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Application of fermented clear liquid of lactobacilli in oxidation resisting or preparation of products for oxidation resisting and oxidation resisting product

A technology for fermenting serum and Lactobacillus, which is applied in the field of microbiology and can solve problems such as the lack of research on the antioxidant properties of Lactobacillus fermented serum

Pending Publication Date: 2020-06-19
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the current research on the antioxidant properties of Lactobacillus is limited to the research on the lactic acid bacteria itself, and there is no research on the antioxidant properties of the fermented serum of Lactobacillus

Method used

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  • Application of fermented clear liquid of lactobacilli in oxidation resisting or preparation of products for oxidation resisting and oxidation resisting product
  • Application of fermented clear liquid of lactobacilli in oxidation resisting or preparation of products for oxidation resisting and oxidation resisting product

Examples

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preparation example Construction

[0018] According to the present invention, the preparation method of the lactobacillus fermentation liquid comprises: inoculating the lactobacillus into the culture liquid to carry out fermentation culture, thereby obtaining the lactobacillus fermentation liquid.

[0019] Wherein, the inoculum amount of the lactobacillus can be changed within a wide range, preferably, the inoculum amount of the lactobacillus is 1-3% by volume.

[0020] Preferably, before inoculating the lactobacillus into the culture medium, it also includes activating the lactobacillus, and the activation can be performed according to a conventional method in the art, for example, inoculating the lactobacillus stored at low temperature into the Cultivate (for example, 8-15 hours) in the above-mentioned culture medium. The number of activation generations can be 1-3 times.

[0021] According to the present invention, the culture solution can be a conventional liquid medium for the cultivation of lactic acid b...

preparation example

[0041] This preparation example is used to illustrate the activation of bacterial strains

[0042] The strains preserved in glycerol tubes of Lactobacillus salivarius M18-6 and Lactobacillus rhamnosus LGG frozen in -80°C ultra-low temperature refrigerator were inoculated into MRS liquid medium at an inoculation amount of 2% by volume, and then inoculated at 37 ℃, 180rpm, cultured for 12h, after continuous activation for 3 generations, the follow-up experiment was carried out.

Embodiment 1

[0044] This example is used to illustrate the preparation of Lactobacillus fermentation serum

[0045] The activated Lactobacillus salivarius M18-6 in the preparation example was inoculated into the MRS liquid medium 1 with an inoculation amount of 2% by volume, and then cultured at 37° C. and 180 rpm for 12 hours to obtain a fermentation broth.

[0046] The fermented fermentation liquid was centrifuged at 6000 rpm at 4°C for 10 min, and the supernatant collected was the fermentation supernatant required for the experiment.

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Abstract

The invention relates to the field of microbes and particularly relates to application of fermented clear liquid of lactobacilli in oxidation resisting or preparation of products for oxidation resisting and an oxidation resisting product. The oxidation resisting product contains the fermented clear liquid of the lactobacilli. Discovered through researches, the fermented clear liquid of the lactobacilli has relatively high oxidation resistance, for example, capacity for clearing hydroxyl radicals, capacity for inhibiting overoxidation of linoleic acid and activity for resisting oxidase substances. Furthermore, discovered through the researches, compared with other lactobacilli, lactobacillus salivarius, particularly lactobacillus salivarius CGMCC No. 16199 has higher oxidation resistance.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application of lactobacillus fermented serum in anti-oxidation or preparation of anti-oxidation products and anti-oxidation products. Background technique [0002] A large number of studies have shown that lactic acid bacteria have a variety of probiotic functions, not only can regulate the body's immune system, maintain the balance of intestinal flora, but also have a certain antioxidant capacity, can remove active oxygen molecules in the intestinal tract, and maintain the active oxygen molecules in the body. in a relatively stable state. [0003] Oxidative stress is the root cause of aging and aging-related diseases. Oxidative stress may cause a variety of diseases, such as diabetes, atherosclerosis, arthritis, hyperlipidemia, cardiovascular and cerebrovascular diseases, etc. In normal biological cells, there is an antioxidant defense system including antioxidant enzymes and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P39/06A61K8/99A61Q19/08A23L33/135C12N1/20C12R1/225
CPCA61K35/747A61P39/06A61K8/99A61Q19/08A23L33/135C12N1/20A23V2002/00A23V2400/181A23V2400/175A23V2200/30
Inventor 张红星董晨阳金君华谢远红刘慧
Owner BEIJING UNIV OF AGRI
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