFC anode uses anaerobic activated sludge (taken from Nanjing Jiangxinzhou Sewage Treatment Plant, hereinafter referred to as sludge) as the source of inoculated bacteria, the inoculation ratio is 1:2 (sludge: anolyte), and the anolyte is COD=1000mg / L The glucose simulated organic wastewater (0.31g / LNH 4 Cl; 2.452g / LNaH 2 PO 4 ·H 2 O; 4.576g...

Embodiment 2

[0049] This example illustrates that when the electroactive biofilm degraded by chloramphenicol is acclimated at the anode of the MFC using a new synchronous acclimation method, the sludge, sludge leaching solution, and sterilized sludge will be further investigated after the MFC has stabilized electricity production. Influence of leaching solution and sterilized sludge:

[0050] build as figure 1 The dual-chamber MFC shown, the new synchronous domestication method is as described in Example 1, and chloramphenicol is added to the anode gradient at the beginning, and the anolyte and sludge are regularly replaced every 3 days. When the dosing concentration was 5 mg / L, the battery produced electricity for 2 consecutive cycles and tended to be stable. In the later stage, different experimental groups were added with sludge, sludge extract, sterilized sludge extract, and sterilized sludge, respectively. The added concentration of chloramphenicol was still increasing gradually, and...

Embodiment 3

[0053] This example illustrates the acclimation of electroactive biofilms of different antibiotic wastewaters in MFC anodes using simultaneous acclimation and traditional sequential acclimation:

[0054] build as figure 1 The two-chamber MFC shown, the novel simultaneous acclimation versus the traditional sequential acclimation approach is as described in Example 1. Antibiotics were selected as typical representatives of 6 classes of antibiotics: chloramphenicol, tetracycline, sulfamethoxazole, penicillin, metronidazole, and nitrofurazone. As shown in Table 1, after 30 days of MFC operation in all experimental groups, the degradation of the target antibiotics was measured every 72 hours of operation. The tolerance concentration of chloramphenicol was only 15mg / L, and the degradation rate was 89.2%. Under the synchronous acclimation mode, the tolerated concentration of tetracycline can reach 20 mg / L, and the degradation rate is 100%; under the sequential acclimation method, t...

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Abstract

The invention provides a rapid enrichment method for electroactive biofilms used for antibiotic wastewater treatment. A double-chamber MFC device is used to add anolyte containing antibiotics and anaerobic activated sludge to the anode chamber of the MFC device, and the concentration of the antibiotics Dosing increases gradually, and the volume ratio of anaerobic activated sludge to anolyte is 1:2 each time. Decrease gradually until it reaches 0, and replace the anolyte and catholyte regularly. This method can effectively improve the domestication efficiency of MFC anode electroactive biofilms used to treat antibiotic wastewater. Compared with the traditional domestication method, the domestication period is significantly shortened (2.78 times) and the tolerance to antibiotics is increased (2.67 times). It is suitable for the domestication of electrode biofilms for various typical antibiotic wastewater treatment, which solves the problems of long domestication period and poor tolerance of electrode biofilms in bioelectrochemical treatment of antibiotic wastewater, and has broad application prospects.

Description

technical field [0001] The invention belongs to the fields of biological energy and electrochemistry, and in particular relates to a rapid enrichment method for electroactive biofilms used for high-concentration antibiotic wastewater treatment. Background technique [0002] Antibiotics have been widely used in human medicine and animal husbandry since the 1950s. A large number of antibiotics enter the water environment in the form of original drugs or metabolites through human and animal excrement, agricultural and pharmaceutical industry wastewater discharge, causing great pollution. Bioelectrochemical system is an emerging technology that utilizes microorganisms to catalyze anodic (oxidation) or cathodic (reduction) reactions, mainly including microbial fuel cells (MFCs) and microbial electrolysis cells (MECs). Anaerobic biotechnology and electrochemical technology have stronger pollutant degradation ability, and they have the advantages of high efficiency, low cost, envi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/00C02F3/28C02F101/30
CPCC02F3/005C02F3/2813C02F2101/30Y02E60/50
Inventor 吴夏芫陈子璇贾红华李春蕊张丽娜崔衍潘正勇陈雪茹韦萍
Owner NANJING TECH UNIV
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