Extraction method for tripterygium glycosides

A technology of triptolide polyglycoside and extraction method, which is applied in the field of natural medicine extraction, can solve the problems of environmental pollution, low purity of triptolide, large amount of solvent, etc., and achieve easy operation, improved extraction rate and purity, and improved stability Effect

Inactive Publication Date: 2020-06-19
HUNAN QIANJIN XIELI PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the microwave extraction process of this method, the amount of extraction solvent is l

Method used

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  • Extraction method for tripterygium glycosides
  • Extraction method for tripterygium glycosides
  • Extraction method for tripterygium glycosides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation method

[0031] (1) The roots of Tripterygium wilfordii are selected and processed into small pieces, and crushed to 60 mesh by a pulverizer to obtain a powder sample.

[0032] (2) Weigh 15 g of the powder sample, add the powder sample to 60% ethanol, the ratio of solid to liquid is 1:20, conduct ultrasonic extraction at 250W, 65°C for 80min, and concentrate the extract to 20% of the volume.

[0033] (3) Add the concentrate obtained in step (2) into chloroform, the ratio of solid to liquid is 1:1.5, microwave extraction at 350W, 61°C for 100min, concentrate the extract to a specific gravity of 1.4, and drain the concentrate to obtain Tripterygium wilfordii Polyglycoside powder.

[0034] 2. Tested

[0035] The yield of tripterygium polyglycosides is 2.87g, and the extraction rate is 19.13%, wherein the purity of triptolide is 98.21%, the purity of triptolide ketone is 97.64%, the purity of triptolide is 97.83%, and the purity of triptolide A The purity ...

Embodiment 2

[0037] (1) The roots of Tripterygium wilfordii are selected and processed into small pieces, and crushed to 80 mesh by a pulverizer to obtain a powder sample.

[0038] (2) Weigh 15 g of the powder sample, add the powder sample to 55% ethanol with a solid-to-liquid ratio of 1:25, conduct ultrasonic extraction at 300W and 75°C for 70 min, and concentrate the extract to 25% of its volume.

[0039] (3) Add the concentrate obtained in step (2) into chloroform, the ratio of solid to liquid is 1:2, microwave extraction at 380W, 62°C for 90 minutes, concentrate the extract to a specific gravity of 1.6, and drain the concentrate to obtain Tripterygium wilfordii Polyglycoside powder.

[0040] 2. Tested

[0041] The yield of tripterygium polyglycosides is 2.72g, and the extraction rate is 18.13%, wherein the purity of triptolide is 98.10%, the purity of triptolide ketone is 97.73%, the purity of triptolide is 97.41%, and the purity of triptolide A The purity is 97.24%.

Embodiment 3

[0043] (1) The root of Tripterygium wilfordii is selected and processed into small pieces, and crushed to 50 mesh by a pulverizer to obtain a powder sample.

[0044] (2) Weigh 15 g of the powder sample, add the powder sample to 50% ethanol, the ratio of solid to liquid is 1:15, ultrasonically extract for 100 min at 200W and 60°C, and concentrate the extract to 15% of the volume.

[0045] (3) Add the concentrate obtained in step (2) into chloroform, the ratio of solid to liquid is 1:1, microwave extraction at 280W, 60°C for 120min, concentrate the extract to a specific gravity of 1.2, and drain the concentrate to obtain Tripterygium wilfordii Polyglycoside powder.

[0046] 2. Tested

[0047] The yield of tripterygium polyglycosides is 2.69g, and the extraction rate is 17.93%, wherein the purity of triptolide is 97.61%, the purity of triptolide ketone is 97.13%, the purity of triptolide is 96.56%, and the purity of triptolide A The purity is 96.71%.

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Abstract

The invention discloses an extraction method for tripterygium glycosides. The method comprises the following steps of: (1) sample pretreatment: cleaning and smashing tripterygium wilfordii Hook. f. roots to obtain a powder sample; (2) crude product extraction: adding the powder sample into 50-60% ethyl alcohol at a solid-liquid ratio of 1:15-25, carrying out ultrasound extraction for 70-100 min under a condition of 200-300 W and 60-75 DEG C, and concentrating an extraction solution to 15-25% of the volume; and (3) crude product purification: adding a concentrate obtained in S (2) into chloroform at a solid-liquid ratio of 1:1-2, carrying out microwave extraction for 90-120 min under a condition of 280-380 W and 60-62 DEG C, and concentrating the extraction solution until specific gravity is 1.2-1.6. According to the method, the use amount of harmful organic solvents can be greatly lowered, an extraction rate of the tripterygium glycosides is obviously improved, the purity of effectiveingredients, including triptolide, triptonide, tripterine and wilforlide A, can be obviously improved, in addition, the extraction method is convenient in an extraction operation and can be easily controlled, and industrial production efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of natural medicine extraction. More specifically, it relates to a method for extracting tripterygium glycosides. Background technique [0002] Tripterygium glycosides (tripterygium glycosides) is a mixture of effective parts extracted and refined from the peeled root of Euonymus plant Tripterygium glycosides, which is the first anti-inflammatory and immune-regulating traditional Chinese medicine extract used in China. Tripterygium wilfordii polyglycosides have a unique curative effect on rheumatoid arthritis, lupus erythematosus, purpura nephritis and other refractory or stubborn diseases, and can also be used for alopecia areata, psoriasis, skin vasculitis, Behcet's disease, etc. Tripterygium wilfordii polyglycosides are widely used, have remarkable curative effects, and have unique therapeutic effects on intractable diseases, so they have gradually become the focus of attention at home and abroad. [00...

Claims

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Application Information

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IPC IPC(8): A61K36/37
CPCA61K36/37A61K2236/13A61K2236/333A61K2236/55
Inventor 邹洋刘如斯廖爱玲谭喜平任超
Owner HUNAN QIANJIN XIELI PHARMA CO LTD
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