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An artificial dura mater and patch technology, which is used in medical science, tissue regeneration, prosthesis and other directions to achieve the effects of being conducive to the formation of new blood vessels, loose structure, easy degradation and absorption
Active Publication Date: 2020-06-26
江苏白衣缘生物工程有限公司
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Problems solved by technology
[0009] The purpose of the present invention is to carefully analyze some deficiencies and defects in the existing dura mater patches, and combine theory and practice closely based on many years of work experience. Shortcomings of Existing Dural Mesh
Method used
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Examples
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Effect test
Embodiment 1
[0106] Example 1: Preparation of layer D, decellularized SIS (piglets at the end of the nursery period)
[0107] Specific steps are as follows:
[0108]1. Material collection and pretreatment: select piglets at the end of the nursery period (age 60 days, body weight about 22 kg) and slaughter them. The raw material selected is the jejunum section of the fresh small intestine, and the mucous membrane of the small intestine jejunum is removed by mechanical scraping The small intestinal submucosa (SIS) was obtained from the muscular layer, serous layer, lymph nodes, etc.; the isolated SIS was soaked in 0.8% acetic acid solution for 25 minutes, and the ratio of SIS to acetic acid solution in young pigs was 1:6. Soak in purified water to obtain the D-layer film raw material;
[0109] 2. Disinfection: use a mixed aqueous solution containing 1.0% peracetic acid and 15% ethanol, the ratio of the D layer material to the mixed aqueous solution is 1:10, and immerse at room temperature f...
Embodiment 2
[0114] Example 2: Preparation of D layer, decellularized dermis (newborn calf)
[0115] The steps are basically the same as in Example 1, one of the differences: In this example, a newborn calf is taken, the middle skin of the abdomen of the site is taken, fully cleaned, the fat on the inner surface and the outer surface are mechanically removed, rinsed with deionized water, and then washed with deionized water. The skin machine takes dermal tissue with a thickness of 0.15mm;
[0116] The second difference: during raw material pretreatment, use 2.0% acetic acid concentration instead of 0.8% acetic acid, and the rest of the unspecified steps and reagents are exactly the same as in Example 1.
Embodiment 3
[0117] Example 3: Preparation of T layer, decellularized SIS (cultivated sows)
[0118] The steps are basically the same as in Example 1, with the only difference being that sows (gestational age above 8 litters, reared for more than 40 months, body weight about 200 kg, empty pregnant) are selected to be eliminated, and the mucosal layer and mucous layer of the small intestine and jejunum are removed by mechanical scraping. Layer, serosal layer, lymph nodes, etc., to obtain T-layer small intestinal submucosa (SIS) raw materials.
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PUM
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Abstract
The invention relates to an artificial dura compound patch and a preparation method thereof. The artificial dura compound patch at least comprises a T layer and a D layer, wherein the D layer is in direct contact with brain soft tissues, and the T layer is in direct contact with cranial bones; the T layer and the D layer are produced by removing cells and antigens from connective tissues, and carrying out processing; the D layer derived from a pup contains more bioactive factors, has strong capability of inducing regeneration of tissues, has good flexibility and can be well matched with a softbrain tissue surface; the T layer can be derived from ECM of a breeding stock and has an excellent natural degree of crosslinking and a better biomechanical property (good tenacity). The artificial dura compound patch has good tissue regeneration promoting capability and good mechanical properties and is capable of preventing leakage of brain effusion; meanwhile, the artificial dura compound patch can be completely degraded and absorbed, the D layer is firstly degraded, the T layer is slowly degraded, the artificial dura compound patch is accordant with a natural law of regeneration of a dura, and a degraded product has no any toxic and side effects and belongs to an updated dura patch product; and the preparation method of the artificial dura compound patch is simple and practical, and the artificial dura compound patch can be rapidly promoted and popularized and can meets clinical urgent needs.
Description
technical field [0001] The invention belongs to the field of dura mater patches, in particular to a degradable artificial dura mater biocomposite patch and a preparation method thereof. Background technique [0002] Dura (Dura, Dura Mater) is a thick and tough double-layer membrane; the outer layer is the periosteum on the inner surface of the skull, which is only loosely attached to the calvaria, especially in the occipital and temporal parts, and is called the periosteum layer . However, the sutures of the skull and the base of the skull are more firmly attached and difficult to separate. There is no intracranial intradural space. The inner layer of the dura mater is thicker and tougher than the outer layer, and it is continuous with the dura mater at the foramen magnum, called the meningeal layer; its main function is to protect the brain; trauma or craniocerebral surgery can cause dural defects, and surgery is required. Dural patches are used to repair the dura mater ...
Claims
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Application Information
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