HDN staining solution and kit for specific color development of horseradish peroxidase by using HDN staining solution

Abstract

The invention belongs to the technical field of cell staining and kits, and particularly relates to an HDN staining solution and a kit thereof for specific color development of horseradish peroxidaseby using the HDN staining solution. The kit comprises the HDN staining solution, wherein the HDN staining solution comprises hydrogen peroxide, a buffer solution, ammonium nickel sulfate, 3, 3'-diaminobenzidine and ammonium chloride. An immunohistochemistry method of the kit comprises the following steps of: (1) slicing and fixing a detected living tissue; 2) detecting a to-be-discovered antigen or antibody by using a primary antibody; 3) carrying out staining, specifically, immersing the obtained tissue slice into the HDN staining solution, wherein a positive detection object of the tissue slice is dark purplish blue; 4) cleaning the tissue slice; 5) and sealing the slice, and observing the tissue slice. By adopting the HDN staining solution for staining, the obtained sensitivity of the kit is more than 10 times higher than the sensitivity obtained by using the conventional method, the substance which cannot be stained by using the conventional method can be displayed, the false negative result can be reduced, the missed diagnosis can be avoided, and the false positive result can be avoided since the non-positive substance is not stained.

Description

technical field [0001] The invention belongs to the technical field of cell staining and kits, and in particular relates to an HDN staining solution and a kit for horseradish peroxidase-specific color development. Background technique [0002] Immunohistochemistry or immunocytochemistry is a technique that uses labeled antibodies (or antigens) to display the distribution and properties of antigens or antibodies, proteins, nucleic acid molecules, neurotransmitters, and receptors in animal and plant tissues in situ. It can be used to detect changes caused by injuries, trace nerve connections, detect pathogens and poisons, etc. It has high specificity for antigen-antibody binding and accuracy for in-situ tissue display. [0003] Since the first successful application of immunohistochemical technology in 1941, it has developed rapidly. Enzyme-labeled antibody technology, such as PAP, ABC, SPA and other technologies, and immuno-electron microscopy technology have been established...

AI Technical Summary

Problems solved by technology

[0004] Horseradish peroxidase (HRP) is a commonly used labeling enzyme, which is mainly used in the biological and medical fields to detect proteins, antibodies, antigens, pathogens, viruses and other substances. Only when it presents a color can it display the marked substance. The DAB staining method commonly used in the prior art uses DAB staining solution to stain horseradish peroxidase, which has the problem of insensitivity, and the specificity is not strong, and the background of the stained tissue is very large. Deep, there will be false negative and false positive results; while using traditional immunofluorescence staining, the fluorescence will be weakened due to the time relationship, and it will fade while observing under the microscope, which is not easy to observe, and cannot be preserved for review, let alone Electron Microscopic Observation of Ultrastructure

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