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A composition with hypoglycemic effect and its application

A technology for lowering blood sugar and composition, applied in the field of compositions with hypoglycemic effect, can solve the problems of high dosage and limited hypoglycemic effect, and achieve the effects of reducing drug resistance and reducing dosage

Active Publication Date: 2020-12-22
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problem of limited hypoglycemic effect and high dosage of a single natural active ingredient in the prior art, the object of the present invention is to provide a composition with hypoglycemic activity, the active ingredients of which are hesperetin and geranium element; the composition has synergistic inhibition of alpha-glucosidase activity, and has broad application prospects in the preparation of drugs for the treatment of diabetes

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  • A composition with hypoglycemic effect and its application
  • A composition with hypoglycemic effect and its application
  • A composition with hypoglycemic effect and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]A composition with hypoglycemic effect, the active ingredients of which are hesperetin and geranin; the mass concentration ratio of hesperetin and geranin in the composition is 3:10.

[0031]Test of inhibiting α-glucosidase activity in vitro when the mass concentration ratio of hesperetin and geraniol is 3∶10

[0032]1.1 Reagents: α-glucosidase (α-glucosidase, Sigma, 750U), 4-nitrophenyl-α-D-glucopyranoside (pNPG, TOKYO Chemica Industry Co., LTD), Acarbose , TOKYO ChemicaIndustry Co., LTD), corosolic acid, Na2HPO4, NaH2PO4.

[0033]Experimental instrument: Microplate reader TECAN infinite M200 PRO (Teacan Group Ltd., Swizerland).

[0034]1.2 Experimental steps:

[0035]1.2.1 Preparation of drug solution: first prepare hesperetin, geraniol, acarbose and corosolic acid with dimethyl sulfoxide (DMSO) into a mother liquor of 10mg / mL, and use ultrapure water, Na2HPO4And NaH2PO4Prepare 67 mmol / mL phosphate buffer (PBS, pH=6.8), prepare 5 mmol / mL pNPG solution with PBS, and prepare 0.25 U / mL α-gluc...

Embodiment 2

[0067]A composition with hypoglycemic effect, the active ingredients of which are hesperetin and geranin; the mass concentration ratio of hesperetin and geranin in the composition is 6:10.

[0068]Test of inhibiting α-glucosidase activity in vitro when the mass concentration ratio of hesperetin and geraniol is 6∶10

[0069]2.1 Reagents: α-glucosidase (α-glucosidase, Sigma, 750U), 4-nitrophenyl-α-D-glucopyranoside (pNPG, TOKYO Chemica Industry Co., LTD), Acarbose , TOKYO ChemicaIndustry Co., LTD), corosolic acid, Na2HPO4, NaH2PO4.

[0070]Experimental instrument: Microplate reader TECAN infinite M200 PRO (Teacan Group Ltd., Swizerland).

[0071]2.2 Experimental steps:

[0072]2.2.1 Preparation of drug solution: first prepare hesperetin, geraniol, acarbose and corosolic acid with dimethyl sulfoxide (DMSO) into a mother liquor of 10mg / mL, and use ultrapure water, Na2HPO4And NaH2PO4Prepare 67 mmol / mL phosphate buffer (PBS, pH=6.8), prepare 5 mmol / mL pNPG solution with PBS, and prepare 0.25 U / mL α-gluc...

Embodiment 3

[0103]A composition with hypoglycemic effect, the active ingredients of which are hesperetin and geranin; the mass concentration ratio of hesperetin and geranin in the composition is 8:10.

[0104]Test of inhibiting α-glucosidase activity in vitro when the mass concentration ratio of hesperetin and geraniol is 8∶10

[0105]3.1 Reagents: α-glucosidase (α-glucosidase, Sigma, 750U), 4-nitrophenyl-α-D-glucopyranoside (pNPG, TOKYO Chemica Industry Co., LTD), Acarbose , TOKYO ChemicaIndustry Co., LTD), corosolic acid, Na2HPO4, NaH2PO4.

[0106]Experimental instrument: Microplate reader TECAN infinite M200 PRO (Teacan Group Ltd., Swizerland).

[0107]3.2 Experimental steps:

[0108]3.2.1 Preparation of drug solution: first prepare hesperetin, geraniol, acarbose and corosolic acid with dimethyl sulfoxide (DMSO) into a mother liquor of 10mg / mL, and use ultrapure water, Na2HPO4And NaH2PO4Prepare 67 mmol / mL phosphate buffer (PBS, pH=6.8), prepare 5 mmol / mL pNPG solution with PBS, and prepare 0.25 U / mL α-gluc...

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Abstract

The invention discloses a composition with a blood sugar reduction function and application and belongs to the technical field of medicines. The composition with the blood sugar reduction function consists of hesperetin and diosmetin in a mass concentration ratio of (2:10)-(8-10), and preferably the mass concentration ratio of the hesperetin to the diosmetin is (3:10)-(8:10). The composition of the hesperetin and the diosmetin, which is disclosed by the invention, has a remarkable synergism in inhibiting alpha-glucosidase, has an effect prior to those of separately used flavonoids, in addition, is capable of reducing dosages of medicines and reducing drug resistance, and has wide application prospects in preparing medicines, healthcare products or foods for treating diabetes.

Description

Technical field[0001]The invention belongs to the technical field of medicine, and specifically relates to a composition with a blood sugar lowering effect and an application.Background technique[0002]With the development of industrialization, urbanization, population aging and changes in the ecological environment, lifestyle and dietary structure, diabetes has become one of the main chronic diseases that endanger human health worldwide. Its notable features are high blood sugar levels and abnormal lipid, protein and carbohydrate metabolism, which are clinically manifested as "three more and one less" symptoms such as polyphagia, polydipsia, polyuria and weight loss. Studies have shown that α-glucosidase inhibitors can inhibit the activity of α-glucosidase on the brush border of small intestinal villi mucosal cells, and achieve the purpose of controlling postprandial hyperglycemia by blocking the digestion and absorption of carbohydrates. In addition, drugs that have been used in cl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61P3/10A23L33/105
CPCA23V2002/00A61K31/352A23L33/105A61P3/10A61K2300/00A23V2200/328A23V2250/2116
Inventor 张强焦中高刘杰超张春岭刘慧吕真真杨文博陈大磊
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI