Preparation method of nanofiber dermal scaffold

A technology of nanofibers and nanofiber membranes, applied in the field of bioengineering, can solve problems such as reducing the safety of collagen membranes, and achieve the effects of accelerating wound healing, preventing wound infection, and helping to fit

Inactive Publication Date: 2020-06-26
江苏创铭医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the introduction of other polymer materials will have many restrictions on its application, and it will also greatly reduce the safety of collagen membranes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation method of the nanofiber dermal support in this embodiment is as follows:

[0021] (1) dissolving 22wt% human-derived recombinant collagen and 2wt% sodium hyaluronate in hexafluoroisopropanol to prepare a spinning solution;

[0022] (2) Nanofiber membranes are made by electrospinning;

[0023] (3) steam crosslinking the nanofibrous membrane by glutaraldehyde for 6h;

[0024] (4) Load the cross-linked nanofiber membrane on a 200 μm silica gel membrane, and the grammage of the glue is 200g / m 2 , to get nanofiber dermal scaffold, its water vapor transmission rate is 540g / (m 2 24h).

Embodiment 2

[0026] The preparation method of the nanofiber dermal support in this embodiment is as follows:

[0027] (1) dissolving 20wt% human recombinant collagen, 2wt% sodium hyaluronate, and 2wt% chondroitin sulfate in ethanol to prepare a spinning solution;

[0028] (2) Nanofiber membranes are made by electrospinning;

[0029] (3) thermally crosslinking the nanofibrous membrane by glutaraldehyde;

[0030] (4) Load the cross-linked nanofiber membrane on a 50 μm silica gel membrane, and the grammage of the glue is 100g / m 2 , to obtain a nanofibrous dermal scaffold with a water vapor transmission rate of 1000g / (m 2 24h).

Embodiment 3

[0032] The preparation method of the nanofiber dermal support in this embodiment is as follows:

[0033] (1) Dissolving 25wt% human recombinant collagen and 3wt% polyglutamic acid sodium in a mixed solution of purified water and ethanol to prepare a spinning solution;

[0034] (2) Nanofiber membranes are made by electrospinning;

[0035] (3) thermally crosslinking the nanofibrous membrane by glutaraldehyde for 72h;

[0036] (4) Load the cross-linked nanofiber membrane on a 200 μm silica gel membrane, and the grammage of the glue is 300g / m 2 , to get nanofiber dermal scaffold, its water vapor transmission rate is 820g / (m 2 24h).

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method of a nanofiber dermal scaffold. The method includes the following steps: (1) dissolving, by weight, 20-25% of human-derived recombinant collagen and 0-5% of auxiliary materials in a solvent so as to prepare a spinning solution; (2) preparing a nanofiber membrane through electrostatic spinning; (3) cross-linking the nanofiber membrane by using a crosslinking agent; and (4) loading the crosslinked nanofiber membrane on a silica gel membrane so as to obtain the nanofiber dermal scaffold. The prepared scaffold has good fiber morphology and high mechanical strength, needs of medical dressings can be met, and the scaffold can be fitted into damaged tissue parts, and is capable of effectively helping regeneration of dermal tissue, accelerating healing of wounds, maintaining the wound surface moist and reducing risks of scar formation.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a preparation method of a nanofiber dermis support. Background technique [0002] Collagen, also known as collagen, is a helical fibrous protein twisted by three peptide chains. Collagen is an important protein in animal connective tissue. In addition to 60-70% water in connective tissue, collagen accounts for about 20-30%. Because of the high content of collagen, connective tissue has a certain structure and mechanical mechanics Properties, such as tensile strength, tension, elasticity, etc., to achieve the functions of support and protection. [0003] Collagen monomer is a long cylindrical protein with a length of about 280nm and a diameter of 1.4-1.5nm. It consists of three polypeptide chains wound around each other in a supercoiled form. Collagen is composed of a group of proteins with slightly different structures. 27 different types of collagen have be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/60A61L27/24A61L27/20A61L27/18
CPCA61L27/18A61L27/20A61L27/24A61L27/50A61L27/60A61L2400/12C08L77/04C08L5/08
Inventor 莫秀梅冯文浩朱世辉盛嘉隽樊璐露高佩
Owner 江苏创铭医疗器械有限公司
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