Unlock instant, AI-driven research and patent intelligence for your innovation.

Screening method of metabolic marker of drug-induced acute kidney injury lesion process

A technology for metabolic markers and acute kidney injury, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of time-consuming and labor-intensive, and achieve high sensitivity, specificity, non-invasive, and strong specificity

Active Publication Date: 2020-07-14
CHINA PHARM UNIV
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the experiment, the success of drug-induced acute kidney injury model replication can be judged based on 24h urine protein quantification, while the pathological type and degree of renal disease are mainly based on renal histopathological examination, which is not only subjective but also time-consuming and labor-intensive. A dynamic evaluation method for the process of drug-induced acute kidney injury, so it is necessary to establish an overall non-invasive, rapid, high-sensitivity, and specific method that can be used for the diagnosis of acute kidney disease and dynamically reflect the disease course

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Screening method of metabolic marker of drug-induced acute kidney injury lesion process
  • Screening method of metabolic marker of drug-induced acute kidney injury lesion process
  • Screening method of metabolic marker of drug-induced acute kidney injury lesion process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A screening method for metabolic markers of drug-induced acute kidney injury disease process, comprising the following steps:

[0039] The materials needed to prepare for the experiment are as follows:

[0040] Experimental materials and instruments: liquid chromatography; tandem quadrupole-time-of-flight mass spectrometer; Milli-Q ultrapure water system; vortex instrument; centrifuge.

[0041] Experimental reagents: cisplatin; ultrapure water; formic acid; HPLC grade methanol and acetonitrile.

[0042] Experimental animals: SPF grade male ICR mice; the animal breeding room maintains a temperature of (23±1.5)°C and a relative humidity of (45±15)%.

[0043] Step 1: Replication of the drug-induced acute kidney injury model.

[0044] A total of 120 male ICR mice were used to acclimatize to the environment for one week, and the serum of 120 mice was collected within 72 hours, and the serum volume was recorded. The mice were randomly divided into blank control group (grou...

Embodiment 2

[0080] Application of a drug-induced acute kidney injury lesion process metabolic marker obtained by the above screening method in the preparation of drug-induced acute kidney injury lesion process diagnostic and differential reagents.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a screening method of a metabolic marker of a drug-induced acute kidney injury lesion process. A progressive metabolic marker related to the drug-induced acute kidney injury issearched for by adopting a mass spectrum serum metabonomics technology and a mathematical statistics method, the diagnostic capacity of the marker is confirmed by adopting an ROC curve, and finally the metabolic marker reflecting the lesion process of the drug-induced acute kidney injury is determined. The content of endogenous metabolic markers in body serum is changed, metabolic markers reflecting the drug-induced acute kidney injury lesion process are screened, the defects of an existing drug-induced acute kidney injury lesion process evaluation method are overcome, finally, the drug-induced acute kidney injury lesion process is evaluated according to the determined metabolic markers, and the method has the advantages of being quantifiable, rapid, high in sensitivity, high in specificity and noninvasive.

Description

technical field [0001] The invention belongs to the technical field of marker screening and identification, in particular to a screening method for metabolic markers of drug-induced acute kidney injury disease process. Background technique [0002] Drug-induced acute kidney injury is an adverse reaction of the kidneys to therapeutic doses of drugs and a toxic reaction due to drug overdose or irrational application. Drug-induced acute kidney injury can reach 1 / 4 of acute kidney injury, and acute kidney injury can easily lead to chronic kidney injury. Therefore, early recognition and diagnosis of drug-induced acute kidney injury is particularly important to prevent the occurrence of late acute kidney injury. [0003] The cisplatin-induced mouse kidney injury model is an animal model that simulates human kidney disease, and its clinical manifestations are close to the characteristics of drug-induced acute kidney injury. In the experiment, the success of drug-induced acute kid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/88G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/88G01N30/54G01N30/74G01N2030/027G01N2030/062G01N2030/8822
Inventor 田蒋为张王宁吴燕张文泽
Owner CHINA PHARM UNIV