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Salt tolerance and growth promotion effect of strain glutamicibacter soli 1-3-3 and its application

A salt-tolerant and strain-resistant technology, applied in the fields of application, bacteria, and microorganisms, can solve the problems of high cost of physical improvement, secondary soil pollution, and many limiting factors

Active Publication Date: 2021-09-14
SHIHEZI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Physical and chemical improvement methods have many disadvantages, many limiting factors, and high cost of physical improvement. However, if the use of amendments is not standardized in chemical improvement, it is very likely to cause secondary pollution to the soil. Biological improvement is a kind of Environmentally friendly, durable and effective improvement method, which can improve salinized soil through the growth and metabolism of organisms

Method used

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  • Salt tolerance and growth promotion effect of strain glutamicibacter soli 1-3-3 and its application
  • Salt tolerance and growth promotion effect of strain glutamicibacter soli 1-3-3 and its application
  • Salt tolerance and growth promotion effect of strain glutamicibacter soli 1-3-3 and its application

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Embodiment 1

[0033] 1. Medium

[0034] Beef extract-peptone medium: peptone 10g, NaCl 80g, beef extract 3g, agar 20g, pH 7.2-7.4, add distilled water to 1L.

[0035] Ashbeck nitrogen-free medium: mannitol 10g, MgSO 4 ·7H 2 O 0.2g, CaSO 4 2H 2 O0.1g, KH 2 PO 4 0.2g, CaCO 3 5g, 20g agar, pH 7.2-7.4, add distilled water to make up to 1L.

[0036] Phospholytic bacteria screening medium: glucose 10g, (NH 4 ) 2 SO 4 0.5g, KCl 0.3g, MgSO 4 ·7H 2 O0.3g, Ca 3 (PO 4 ) 2 10g, FeSO 4 ·7H 2 O 0.03g, MnSO 4 4H 2 O 0.03g, agar 20g, pH7.0~7.5, add distilled water to make up to 1L.

[0037] Potassium solution medium: Na 2 HPO 4 2g, FeCl 3 0.005g, CaCO 3 0.1g, MgSO 4 ·7H 2 O 0.5g, potassium feldspar powder 1g, sucrose 5g, agar 18g, pH 7.5, add distilled water to make up to 1L.

[0038] Salkowski reaction solution (Fe-HClO 4 ): 1mL0.5mol / L FeCl 3 solution, 50 mL of 35% HCIO 4 solution, mix.

[0039] 2. Detection of life-promoting activity of salt-tolerant strains

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Abstract

The invention provides the effect of salt tolerance and growth promotion of bacterial strain glutamicibacter soli 1‑3‑3 and its application, and belongs to the technical field of microorganisms for salt tolerance and promotion of plant growth. The preservation number of the strain glutamicibacter soli 1-3-3 of the present invention is CCTCC M 20191071, and the NCBI accession number is: MK016481. The bacterial strain glutamicibacter soli 1-3-3 of the present invention has the ability to fix nitrogen, decompose phosphorus and potassium, and produce ACC deaminase; the bacterial strain glutamicibacter soli 1-3-3 of the present invention has growth-promoting effects on different plants .

Description

technical field [0001] The invention belongs to the technical field of microorganisms for salt tolerance and plant growth promotion, and in particular relates to the salt tolerance and growth promotion effect of strain glutamicibacter soli 1-3-3 and its application. Background technique [0002] Xinjiang belongs to the hinterland of the Eurasian continent and has a temperate continental climate. In summer, there is little rainfall and large evaporation, which leads to varying degrees of salinization of the soil in Xinjiang. Soil salinization seriously restricts the growth of economic crops, and is easy to cause diseases and insect pests, reducing the yield and quality of agricultural products, which has become one of the important factors restricting agricultural development. At present, the agricultural improvement methods for salinized soil mainly include physical (deep plowing, irrigation and salt drainage, etc.), chemical (soil improver), biological improvement (halophyt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/01
CPCC05G3/00C12N1/20C12N1/205C12R2001/01
Inventor 孙燕飞王琦琦孙杰刘思洋李杨褚贵新
Owner SHIHEZI UNIVERSITY
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