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Self-assembled carnosine fluorescent nanoparticles, preparation method and application

A fluorescent nanoparticle, nanoparticle technology, applied in nanotechnology, nanotechnology, nanomedicine, etc., can solve problems such as limiting the application of carnosine, achieve good biocompatibility, improve anti-tumor ability, and improve the effect of stability

Active Publication Date: 2020-06-23
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These factors greatly limit the application of carnosine in the field of tumor therapy

Method used

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  • Self-assembled carnosine fluorescent nanoparticles, preparation method and application
  • Self-assembled carnosine fluorescent nanoparticles, preparation method and application
  • Self-assembled carnosine fluorescent nanoparticles, preparation method and application

Examples

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preparation example Construction

[0030] The invention discloses a method for preparing the above-mentioned self-assembled carnosine fluorescent nanoparticles. The preparation method comprises the following steps:

[0031] Step 1, preparation carnosine monomer and metal ion mixed solution, in described mixed solution, the substance ratio of described carnosine monomer and metal ion is (1-10): 1;

[0032] Step 2, mixing the mixed solution in the step 1 with an organic solvent; adjusting the pH of the total mixed solution to 6-10;

[0033] Step 3, heating the total mixed solution to 60°C-90°C, stirring the reaction, and obtaining the nanoparticle suspension after the reaction is complete;

[0034] Step 4, centrifuging to remove the supernatant to obtain self-assembled carnosine fluorescent nanoparticles.

[0035] Preferably, in above-mentioned step one, the mass ratio of carnosine and metal ion is 2:1; In step two, the volume ratio of mixed solution and organic solvent is 1:(1-9); Total mixed solution pH is 10...

Embodiment 1

[0039] Dissolve 10 mg of carnosine monomer in 1 mL of ultrapure water, and 3.07 g of ZnCl 2 Dissolve in 1mL methanol, the ratio of carnosine to zinc ions is 2:1, mix the two and add 8mL methanol to make the total volume 10mL, adjust the pH to 10 with 1M NaOH solution. Put the reaction solution into a water bath at room temperature, stir and heat to 85°C, evaporate methanol to dryness until the system is 1 mL, and cool to room temperature. After centrifuging at 14000rpm for 10min, discard the supernatant, add 1mL ultrapure water to resuspend, centrifuge again at 14000rpm for 10min, discard the supernatant, add 1mL ultrapure water to resuspend. Observation with an atomic force microscope (AFM), such as figure 1 As shown, it was observed that the diameter of the carnosine fluorescent nanoparticles was about 20nm.

Embodiment 2

[0041] The difference between this embodiment and Example 1 is that copper ions are selected, 10mg carnosine monomer is dissolved in 1mL ultrapure water, 1.42mg CuSO is dissolved in 1mL methanol, the ratio of the amount of carnosine to copper ion 5:1, after the two are mixed, add 4mL of methanol to make the total volume 6mL, adjust the pH to 8 with 1M NaOH solution, put the reaction solution in a water bath at room temperature, stir and heat to 90°C, and evaporate the methanol to dryness. The volume of the system is 1mL, cooled to room temperature, centrifuged, and obtained. Using an atomic force microscope (AFM), it was observed that the diameter of the carnosine fluorescent nanoparticle was about 30nm.

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Abstract

The invention discloses self-assembled carnosine fluorescent nanoparticles. The self-assembled carnosine fluorescent nanoparticles are nanoparticles with regular crystal structures; carnosine monomersare taken as main bodies and coordinate with metal ions through pi-pi accumulation among imidazole rings of the carnosine monomers to form the self-assembled carnosine fluorescent nanoparticles; andthe diameters of the self-assembled carnosine fluorescent nanoparticles are 15 nm to 100 nm. According to the self-assembled carnosine fluorescent nanoparticles, the stability of carnosine is improved, the self-assembled carnosine fluorescent nanoparticles can be slowly degraded after being administrated, and the carnosine is released, so that the acting time of a drug is prolonged.

Description

【Technical field】 [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a self-assembled carnosine fluorescent nanoparticle, a preparation method and an application. 【Background technique】 [0002] Carnosine is a natural dipeptide widely distributed in vertebrate skeletal muscle and brain tissue, composed of one molecule of alanine and one molecule of histidine. The main role of carnosine in skeletal muscle is to maintain cellular homeostasis and regulate muscle excitation-contraction coupling. In addition, carnosine also has many other functions, such as buffering capacity, chelating metal ions, anti-oxidation, and promoting wound healing. It has been confirmed that carnosine can inhibit the proliferation of various cancer cells to a certain extent, which is related to the fact that carnosine can inhibit the aerobic glycolysis of cancer cells. It has also been reported recently that carnosine can make gastric cancer cell mitosis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/05A61K9/51A61K47/52A61K49/00A61P35/00B82Y5/00B82Y20/00
CPCA61K9/5169A61K38/05A61K49/0093A61K47/52A61P35/00B82Y5/00B82Y20/00
Inventor 孙乐明张连兵林炜锋
Owner NORTHWESTERN POLYTECHNICAL UNIV
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