Nucleic acid preserving fluid

Abstract

The invention provides nucleic acid preserving fluid. The nucleic acid preserving fluid consists of a nuclease inhibitor, a protein denaturant, a stabilizer, a bacteriostatic agent and a Tricine buffer solution, wherein the concentration of the nuclease inhibitor is 5mg / ml-20mg / ml, the concentration of the protein denaturant is 20mg / ml-80mg / ml, the concentration of the stabilizer is 30mg / ml-90mg / ml, the concentration of the bacteriostatic agent is 100mg / ml-500mg / ml, and the concentration of the Tricine buffer solution is 20nM-120nM. The nucleic acid preserving fluid has the beneficial effectsthat the nucleic acid preserving fluid is capable of effectively preventing degradation of nucleic acid at a normal temperature and providing a stable environment for nucleic acid, so that the preservation time of the nucleic acid is prolonged, and the safety during collection, transportation and detection can be guaranteed; and meanwhile, pathogenic microorganisms can be inactivated, and the nucleic acid preserving fluid has the advantages of low preparation cost, stable performance, easiness in preservation and the like.

Description

technical field [0001] The invention relates to the technical field of nucleic acid, in particular to a nucleic acid preservation solution. Background technique [0002] Respiratory tract infection (RTI) is the most common type of human disease, which can occur in any gender, age and region, and is one of the leading causes of morbidity and death worldwide. The clinical symptoms and signs caused by respiratory tract infection are relatively similar, and its clinical manifestations are mainly rhinitis, pharyngitis, laryngitis, tonsillitis and other symptoms, and severe cases can cause tracheitis, bronchitis and pneumonia, etc. The treatment methods, efficacy and course of the disease also vary. It has been proven that most respiratory diseases are caused by bacteria, viruses and other pathogens, among which respiratory viruses are the most common. Clinically common respiratory pathogens include influenza A virus, influenza B virus, respiratory syncytial virus, parainfluenza...

AI Technical Summary

Problems solved by technology

A variety of respiratory pathogen gene detection kits have been developed based on the above methods, but there are currently no economical, effective, stable and quality control products for a variety of respiratory pathogen gene detection kits on the market, and the existing nucleic acid quality control categories Most of the products have shortcomings such as instability, easy degradation, and difficult storage, and quality control products are important methods and quality indicators to measure and evaluate the performance of a kit such as detection accuracy and stability.

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